B2G5M9 (ALR_LACRJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase
EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	LAR_0245

Organism

Lactobacillus reuteri (strain JCM 1112) [Complete proteome] [HAMAP]

Taxonomic identifier

557433 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity. HAMAP MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_01201
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 375	375	Alanine racemase HAMAP MF_01201		PRO_1000138607
Sites
	Active site	40	1	Proton acceptor; specific for D-alanine By similarity
	Active site	268	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	40	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2G5M9 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: C28A06353B077B21	FASTA	375	41,162
	10 20 30 40 50 60 MVNGRLRDTS LIVDLDALRH NIQEQKKVLP ENSKILAVVK ANAYGNGLIP VAQTAMTSGA 70 80 90 100 110 120 SGLCVAILDE ALELRDNGIE AMTLVLGITS VEDALIAAQA GVSLTVGSLD WLEQYHQLAQ 130 140 150 160 170 180 VAKPKKPLKV HLGIDSGMGR IGFTEVAAFK QAVKLLDSPE FEFEGMFTHF ATADSPDENY 190 200 210 220 230 240 FNQQVQRWHQ FVASLAELPP YVHMANSATG LWHRETITAN TIRMGISMYG QNPSGRDLKL 250 260 270 280 290 300 TLDLQPVSSL VSSISFVKQL KAGRSVSYGA TYTAEQDEWL ATLPIGYADG YPRCMTGYKV 310 320 330 340 350 360 LVDGQFCDIA GRVCMDQMMI RLPKYYPVGT PVVLMGKSGD QEITATDLAE RAGTINYEIL 370 TNISNRVHRI YRQSK

References

[1]

"Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production." Morita H., Toh H., Fukuda S., Horikawa H., Oshima K., Suzuki T., Murakami M., Hisamatsu S., Kato Y., Takizawa T., Fukuoka H., Yoshimura T., Itoh K., O'Sullivan D.J., McKay L.L., Ohno H., Kikuchi J., Masaoka T., Hattori M. DNA Res. 15:151-161(2008) [PubMed: 18487258] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JCM 1112.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP007281 Genomic DNA. Translation: BAG24761.1.
RefSeq	YP_001841241.1. NC_010609.1.
3D structure databases
ProteinModelPortal	B2G5M9.
ModBase	Search...
Protein-protein interaction databases
STRING	B2G5M9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6230473.
GenomeReviews	Gene locus LAR_0245 in contig AP007281_GR.
KEGG	lrf:LAR_0245.
PATRIC	22257702. VBILacReu111271_0261.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG712172.
OMA	HYEIACN.
ProtClustDB	CLSK2326072.
Family and domain databases
HAMAP	MF_01201. Ala_racemase. [Tree]
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Gene3D	G3DSA:2.40.37.10. Ala_racemase/Decarboxylase_C. 1 hit.
KO	K01775.
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. Alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_LACRJ

Accession

Primary (citable) accession number: B2G5M9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	June 10, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program