Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bifunctional purine biosynthesis protein PurH

Gene

purH

Organism
Lactobacillus reuteri (strain JCM 1112)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation
IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. IMP cyclohydrolase activity Source: UniProtKB-HAMAP
  2. phosphoribosylaminoimidazolecarboxamide formyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transferase

Keywords - Biological processi

Purine biosynthesis

Enzyme and pathway databases

BioCyciLREU557433:GHNR-140-MONOMER.
UniPathwayiUPA00074; UER00133.
UPA00074; UER00135.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional purine biosynthesis protein PurHUniRule annotation
Including the following 2 domains:
Phosphoribosylaminoimidazolecarboxamide formyltransferaseUniRule annotation (EC:2.1.2.3UniRule annotation)
Alternative name(s):
AICAR transformylaseUniRule annotation
IMP cyclohydrolaseUniRule annotation (EC:3.5.4.10UniRule annotation)
Alternative name(s):
ATICUniRule annotation
IMP synthaseUniRule annotation
InosinicaseUniRule annotation
Gene namesi
Name:purHUniRule annotation
Ordered Locus Names:LAR_0138
OrganismiLactobacillus reuteri (strain JCM 1112)
Taxonomic identifieri557433 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000009150 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 512512Bifunctional purine biosynthesis protein PurHPRO_1000096069Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi557433.LAR_0138.

Structurei

3D structure databases

ProteinModelPortaliB2G5C2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The IMP cyclohydrolase activity resides in the N-terminal region.UniRule annotation

Sequence similaritiesi

Belongs to the PurH family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0138.
HOGENOMiHOG000230373.
KOiK00602.
OMAiPCGVAEG.
OrthoDBiEOG6QCDFF.

Family and domain databases

Gene3Di3.40.140.20. 2 hits.
3.40.50.1380. 1 hit.
HAMAPiMF_00139. PurH.
InterProiIPR024051. AICAR_Tfase_dom.
IPR002695. AICARFT_IMPCHas.
IPR016193. Cytidine_deaminase-like.
IPR011607. MGS-like_dom.
[Graphical view]
PANTHERiPTHR11692. PTHR11692. 1 hit.
PfamiPF01808. AICARFT_IMPCHas. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PIRSFiPIRSF000414. AICARFT_IMPCHas. 1 hit.
SMARTiSM00798. AICARFT_IMPCHas. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMiSSF52335. SSF52335. 1 hit.
SSF53927. SSF53927. 1 hit.
TIGRFAMsiTIGR00355. purH. 1 hit.

Sequencei

Sequence statusi: Complete.

B2G5C2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRALVSVSD KQNLVPFVKG LVENGFEIIS TGGTKRVLDE AGIETIGIED
60 70 80 90 100
VTHFPEILDG RVKTLNPYVH GGLLARRNLP EHMATLEKLN ITPIDLVCVN
110 120 130 140 150
LYPFKETIEK PGVEIADAIE NIDIGGPSMV RSAAKNYHDV TIVVDQADYD
160 170 180 190 200
EVLAQIKEDG ETSLATRARL AAKAFRHTAA YDSLISQYLT KQTGLEDPEK
210 220 230 240 250
LTLSWDLKET MRYGENSHQK AWLYEDALPK AFSVLQAKQL HGKKLSYNNI
260 270 280 290 300
KDADEALRCI REFDEPTVVA MKHMNPCGIG RGDSLVQAWD RAYEADPVSI
310 320 330 340 350
FGGVIALNRQ VDLVTAEKMH KIFLEIVIAP GFDDDAFELL AKKKNIRLLT
360 370 380 390 400
LDFSKKDEPT KHEVVSVMGG MLLQEQDMLK EDYHDWQCVT EKQPTEEQLK
410 420 430 440 450
TLMFAWKAVK HAKSNAIVLA NDERTLGVGE GQPNRIDSLK IAVKHAGEAI
460 470 480 490 500
DDRTVMASDA FFPFGDCVEY AGQNGIKAIV QPGGSVRDQE SIEMANKYGI
510
AMVTTGIRHF RH
Length:512
Mass (Da):57,033
Last modified:June 9, 2008 - v1
Checksum:iECEC7A2970255506
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP007281 Genomic DNA. Translation: BAG24654.1.
RefSeqiYP_001841134.1. NC_010609.1.

Genome annotation databases

EnsemblBacteriaiBAG24654; BAG24654; LAR_0138.
KEGGilrf:LAR_0138.
PATRICi22257458. VBILacReu111271_0147.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP007281 Genomic DNA. Translation: BAG24654.1.
RefSeqiYP_001841134.1. NC_010609.1.

3D structure databases

ProteinModelPortaliB2G5C2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi557433.LAR_0138.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAG24654; BAG24654; LAR_0138.
KEGGilrf:LAR_0138.
PATRICi22257458. VBILacReu111271_0147.

Phylogenomic databases

eggNOGiCOG0138.
HOGENOMiHOG000230373.
KOiK00602.
OMAiPCGVAEG.
OrthoDBiEOG6QCDFF.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00133.
UPA00074; UER00135.
BioCyciLREU557433:GHNR-140-MONOMER.

Family and domain databases

Gene3Di3.40.140.20. 2 hits.
3.40.50.1380. 1 hit.
HAMAPiMF_00139. PurH.
InterProiIPR024051. AICAR_Tfase_dom.
IPR002695. AICARFT_IMPCHas.
IPR016193. Cytidine_deaminase-like.
IPR011607. MGS-like_dom.
[Graphical view]
PANTHERiPTHR11692. PTHR11692. 1 hit.
PfamiPF01808. AICARFT_IMPCHas. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PIRSFiPIRSF000414. AICARFT_IMPCHas. 1 hit.
SMARTiSM00798. AICARFT_IMPCHas. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMiSSF52335. SSF52335. 1 hit.
SSF53927. SSF53927. 1 hit.
TIGRFAMsiTIGR00355. purH. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production."
    Morita H., Toh H., Fukuda S., Horikawa H., Oshima K., Suzuki T., Murakami M., Hisamatsu S., Kato Y., Takizawa T., Fukuoka H., Yoshimura T., Itoh K., O'Sullivan D.J., McKay L.L., Ohno H., Kikuchi J., Masaoka T., Hattori M.
    DNA Res. 15:151-161(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 1112.

Entry informationi

Entry nameiPUR9_LACRJ
AccessioniPrimary (citable) accession number: B2G5C2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 23, 2009
Last sequence update: June 9, 2008
Last modified: March 31, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.