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Protein

Hydroxyacylglutathione hydrolase

Gene

gloB

Organism
Stenotrophomonas maltophilia (strain K279a)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.UniRule annotation

Catalytic activityi

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per subunit.UniRule annotation

Pathwayi: methylglyoxal degradation

This protein is involved in step 2 of the subpathway that synthesizes (R)-lactate from methylglyoxal.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Hydroxyacylglutathione hydrolase (gloB)
This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi52Zinc 1UniRule annotation1
Metal bindingi54Zinc 1UniRule annotation1
Metal bindingi56Zinc 2UniRule annotation1
Metal bindingi57Zinc 2UniRule annotation1
Metal bindingi109Zinc 1UniRule annotation1
Metal bindingi126Zinc 1UniRule annotation1
Metal bindingi126Zinc 2UniRule annotation1
Metal bindingi164Zinc 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00619; UER00676.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyacylglutathione hydrolaseUniRule annotation (EC:3.1.2.6UniRule annotation)
Alternative name(s):
Glyoxalase IIUniRule annotation
Short name:
Glx IIUniRule annotation
Gene namesi
Name:gloBUniRule annotation
Ordered Locus Names:Smlt0995
OrganismiStenotrophomonas maltophilia (strain K279a)
Taxonomic identifieri522373 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeStenotrophomonasStenotrophomonas maltophilia group
Proteomesi
  • UP000008840 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001448161 – 254Hydroxyacylglutathione hydrolaseAdd BLAST254

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi522373.Smlt0995.

Family & Domainsi

Sequence similaritiesi

Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108RW0. Bacteria.
COG0491. LUCA.
HOGENOMiHOG000058041.
KOiK01069.
OMAiDNYIFLL.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01374. Glyoxalase_2. 1 hit.
InterProiIPR032282. HAGH_C.
IPR017782. Hydroxyacylglutathione_Hdrlase.
IPR001279. Metallo-B-lactamas.
[Graphical view]
PfamiPF16123. HAGH_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
[Graphical view]
PIRSFiPIRSF005457. Glx. 1 hit.
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR03413. GSH_gloB. 1 hit.

Sequencei

Sequence statusi: Complete.

B2FR57-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLTALPAFA DNYIWTLIAD DGTAVVVDPG DAAPVLALAD QGLRVDTILL
60 70 80 90 100
THHHDDHIGG VPALQARFPG VRVIAPVEER IPTATGRVGE GERVQALGRT
110 120 130 140 150
FHVLSVPGHT RSHIAFHTAE HLFSGDSLFS LGCGRLFEGT PSQLLASMRK
160 170 180 190 200
LGTLPAQLLL CCAHEYTVSN AVFARHVDPA NAALWQRQEE ALAMRRDDRS
210 220 230 240 250
TLPVTLANEF DCNPFLRVHA APIRASVSAH LGRDVVDDVD VMAGLRHWKD

GFRA
Length:254
Mass (Da):27,591
Last modified:June 10, 2008 - v1
Checksum:i96D75710A9258F15
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM743169 Genomic DNA. Translation: CAQ44559.1.
RefSeqiWP_012479270.1. NC_010943.1.

Genome annotation databases

EnsemblBacteriaiCAQ44559; CAQ44559; Smlt0995.
GeneIDi6392735.
KEGGisml:Smlt0995.
PATRICi23697739. VBISteMal45202_0962.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM743169 Genomic DNA. Translation: CAQ44559.1.
RefSeqiWP_012479270.1. NC_010943.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi522373.Smlt0995.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAQ44559; CAQ44559; Smlt0995.
GeneIDi6392735.
KEGGisml:Smlt0995.
PATRICi23697739. VBISteMal45202_0962.

Phylogenomic databases

eggNOGiENOG4108RW0. Bacteria.
COG0491. LUCA.
HOGENOMiHOG000058041.
KOiK01069.
OMAiDNYIFLL.

Enzyme and pathway databases

UniPathwayiUPA00619; UER00676.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01374. Glyoxalase_2. 1 hit.
InterProiIPR032282. HAGH_C.
IPR017782. Hydroxyacylglutathione_Hdrlase.
IPR001279. Metallo-B-lactamas.
[Graphical view]
PfamiPF16123. HAGH_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
[Graphical view]
PIRSFiPIRSF005457. Glx. 1 hit.
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR03413. GSH_gloB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLO2_STRMK
AccessioniPrimary (citable) accession number: B2FR57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 10, 2008
Last modified: November 2, 2016
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.