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B2D0J4 (VDPP4_APIME) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 28. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Venom dipeptidyl peptidase 4
Alternative name(s):
Allergen C
Venom dipeptidyl peptidase IV
EC=3.4.14.5
Allergen=Api m 5
OrganismApis mellifera (Honeybee) [Reference proteome]
Taxonomic identifier7460 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataApoideaApidaeApis

Protein attributes

Sequence length775 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Venom dipeptidyl-peptidase which removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. May process promelittin into its active form and/or modulate the chemotactic activity of immune cells after the insect sting. Ref.1

Catalytic activity

Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline. Ref.1

Enzyme regulation

Inhibited by diprotin A. Ref.1

Subcellular location

Secreted Ref.1.

Tissue specificity

Expressed by the venom duct. Ref.1

Allergenic properties

Causes an allergic reaction in human. Ref.1

Sequence similarities

Belongs to the peptidase S9B family. DPPIV subfamily.

Ontologies

Keywords
   Cellular componentSecreted
   DiseaseAllergen
   DomainSignal
   Molecular functionAminopeptidase
Hydrolase
Protease
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

membrane

Inferred from electronic annotation. Source: InterPro

   Molecular_functionaminopeptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

serine-type peptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 775752Venom dipeptidyl peptidase 4
PRO_5000336987

Sites

Active site6391Charge relay system By similarity
Active site7181Charge relay system By similarity
Active site7501Charge relay system By similarity

Amino acid modifications

Glycosylation681N-linked (GlcNAc...) Potential
Glycosylation2391N-linked (GlcNAc...) Potential
Glycosylation4731N-linked (GlcNAc...) Potential
Glycosylation5051N-linked (GlcNAc...) Potential
Glycosylation5781N-linked (GlcNAc...) Potential
Glycosylation6311N-linked (GlcNAc...) Potential
Glycosylation6891N-linked (GlcNAc...) Potential
Glycosylation6941N-linked (GlcNAc...) Potential
Disulfide bond450 ↔ 453 By similarity
Disulfide bond463 ↔ 481 By similarity
Disulfide bond659 ↔ 770 By similarity

Sequences

Sequence LengthMass (Da)Tools
B2D0J4 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: 7C31C89636EBDEF2

FASTA77587,937
        10         20         30         40         50         60 
MEVLVQLALL LVVHGSLVVL VAGKSVPRVI DQDLERYEPL EEEDHRGARV PFNLEETYDQ 

        70         80         90        100        110        120 
SFRANSFNGT WKTDREILYS DNYVGDIRLF DVTTGSGTVL LDSSVTADFD KASVMFSFDN 

       130        140        150        160        170        180 
SHVAIGHDYV NGFRYSIHQK CTVYNIKSRT FTDIANGDRI PLFKWSPTRN ALIYVHKNDI 

       190        200        210        220        230        240 
YYQVFFEGGS DTRRITNTGV PDIVFNGIPD WVYEEEVLGS PVAFWISPDG RHLAFATFND 

       250        260        270        280        290        300 
TNVRDIVISK YGSPGNSRDQ YPNEIRIKYP KAGTTNPFVS LSVIDLHDPS SKLIDLPPPV 

       310        320        330        340        350        360 
DVVGADNVLY TANWRRDGEI VATWTNRVQN KAQLVLYDTK GNANNIYYEE ETEGWLRIQP 

       370        380        390        400        410        420 
PLYHDRYVIV AKLQDSGTKA GRFLHATRLE YRNGALVDET DLTPGTCEVI SLLLVDHARA 

       430        440        450        460        470        480 
RLYYLGTELG KPSHKNLYSV QLSGNEPPVC LSCDVLTPEG NRCTYAYAYF STNGSHYALY 

       490        500        510        520        530        540 
CAGPDPVFIA IVNANHRQIS IWEENRSLRR KLAARTQPIV KNFNVNANGY TNKVKLYLPP 

       550        560        570        580        590        600 
DFDETKKYPL LITVYAGPNT IRITEEATYG FESYIVTNRS VIYGRIDGRG SAYKGSKMLF 

       610        620        630        640        650        660 
EIYRRLGTVE IEDQIIITRT LQEKYSWIDS NRTGIWGWSY GGFSAAMVLA TDAESVFKCG 

       670        680        690        700        710        720 
ISVAPVTSWI YYDSLYTERF MGLPTPEDNQ SGYNDTDVSR RVEGMRGKKY MLIHGTADDN 

       730        740        750        760        770 
VHYQQTMMLN KALVNSDIMF QQQTYTDEAH ALGNVFPHLY HTTDRFWANC LGYSH 

« Hide

References

« Hide 'large scale' references
[1]"Identification, recombinant expression, and characterization of the 100 kDa high molecular weight hymenoptera venom allergens Api m 5 and Ves v 3."
Blank S., Seismann H., Bockisch B., Braren I., Cifuentes L., McIntyre M., Ruhl D., Ring J., Bredehorst R., Ollert M.W., Grunwald T., Spillner E.
J. Immunol. 184:5403-5413(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 50-63; 76-88; 178-193 AND 606-619, IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ALLERGEN.
Tissue: Venom and Venom duct.
[2]"Insights into social insects from the genome of the honeybee Apis mellifera."
Honeybee genome sequencing consortium
Nature 443:931-949(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EU564832 mRNA. Translation: ACB70230.1.
RefSeqNP_001119715.1. NM_001126243.1.
UniGeneAme.14605.

3D structure databases

ProteinModelPortalB2D0J4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7460.B2D0J4.

Protein family/group databases

Allergome5754. Api m 5.
5755. Api m 5.0101.
MEROPSS09.069.

Proteomic databases

PaxDbB2D0J4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID410337.
KEGGame:410337.

Phylogenomic databases

eggNOGCOG1506.
KOK01278.
PhylomeDBB2D0J4.

Family and domain databases

Gene3D2.140.10.30. 1 hit.
3.40.50.1820. 1 hit.
InterProIPR029058. AB_hydrolase.
IPR001375. Peptidase_S9.
IPR002469. Peptidase_S9B.
[Graphical view]
PfamPF00930. DPPIV_N. 1 hit.
PF00326. Peptidase_S9. 1 hit.
[Graphical view]
SUPFAMSSF53474. SSF53474. 1 hit.
ProtoNetSearch...

Entry information

Entry nameVDPP4_APIME
AccessionPrimary (citable) accession number: B2D0J4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: May 20, 2008
Last modified: June 11, 2014
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Allergens

Nomenclature of allergens and list of entries