B2D0J4 (VDPP4_APIME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Venom dipeptidyl peptidase 4 Alternative name(s): Allergen C Venom dipeptidyl peptidase IV EC=3.4.14.5 Allergen=Api m 5
Organism	Apis mellifera (Honeybee) [Reference proteome]
Taxonomic identifier	7460 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Hymenoptera › Apocrita › Aculeata › Apoidea › Apidae › Apinae › Apini › Apis

Protein attributes

Sequence length	775 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Venom dipeptidyl-peptidase which removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. May process promelittin into its activ form and/or modulate the chemotactic activity of immune cells after the insect sting. Ref.1
Catalytic activity	Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline. Ref.1
Enzyme regulation	Inhibited by diprotin A. Ref.1
Subcellular location	Secreted Ref.1.
Tissue specificity	Expressed by the venom duct. Ref.1
Allergenic properties	Causes an allergic reaction in human. Ref.1
Sequence similarities	Belongs to the peptidase S9B family. DPPIV subfamily.

Ontologies

Keywords
Cellular component	Secreted
Disease	Allergen
Domain	Signal
Molecular function	Aminopeptidase Hydrolase Protease
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW serine-type peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 775	752	Venom dipeptidyl peptidase 4		PRO_5000336987
Sites
	Active site	639	1	Charge relay system By similarity
	Active site	718	1	Charge relay system By similarity
	Active site	750	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	68	1	N-linked (GlcNAc...) Potential
	Glycosylation	239	1	N-linked (GlcNAc...) Potential
	Glycosylation	473	1	N-linked (GlcNAc...) Potential
	Glycosylation	505	1	N-linked (GlcNAc...) Potential
	Glycosylation	578	1	N-linked (GlcNAc...) Potential
	Glycosylation	631	1	N-linked (GlcNAc...) Potential
	Glycosylation	689	1	N-linked (GlcNAc...) Potential
	Glycosylation	694	1	N-linked (GlcNAc...) Potential
	Disulfide bond	450 ↔ 453		By similarity
	Disulfide bond	463 ↔ 481		By similarity
	Disulfide bond	659 ↔ 770		By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2D0J4 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 7C31C89636EBDEF2	FASTA	775	87,937
	10 20 30 40 50 60 MEVLVQLALL LVVHGSLVVL VAGKSVPRVI DQDLERYEPL EEEDHRGARV PFNLEETYDQ 70 80 90 100 110 120 SFRANSFNGT WKTDREILYS DNYVGDIRLF DVTTGSGTVL LDSSVTADFD KASVMFSFDN 130 140 150 160 170 180 SHVAIGHDYV NGFRYSIHQK CTVYNIKSRT FTDIANGDRI PLFKWSPTRN ALIYVHKNDI 190 200 210 220 230 240 YYQVFFEGGS DTRRITNTGV PDIVFNGIPD WVYEEEVLGS PVAFWISPDG RHLAFATFND 250 260 270 280 290 300 TNVRDIVISK YGSPGNSRDQ YPNEIRIKYP KAGTTNPFVS LSVIDLHDPS SKLIDLPPPV 310 320 330 340 350 360 DVVGADNVLY TANWRRDGEI VATWTNRVQN KAQLVLYDTK GNANNIYYEE ETEGWLRIQP 370 380 390 400 410 420 PLYHDRYVIV AKLQDSGTKA GRFLHATRLE YRNGALVDET DLTPGTCEVI SLLLVDHARA 430 440 450 460 470 480 RLYYLGTELG KPSHKNLYSV QLSGNEPPVC LSCDVLTPEG NRCTYAYAYF STNGSHYALY 490 500 510 520 530 540 CAGPDPVFIA IVNANHRQIS IWEENRSLRR KLAARTQPIV KNFNVNANGY TNKVKLYLPP 550 560 570 580 590 600 DFDETKKYPL LITVYAGPNT IRITEEATYG FESYIVTNRS VIYGRIDGRG SAYKGSKMLF 610 620 630 640 650 660 EIYRRLGTVE IEDQIIITRT LQEKYSWIDS NRTGIWGWSY GGFSAAMVLA TDAESVFKCG 670 680 690 700 710 720 ISVAPVTSWI YYDSLYTERF MGLPTPEDNQ SGYNDTDVSR RVEGMRGKKY MLIHGTADDN 730 740 750 760 770 VHYQQTMMLN KALVNSDIMF QQQTYTDEAH ALGNVFPHLY HTTDRFWANC LGYSH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification, recombinant expression, and characterization of the 100 kDa high molecular weight hymenoptera venom allergens Api m 5 and Ves v 3." Blank S., Seismann H., Bockisch B., Braren I., Cifuentes L., McIntyre M., Ruhl D., Ring J., Bredehorst R., Ollert M.W., Grunwald T., Spillner E. J. Immunol. 184:5403-5413(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 50-63; 76-88; 178-193 AND 606-619, MASS SPECTROMETRY, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ALLERGEN. Tissue: Venom and Venom duct.
[2]	"Insights into social insects from the genome of the honeybee Apis mellifera." Honeybee genome sequencing consortium Nature 443:931-949(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	EU564832 mRNA. Translation: ACB70230.1.
RefSeq	NP_001119715.1. NM_001126243.1.
UniGene	Ame.14605.
3D structure databases
ProteinModelPortal	B2D0J4.
ModBase	Search...
Protein family/group databases
Allergome	5754. Api m 5. 5755. Api m 5.0101.
MEROPS	S09.069.
Proteomic databases
PaxDb	B2D0J4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	410337.
KEGG	ame:410337.
Phylogenomic databases
eggNOG	COG1506.
KO	K01278.
PhylomeDB	B2D0J4.
Family and domain databases
InterPro	IPR001375. Peptidase_S9. IPR002469. Peptidase_S9B. [Graphical view]
Pfam	PF00930. DPPIV_N. 1 hit. PF00326. Peptidase_S9. 1 hit. [Graphical view]
PROSITE	PS00708. PRO_ENDOPEP_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VDPP4_APIME

Accession

Primary (citable) accession number: B2D0J4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 30, 2010
Last sequence update:	May 20, 2008
Last modified:	November 28, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)