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Protein

4-hydroxy-tetrahydrodipicolinate reductase

Gene

dapB

Organism
Stenotrophomonas maltophilia (strain K279a)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate.UniRule annotation

Caution

Was originally thought to be a dihydrodipicolinate reductase (DHDPR), catalyzing the conversion of dihydrodipicolinate to tetrahydrodipicolinate. However, it was shown in E.coli that the substrate of the enzymatic reaction is not dihydrodipicolinate (DHDP) but in fact (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid (HTPA), the product released by the DapA-catalyzed reaction.Curated

Catalytic activityi

(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H.UniRule annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 4 of the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Bifunctional aspartokinase/homoserine dehydrogenase (thrA), Putative lysine-sensitive aspartokinase III (Smlt1168)
  2. Aspartate-semialdehyde dehydrogenase (asd)
  3. 4-hydroxy-tetrahydrodipicolinate synthase (dapA)
  4. 4-hydroxy-tetrahydrodipicolinate reductase (dapB)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei150Proton donor/acceptorUniRule annotation1
Binding sitei151SubstrateUniRule annotation1
Active sitei154Proton donorUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 17NAD(P)UniRule annotation6
Nucleotide bindingi94 – 96NAD(P)UniRule annotation3
Nucleotide bindingi118 – 121NAD(P)UniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Diaminopimelate biosynthesis, Lysine biosynthesis
LigandNAD, NADP

Enzyme and pathway databases

UniPathwayiUPA00034; UER00018

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-tetrahydrodipicolinate reductaseUniRule annotation (EC:1.17.1.8UniRule annotation)
Short name:
HTPA reductaseUniRule annotation
Gene namesi
Name:dapBUniRule annotation
Ordered Locus Names:Smlt2217
OrganismiStenotrophomonas maltophilia (strain K279a)
Taxonomic identifieri522373 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeStenotrophomonasStenotrophomonas maltophilia group
Proteomesi
  • UP000008840 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000940111 – 2394-hydroxy-tetrahydrodipicolinate reductaseAdd BLAST239

Proteomic databases

PRIDEiB2FQ70

Interactioni

Protein-protein interaction databases

STRINGi522373.Smlt2217

Structurei

3D structure databases

SMRiB2FQ70
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni160 – 161Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the DapB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DUK Bacteria
COG0289 LUCA
HOGENOMiHOG000227153
KOiK00215
OMAiEHEVIFA

Family and domain databases

HAMAPiMF_00102 DapB, 1 hit
InterProiView protein in InterPro
IPR022663 DapB_C
IPR000846 DapB_N
IPR022664 DapB_N_CS
IPR023940 DHDPR_bac
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR20836 PTHR20836, 1 hit
PfamiView protein in Pfam
PF05173 DapB_C, 1 hit
PF01113 DapB_N, 1 hit
PIRSFiPIRSF000161 DHPR, 1 hit
SUPFAMiSSF51735 SSF51735, 2 hits
TIGRFAMsiTIGR00036 dapB, 1 hit
PROSITEiView protein in PROSITE
PS01298 DAPB, 1 hit

Sequencei

Sequence statusi: Complete.

B2FQ70-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQTPLRLLI HGASGRMGQA LLRLAAEHPE TLQIAAAVTG RAPAQRVVDG
60 70 80 90 100
VPFFAASELP GAPAFDVAID FSLPEGFDPM LALCVERGAG LVSGTTGISS
110 120 130 140 150
TQRQALEAAA ARIPLVWASN FSLGVAVLDE LVERAAQALA GWDCDIVESH
160 170 180 190 200
HTQKKDAPSG TALTLGAAAQ RGGAEPHYAS LRAGDIVGEH LVQFTGLGER
210 220 230
IELVHRATNR DIFARGALFA ARQLQGRAPG SYRVRDLLQ
Length:239
Mass (Da):25,157
Last modified:June 10, 2008 - v1
Checksum:i4287EC0BB102D83B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM743169 Genomic DNA Translation: CAQ45715.1
RefSeqiWP_005409480.1, NC_010943.1

Genome annotation databases

EnsemblBacteriaiCAQ45715; CAQ45715; Smlt2217
GeneIDi6394132
KEGGisml:Smlt2217

Similar proteinsi

Entry informationi

Entry nameiDAPB_STRMK
AccessioniPrimary (citable) accession number: B2FQ70
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: June 10, 2008
Last modified: May 23, 2018
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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