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B2FPR7 (SYL_STRMK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Smlt3485
OrganismStenotrophomonas maltophilia (strain K279a) [Complete proteome] [HAMAP]
Taxonomic identifier522373 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeStenotrophomonasStenotrophomonas maltophilia group

Protein attributes

Sequence length880 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 880880Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091368

Regions

Motif46 – 5611"HIGH" region HAMAP-Rule MF_00049
Motif638 – 6425"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6411ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B2FPR7 [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: F27102B3E388C6A6

FASTA88098,446
        10         20         30         40         50         60 
MTSAETNAYD PQQVESAAQK YWDATRAFEV DETSDKPKYY CLSMLPYPSG ALHMGHVRNY 

        70         80         90        100        110        120 
TIGDVISRYK RMTGHNVLQP MGWDAFGLPA ENAAIKNKTA PAAWTYKNIE HMRGQFKAMG 

       130        140        150        160        170        180 
YAVDWSREFA TCRPDYYVHE QRMFTRLMRK GLAYRRNAVV NWDPVDQTVL ANEQVIDGRG 

       190        200        210        220        230        240 
WRSGALVEKR EIPQWFLRIT DYAQELLDGL DELDGWPDSV KTMQRNWIGR SEGLEIQFDV 

       250        260        270        280        290        300 
RDVDGTALDP LRVFTTRPDT VMGVTFVSIA AEHPLALHAA KNNPELAALL SEMKQGGVSE 

       310        320        330        340        350        360 
AELETQEKRG MDTGLRAIHP VTGEKVPVWV ANFVLMGYGT GAVMAVPGHD QRDNEVANKY 

       370        380        390        400        410        420 
GLPIKQVIAL KDPRNDDERT WDGARWQDWY SDKNRAFELV NSAEFDGLDF QGAFEALAER 

       430        440        450        460        470        480 
FERKAQGQRR VNYRLRDWGV SRQRYWGCPI PVIYCDKCGA VPVPEEQLPV VLPEDVAFSG 

       490        500        510        520        530        540 
TGSPIKTDPE WRKTTCPECG GAAERETDTF DTFMESSWYY ARYTSPGARD AVDKRGNYWL 

       550        560        570        580        590        600 
PVDQYIGGIE HAILHLMYFR FYHKLLRDAR MVDSNEPARN LLCQGMVIAE TYYRPNPDGS 

       610        620        630        640        650        660 
KDWINPADVE VQRDERGRIT GATLIADGQP VVVGGTEKMS KSKNNGVDPQ AMVDKYGADT 

       670        680        690        700        710        720 
VRLFSMFAAP PEQSLEWNEA GVDGMARFLR RLWAQVQKHA ADGAAPALDV AALDANQKAL 

       730        740        750        760        770        780 
RRKTHETIGK VGDDYGRRHS FNTAIAAVME LMNALAKFED GSEQGRAVRQ EALQAIVLLL 

       790        800        810        820        830        840 
NPITPHASHA LWQVLGHGET LLEDQPFPQA DAGALVRDAL TLAVQVNGKL RGTIEVAADA 

       850        860        870        880 
AREQVEALAL AEPNAAKFME GLTVRKIIIV PGKIVNIVAA 

« Hide

References

[1]"The complete genome, comparative and functional analysis of Stenotrophomonas maltophilia reveals an organism heavily shielded by drug resistance determinants."
Crossman L.C., Gould V.C., Dow J.M., Vernikos G.S., Okazaki A., Sebaihia M., Saunders D., Arrowsmith C., Carver T., Peters N., Adlem E., Kerhornou A., Lord A., Murphy L., Seeger K., Squares R., Rutter S., Quail M.A. expand/collapse author list , Rajandream M.A., Harris D., Churcher C., Bentley S.D., Parkhill J., Thomson N.R., Avison M.B.
Genome Biol. 9:R74.1-R74.13(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K279a.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM743169 Genomic DNA. Translation: CAQ46908.1.
RefSeqYP_001973197.1. NC_010943.1.

3D structure databases

ProteinModelPortalB2FPR7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING522373.Smlt3485.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAQ46908; CAQ46908; Smlt3485.
GeneID6394000.
KEGGsml:Smlt3485.
PATRIC23702433. VBISteMal45202_3269.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycSMAL522373:GJE8-3373-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_STRMK
AccessionPrimary (citable) accession number: B2FPR7
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: June 10, 2008
Last modified: April 16, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries