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B2D0J4

- VDPP4_APIME

UniProt

B2D0J4 - VDPP4_APIME

Protein

Venom dipeptidyl peptidase 4

Gene
N/A
Organism
Apis mellifera (Honeybee)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 29 (01 Oct 2014)
      Sequence version 1 (20 May 2008)
      Previous versions | rss
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    Functioni

    Venom dipeptidyl-peptidase which removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. May process promelittin into its active form and/or modulate the chemotactic activity of immune cells after the insect sting.1 Publication

    Catalytic activityi

    Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.1 Publication

    Enzyme regulationi

    Inhibited by diprotin A.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei639 – 6391Charge relay systemBy similarity
    Active sitei718 – 7181Charge relay systemBy similarity
    Active sitei750 – 7501Charge relay systemBy similarity

    GO - Molecular functioni

    1. aminopeptidase activity Source: UniProtKB-KW
    2. serine-type peptidase activity Source: InterPro

    Keywords - Molecular functioni

    Aminopeptidase, Hydrolase, Protease

    Protein family/group databases

    MEROPSiS09.069.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Venom dipeptidyl peptidase 4
    Alternative name(s):
    Allergen C
    Venom dipeptidyl peptidase IV (EC:3.4.14.5)
    Allergen: Api m 5
    OrganismiApis mellifera (Honeybee)
    Taxonomic identifieri7460 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataApoideaApidaeApis
    ProteomesiUP000005203: Unplaced

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    2. membrane Source: InterPro

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Allergenic propertiesi

    Causes an allergic reaction in human.1 Publication

    Keywords - Diseasei

    Allergen

    Protein family/group databases

    Allergomei5754. Api m 5.
    5755. Api m 5.0101.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 775752Venom dipeptidyl peptidase 4PRO_5000336987Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi68 – 681N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi239 – 2391N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi450 ↔ 453By similarity
    Disulfide bondi463 ↔ 481By similarity
    Glycosylationi473 – 4731N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi505 – 5051N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi578 – 5781N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi631 – 6311N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi659 ↔ 770By similarity
    Glycosylationi689 – 6891N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi694 – 6941N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiB2D0J4.

    Expressioni

    Tissue specificityi

    Expressed by the venom duct.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi7460.B2D0J4.

    Structurei

    3D structure databases

    ProteinModelPortaliB2D0J4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase S9B family. DPPIV subfamily.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1506.
    KOiK01278.
    PhylomeDBiB2D0J4.

    Family and domain databases

    Gene3Di2.140.10.30. 1 hit.
    3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR001375. Peptidase_S9.
    IPR002469. Peptidase_S9B.
    [Graphical view]
    PfamiPF00930. DPPIV_N. 1 hit.
    PF00326. Peptidase_S9. 1 hit.
    [Graphical view]
    SUPFAMiSSF53474. SSF53474. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B2D0J4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEVLVQLALL LVVHGSLVVL VAGKSVPRVI DQDLERYEPL EEEDHRGARV    50
    PFNLEETYDQ SFRANSFNGT WKTDREILYS DNYVGDIRLF DVTTGSGTVL 100
    LDSSVTADFD KASVMFSFDN SHVAIGHDYV NGFRYSIHQK CTVYNIKSRT 150
    FTDIANGDRI PLFKWSPTRN ALIYVHKNDI YYQVFFEGGS DTRRITNTGV 200
    PDIVFNGIPD WVYEEEVLGS PVAFWISPDG RHLAFATFND TNVRDIVISK 250
    YGSPGNSRDQ YPNEIRIKYP KAGTTNPFVS LSVIDLHDPS SKLIDLPPPV 300
    DVVGADNVLY TANWRRDGEI VATWTNRVQN KAQLVLYDTK GNANNIYYEE 350
    ETEGWLRIQP PLYHDRYVIV AKLQDSGTKA GRFLHATRLE YRNGALVDET 400
    DLTPGTCEVI SLLLVDHARA RLYYLGTELG KPSHKNLYSV QLSGNEPPVC 450
    LSCDVLTPEG NRCTYAYAYF STNGSHYALY CAGPDPVFIA IVNANHRQIS 500
    IWEENRSLRR KLAARTQPIV KNFNVNANGY TNKVKLYLPP DFDETKKYPL 550
    LITVYAGPNT IRITEEATYG FESYIVTNRS VIYGRIDGRG SAYKGSKMLF 600
    EIYRRLGTVE IEDQIIITRT LQEKYSWIDS NRTGIWGWSY GGFSAAMVLA 650
    TDAESVFKCG ISVAPVTSWI YYDSLYTERF MGLPTPEDNQ SGYNDTDVSR 700
    RVEGMRGKKY MLIHGTADDN VHYQQTMMLN KALVNSDIMF QQQTYTDEAH 750
    ALGNVFPHLY HTTDRFWANC LGYSH 775
    Length:775
    Mass (Da):87,937
    Last modified:May 20, 2008 - v1
    Checksum:i7C31C89636EBDEF2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EU564832 mRNA. Translation: ACB70230.1.
    RefSeqiNP_001119715.1. NM_001126243.1.
    UniGeneiAme.14605.

    Genome annotation databases

    GeneIDi410337.
    KEGGiame:410337.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EU564832 mRNA. Translation: ACB70230.1 .
    RefSeqi NP_001119715.1. NM_001126243.1.
    UniGenei Ame.14605.

    3D structure databases

    ProteinModelPortali B2D0J4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7460.B2D0J4.

    Protein family/group databases

    Allergomei 5754. Api m 5.
    5755. Api m 5.0101.
    MEROPSi S09.069.

    Proteomic databases

    PaxDbi B2D0J4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 410337.
    KEGGi ame:410337.

    Phylogenomic databases

    eggNOGi COG1506.
    KOi K01278.
    PhylomeDBi B2D0J4.

    Family and domain databases

    Gene3Di 2.140.10.30. 1 hit.
    3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR001375. Peptidase_S9.
    IPR002469. Peptidase_S9B.
    [Graphical view ]
    Pfami PF00930. DPPIV_N. 1 hit.
    PF00326. Peptidase_S9. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53474. SSF53474. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Identification, recombinant expression, and characterization of the 100 kDa high molecular weight hymenoptera venom allergens Api m 5 and Ves v 3."
      Blank S., Seismann H., Bockisch B., Braren I., Cifuentes L., McIntyre M., Ruhl D., Ring J., Bredehorst R., Ollert M.W., Grunwald T., Spillner E.
      J. Immunol. 184:5403-5413(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 50-63; 76-88; 178-193 AND 606-619, IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ALLERGEN.
      Tissue: Venom and Venom duct.
    2. "Insights into social insects from the genome of the honeybee Apis mellifera."
      Honeybee genome sequencing consortium
      Nature 443:931-949(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiVDPP4_APIME
    AccessioniPrimary (citable) accession number: B2D0J4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 30, 2010
    Last sequence update: May 20, 2008
    Last modified: October 1, 2014
    This is version 29 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Allergens
      Nomenclature of allergens and list of entries
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3