B2G653 (OTC_LACRJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ornithine carbamoyltransferase
Short name=OTCase
EC=2.1.3.3

Gene names

Name:	arcB
Ordered Locus Names:	LAR_0419

Organism

Lactobacillus reuteri (strain JCM 1112) [Complete proteome] [HAMAP]

Taxonomic identifier

557433 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline. HAMAP MF_01109
Pathway	Amino-acid degradation; L-arginine degradation via ADI pathway; carbamoyl phosphate from L-arginine: step 2/2. HAMAP MF_01109
Subcellular location	Cytoplasm By similarity HAMAP MF_01109.
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Arginine metabolism
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ornithine carbamoyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro ornithine carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Ornithine carbamoyltransferase HAMAP MF_01109		PRO_1000137098
Regions
	Region	57 – 61	5	Carbamoyl phosphate binding By similarity
	Region	273 – 276	4	Ornithine binding By similarity
Sites
	Binding site	108	1	Carbamoyl phosphate By similarity
	Binding site	135	1	Carbamoyl phosphate By similarity
	Site	32	1	Important for structural integrity By similarity
	Site	148	1	Important for structural integrity By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2G653 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: D21F29753A992210	FASTA	335	37,560
	10 20 30 40 50 60 MAFNLRNRSF LTLADFNTRE MEYMLDLAED LKKAKYAGYE GKNLKGKNIA LIFEKSSTRT 70 80 90 100 110 120 RCSFEVGAKD EGAHVTYLGP SGSHIGHKES VKDTARVLGG MFDGIEYRGF SQRNVEILAK 130 140 150 160 170 180 YSGVPVWNGL TDEDHPTQVL ADFLTAHEVL KKPYKDIKFA FVGDGQDNVS NALMLGAAVM 190 200 210 220 230 240 GMEYHVVTPK ELEPTKETLD KANEIAAKTG AKIVVTNDIK EGVKGMDVIY ADVWVSMGES 250 260 270 280 290 300 DDMWEKRINL LKPYQVTMDV MKATENPNVL FEHCLPAFHN LDTEVGKEIE KKFGLKEMEV 310 320 330 TDEVFESEHS VVFREAENRM HTIKAVMVAT LGEQN

References

[1]

"Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production." Morita H., Toh H., Fukuda S., Horikawa H., Oshima K., Suzuki T., Murakami M., Hisamatsu S., Kato Y., Takizawa T., Fukuoka H., Yoshimura T., Itoh K., O'Sullivan D.J., McKay L.L., Ohno H., Kikuchi J., Masaoka T., Hattori M. DNA Res. 15:151-161(2008) [PubMed: 18487258] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JCM 1112.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP007281 Genomic DNA. Translation: BAG24935.1.
RefSeq	YP_001841415.1. NC_010609.1.
3D structure databases
ProteinModelPortal	B2G653.
SMR	B2G653. Positions 2-333.
ModBase	Search...
Protein-protein interaction databases
STRING	B2G653.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6231700.
GenomeReviews	Gene locus LAR_0419 in contig AP007281_GR.
KEGG	lrf:LAR_0419.
PATRIC	22258085. VBILacReu111271_0442.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG579429.
OMA	DFRIFAP.
ProtClustDB	PRK04284.
Family and domain databases
HAMAP	MF_01109. OTCase. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002292. Orn/put_carbamltrans. IPR024904. Orn_carbamltrans. [Graphical view]
KO	K00611.
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00102. OTCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00658. Orni_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTC_LACRJ

Accession

Primary (citable) accession number: B2G653

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	June 10, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program