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Protein

Venom dipeptidyl peptidase 4

Gene
N/A
Organism
Apis mellifera (Honeybee)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Venom dipeptidyl-peptidase which removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. May process promelittin into its active form and/or modulate the chemotactic activity of immune cells after the insect sting.1 Publication

Catalytic activityi

Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.1 Publication

Enzyme regulationi

Inhibited by diprotin A.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei639 – 6391Charge relay systemBy similarity
Active sitei718 – 7181Charge relay systemBy similarity
Active sitei750 – 7501Charge relay systemBy similarity

GO - Molecular functioni

  1. aminopeptidase activity Source: UniProtKB-KW
  2. serine-type peptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Protein family/group databases

MEROPSiS09.A64.

Names & Taxonomyi

Protein namesi
Recommended name:
Venom dipeptidyl peptidase 4
Alternative name(s):
Allergen C
Venom dipeptidyl peptidase IV (EC:3.4.14.5)
Allergen: Api m 5
OrganismiApis mellifera (Honeybee)
Taxonomic identifieri7460 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataApoideaApidaeApis
ProteomesiUP000005203 Componentsi: Unassembled WGS sequence, Unplaced

Subcellular locationi

  1. Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
  2. membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.1 Publication

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei5754. Api m 5.
5755. Api m 5.0101.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 775752Venom dipeptidyl peptidase 4PRO_5000336987Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi68 – 681N-linked (GlcNAc...)Sequence Analysis
Glycosylationi239 – 2391N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi450 ↔ 453By similarity
Disulfide bondi463 ↔ 481By similarity
Glycosylationi473 – 4731N-linked (GlcNAc...)Sequence Analysis
Glycosylationi505 – 5051N-linked (GlcNAc...)Sequence Analysis
Glycosylationi578 – 5781N-linked (GlcNAc...)Sequence Analysis
Glycosylationi631 – 6311N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi659 ↔ 770By similarity
Glycosylationi689 – 6891N-linked (GlcNAc...)Sequence Analysis
Glycosylationi694 – 6941N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiB2D0J4.

Expressioni

Tissue specificityi

Expressed by the venom duct.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi7460.B2D0J4.

Structurei

3D structure databases

ProteinModelPortaliB2D0J4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S9B family. DPPIV subfamily.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1506.
KOiK01278.
PhylomeDBiB2D0J4.

Family and domain databases

Gene3Di2.140.10.30. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001375. Peptidase_S9.
IPR002469. Peptidase_S9B.
[Graphical view]
PfamiPF00930. DPPIV_N. 1 hit.
PF00326. Peptidase_S9. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B2D0J4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVLVQLALL LVVHGSLVVL VAGKSVPRVI DQDLERYEPL EEEDHRGARV
60 70 80 90 100
PFNLEETYDQ SFRANSFNGT WKTDREILYS DNYVGDIRLF DVTTGSGTVL
110 120 130 140 150
LDSSVTADFD KASVMFSFDN SHVAIGHDYV NGFRYSIHQK CTVYNIKSRT
160 170 180 190 200
FTDIANGDRI PLFKWSPTRN ALIYVHKNDI YYQVFFEGGS DTRRITNTGV
210 220 230 240 250
PDIVFNGIPD WVYEEEVLGS PVAFWISPDG RHLAFATFND TNVRDIVISK
260 270 280 290 300
YGSPGNSRDQ YPNEIRIKYP KAGTTNPFVS LSVIDLHDPS SKLIDLPPPV
310 320 330 340 350
DVVGADNVLY TANWRRDGEI VATWTNRVQN KAQLVLYDTK GNANNIYYEE
360 370 380 390 400
ETEGWLRIQP PLYHDRYVIV AKLQDSGTKA GRFLHATRLE YRNGALVDET
410 420 430 440 450
DLTPGTCEVI SLLLVDHARA RLYYLGTELG KPSHKNLYSV QLSGNEPPVC
460 470 480 490 500
LSCDVLTPEG NRCTYAYAYF STNGSHYALY CAGPDPVFIA IVNANHRQIS
510 520 530 540 550
IWEENRSLRR KLAARTQPIV KNFNVNANGY TNKVKLYLPP DFDETKKYPL
560 570 580 590 600
LITVYAGPNT IRITEEATYG FESYIVTNRS VIYGRIDGRG SAYKGSKMLF
610 620 630 640 650
EIYRRLGTVE IEDQIIITRT LQEKYSWIDS NRTGIWGWSY GGFSAAMVLA
660 670 680 690 700
TDAESVFKCG ISVAPVTSWI YYDSLYTERF MGLPTPEDNQ SGYNDTDVSR
710 720 730 740 750
RVEGMRGKKY MLIHGTADDN VHYQQTMMLN KALVNSDIMF QQQTYTDEAH
760 770
ALGNVFPHLY HTTDRFWANC LGYSH
Length:775
Mass (Da):87,937
Last modified:May 20, 2008 - v1
Checksum:i7C31C89636EBDEF2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU564832 mRNA. Translation: ACB70230.1.
RefSeqiNP_001119715.1. NM_001126243.1.
UniGeneiAme.14605.

Genome annotation databases

GeneIDi410337.
KEGGiame:410337.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU564832 mRNA. Translation: ACB70230.1.
RefSeqiNP_001119715.1. NM_001126243.1.
UniGeneiAme.14605.

3D structure databases

ProteinModelPortaliB2D0J4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7460.B2D0J4.

Protein family/group databases

Allergomei5754. Api m 5.
5755. Api m 5.0101.
MEROPSiS09.A64.

Proteomic databases

PaxDbiB2D0J4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi410337.
KEGGiame:410337.

Phylogenomic databases

eggNOGiCOG1506.
KOiK01278.
PhylomeDBiB2D0J4.

Family and domain databases

Gene3Di2.140.10.30. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001375. Peptidase_S9.
IPR002469. Peptidase_S9B.
[Graphical view]
PfamiPF00930. DPPIV_N. 1 hit.
PF00326. Peptidase_S9. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification, recombinant expression, and characterization of the 100 kDa high molecular weight hymenoptera venom allergens Api m 5 and Ves v 3."
    Blank S., Seismann H., Bockisch B., Braren I., Cifuentes L., McIntyre M., Ruhl D., Ring J., Bredehorst R., Ollert M.W., Grunwald T., Spillner E.
    J. Immunol. 184:5403-5413(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 50-63; 76-88; 178-193 AND 606-619, IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ALLERGEN.
    Tissue: Venom and Venom duct.
  2. "Insights into social insects from the genome of the honeybee Apis mellifera."
    Honeybee genome sequencing consortium
    Nature 443:931-949(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiVDPP4_APIME
AccessioniPrimary (citable) accession number: B2D0J4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: May 20, 2008
Last modified: January 7, 2015
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.