Reviewed, UniProtKB/Swiss-Prot B2C4J3 (L_CHAVB)

Names and origin

Protein names

Recommended name:
    RNA-directed RNA polymerase
    EC=2.7.7.48
Alternative name(s):
    Protein L

Gene names

Name:

L

Organism

Chapare virus (isolate Human/Bolivia/810419/2003)

Taxonomic identifier

499556 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Arenaviridae › Arenavirus › New world arenaviruses › unclassified New world arenaviruses

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	2208 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome. During transcription, synthesizes 4 subgenomic RNAs, and assures their capping by a cap-snatching mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Homomultimerizes; the oligomeric structure is essential for the polymerase activity. Interacts with the nucleocapsid protein N By similarity. Interacts with protein Z; this interaction inhibits viral transcription and replication By similarity.
Subcellular location	Virion. Host cytoplasm.
Sequence similarities	Belongs to the arenaviridae RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Cellular component	Host cytoplasm Virion
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Gene Ontology (GO)
Biological process	transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2208	2208	RNA-directed RNA polymerase		PRO_0000361634
Regions
	Domain	1171 – 1367	197	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	B2C4J3-1 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 01FBA91DEC3CA6B0	FASTA	2,208	252,825
	10 20 30 40 50 60 MDTFLLELKD LVRKYVPELV ELSFQKDALL SQVHPRLVLV EGFKLLSLLV ELESCKVNAC 70 80 90 100 110 120 RHNFEQKFVD VILSDHGVIC PTLPKVTPDG FNLMGKTLIL LETFVRVNPN DFERKWKADM 130 140 150 160 170 180 SKLMSLKDDL ARVGITMVPV VDGRGSYNTS YLPEWATERL RWLLIEILKG VKATSEIEIE 190 200 210 220 230 240 DQEYQRLIHS LAKANNQSMG FENLEFLKRR LLSYDQLLDT SLLVGIRNDV RESKIIEELI 250 260 270 280 290 300 KIKLWYKTEI FNKGLGKFKR TNKSNLLSDL LKIGLHQDSD TINCMFCSCK ILELCYTLSN 310 320 330 340 350 360 KLSIDHSKEE MKDDEVGGKQ PVCISYSSLL SICNKIKGSK IFNTRRNTLL FLDLIMLNFI 370 380 390 400 410 420 VDEMIQDDSV VDSLRGAGFI IGQMVVLVND RALDILAAMK LIRHKLGNSK DWLSVCGKVL 430 440 450 460 470 480 KRYDEEMWKE VKTYIKEPDF DMLFQLAKSL VSERPIMRYT VHKDNESRCL HQNSLNISDQ 490 500 510 520 530 540 SFKAMLKALS HVSLSLINSM KTSFSSRLLI NEKDYSRYYG NVRLKECYVQ RFPISSRVTG 550 560 570 580 590 600 YLFYQKTGER SRCYSLYISE NGELSELGSF YCDPKRFFLP VFSEDTIVSM CNEMVCWLDF 610 620 630 640 650 660 DEQLVELVKP KLRSLVLLLL CSPSKRNQTF IQGLRYFIMA YANQAHHVDL MSKLEIECKS 670 680 690 700 710 720 SSEIQLQRLA VTLFELVLST GDDKDFGFAR RFKFLLNISY LCHFVTKETP DRLTDQIKCF 730 740 750 760 770 780 EKFLEPKLNF NSVIVNPSLS GILTEAQEEI MTSSVNRFFQ KNLTNISDVK EPGVSKELIS 790 800 810 820 830 840 FCVSLFNRGK LRVSGDLKVD PFRPSFTSTA LDLSSNKSVV VPKLDELGNA LSKYDKQMMI 850 860 870 880 890 900 SSCVTTLTEM FKTKGRYNLD PDSLDFLVLK NLSNLVSVSV SKGQMKEELS LLYDTLSEEQ 910 920 930 940 950 960 LESFEQIKQD VQLTLSRMKE SKCNNVGLGN SRKVNKHLSK SELLETLWSP YQVLRAIKNE 970 980 990 1000 1010 1020 VSIHEIKDFD PDIIEHETVK KLCDEVYQSS NKLEFFLEEP LKSVPLEFLL KNLTTIAYEE 1030 1040 1050 1060 1070 1080 TDYLECFKYL LIQGGFDQKL GSYEHKSRSR LGLSSEALRV QEDARVSTRE SNAEAIAKKL 1090 1100 1110 1120 1130 1140 DKTFFTSAAL RNLCFYSEDS PTEFTSVSTN TGNLKFGLSY KEQVGSNREL YVGDLNTKLM 1150 1160 1170 1180 1190 1200 TRLVEDFSEM ITSSMRYSCL NSEKEFERAI CDMKMAVNNG DISMSLDHSK WGPHMSPALF 1210 1220 1230 1240 1250 1260 YSFLANLNLT EPKSRAKLNL GPLLDILKWH LHKVVEVPFN VAQAYCVGKI KRSLGLMECQ 1270 1280 1290 1300 1310 1320 TSSVTEQFYH NFLQRENEIP SHIMSVLDMG QGILHNLSDL YALITEQFLN YAIYKLYDVD 1330 1340 1350 1360 1370 1380 VLSYTSSDDQ ISIMKLPAYE HIDEDSPDWL EIVCFHEYLS SKLNKFVSPK SVVGNFVAEF 1390 1400 1410 1420 1430 1440 KSRFFVMGEE TPLLTKFVAA ALHNVRCKTP TQLAETVDTI CDQCVANGVS VSIVSKISER 1450 1460 1470 1480 1490 1500 VNRLVKYSGF GETPFLSVVK QDVKDWSDGS RGYRLQRNIE NSLRDSKILE VMRKGARKVF 1510 1520 1530 1540 1550 1560 LGIKNGRIFE ENLIGLIGRG GDEALRGFLL YAEVDKDEIE NALRYRWVNT STFGDLRLVL 1570 1580 1590 1600 1610 1620 RTKIMSSKRV LERESIPSLV KTLQSRMSKN FTKGAKKILA ESINKSAFQS SVASGFIGFC 1630 1640 1650 1660 1670 1680 KSMGSKCVRD GSGGFIYLKD IYKKITTCEC KHCSVWRGVV YCEKSVEKIF QFTRSIMWDY 1690 1700 1710 1720 1730 1740 FTLVLTNACE LGEWVFSSVK LPTKATILDN PNLFWAIKPR THKHIEDRLG LNHILHSIKK 1750 1760 1770 1780 1790 1800 NYPQLFEEHL APFMSDLQSN QMINPSKIKF LDICVALDMV NENLGIIGHL LRGRNNTIYI 1810 1820 1830 1840 1850 1860 VKQSECAGAH VRQADYVDQD LGLSPQQICY NFKVQFLLSS MINPLIVSTS TLRSFFWFNE 1870 1880 1890 1900 1910 1920 VLSIEEDDQI ELGELTDFTL SIKTYNLERA MTLDDMTMGY VCSTLLDEVV SLESLDSCQD 1930 1940 1950 1960 1970 1980 LAALQFKRQD LSDFFRDLGE DFVKVGLNIQ IVHQRRSTKF DISRKVVYTF RILLLINLSE 1990 2000 2010 2020 2030 2040 HLREEVKIPV QSLSLYASGA GNNHLFLDGV SMIPTLPLFN GSKSVNLAKV LIEHELATSN 2050 2060 2070 2080 2090 2100 DFKLLECVIM DFSNFLDELR DKYSYVLVGP EEQENPIVFQ NGAFMADNQK LSYMRVEIFG 2110 2120 2130 2140 2150 2160 DTIVKALGAL ETDREIENLL CNLWPYLKSI KKTIDFNQAD FEMIYDLHRT ALLKSLCQMD 2170 2180 2190 2200 SWIEFTSFSV AYSKHLQDLV VSDNLGNLRL KGITCRPFRR DQCIQEIE

References

[1]

"Chapare virus, a newly discovered arenavirus isolated from a fatal hemorrhagic fever case in Bolivia." Delgado S., Erickson B.R., Agudo R., Blair P.J., Vallejo E., Albarino C.G., Vargas J., Comer J.A., Rollin P.E., Ksiazek T.G., Olson J.G., Nichol S.T. PLoS Pathog. 4:E1000047-E1000047(2008) [PubMed: 18421377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	EU260464 Genomic RNA. Translation: ABY87071.1.
RefSeq	YP_001816785.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6216305.
Family and domain databases
InterPro	IPR014386. RNA-dir_pol_arenavirus. IPR007099. RNA-dir_pol_NSvirus. IPR010453. RNA_pol_arenavir. [Graphical view]
Pfam	PF06317. Arena_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF000836. L_ArenaV. 1 hit.
PROSITE	PS50525. RDRP_SSRNA_NEG_SEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_CHAVB

Accession

Primary (citable) accession number: B2C4J3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	May 20, 2008
Last modified:	November 3, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)