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B2BMF8 (VP2_ROTW3) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 16. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein attributes

Sequence length880 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Inner capsid protein that self assembles to form an icosahedral capsid with a T=2 symmetry, which consists of 120 copies of VP2, with channels at each of its five-fold vertices. This capsid constitutes the innermost concentric layer of the viral mature particle. It encapsidates the polymerase VP1, the capping enzyme VP3 and the genomic dsRNA, thereby defining the core. The innermost VP2 capsid and the intermediate VP6 capsid remain intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nacent transcripts are transcribed within the structural confines of this double-layered particle (DLP) and are extruded through the channels at the five-fold axes. VP2 is required for the replicase activity of VP1 polymerase. It probably plays a role in the coordination of packaging and genome replication by controlling the initiation of minus-strand synthesis. Binding to the polymerase VP1 presumably activates the autoinhibited VP1-RNA complex which will start the synthesis of the complementary minus-strand By similarity.

Subunit structure

Dimer Potential. Interacts with VP1; this interaction presumably activates VP1. Interacts with VP3. Interacts with VP6. Interacts with NSP5 By similarity.

Subcellular location

Virion Potential. Note: Inner capsid protein. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.

Domain

The N-terminus is involved in VP1 binding By similarity.

Sequence similarities

Belongs to the rotavirus VP2 family.

Ontologies

Keywords
   Cellular componentCapsid protein
Inner capsid protein
Virion
   DomainRepeat
   LigandRNA-binding
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentviral inner capsid

Inferred from electronic annotation. Source: UniProtKB-KW

viral nucleocapsid

Inferred from electronic annotation. Source: InterPro

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 880880Inner capsid protein VP2
PRO_0000368054

Regions

Region1 – 9292Interaction with VP1 and VP3 By similarity
Region394 – 41421Hydrophobic By similarity
Region422 – 44221Hydrophobic By similarity
Region536 – 55722Leucine-zipper 1 Potential
Region665 – 68622Leucine-zipper 2 Potential

Sequences

Sequence LengthMass (Da)Tools
B2BMF8 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: 02CBB43F26B4FE67

FASTA880102,511
        10         20         30         40         50         60 
MAYRKRGARR EANINNNDRM QEKDDEKQDQ NNRMQLSDKV LSKKEEVVTD NQEEIKIADE 

        70         80         90        100        110        120 
VKKSTKEESK QLLEVLKTKE EHQKEIQYEI LQKTIPTFEP KESILKKLED IKPEQAKKQT 

       130        140        150        160        170        180 
KLFRIFEPRQ LPIYRANGEK ELRNRWYWKL KKDTLPDGDY DVREYFLNLY DQVLTEMPDY 

       190        200        210        220        230        240 
LLLKDMAVEN KNSRDAGKVV DSETASICDA IFQDEETEGA VRRFIAEMRQ RVQADRNVVN 

       250        260        270        280        290        300 
YPSILHPIDY AFNEYFLQHQ LVEPLNNDII FNYIPERIRN DVNYILNMDR NLPSTARYIR 

       310        320        330        340        350        360 
PNLLQDRLNL HDNFESLWDT ITTSNYILAR SVVPDLKELV STEAQIQKMS QDLQLEALTI 

       370        380        390        400        410        420 
QSETQFLTGI NSQAANDCFK TLIAAMLSQR TMSLDFVTTN YMSLISGMWL LTVVPNDMFI 

       430        440        450        460        470        480 
RESLVACQLA IVNTIIYPAF GMQRMHYRNG DPQTPFQIAE QQIQNFQVAN WLHFVNNNQF 

       490        500        510        520        530        540 
RQVVIDGVLN QVLNDNIRDG HVINQLMEAL MQLSRQQFPT MPVDYKRSIQ RGILLLSNRL 

       550        560        570        580        590        600 
GQLVDLTRLL AYNYETLMAC VTMNMQHVQT LTTEKLQLTS VTSLCMLIGN ATVIPSPQTL 

       610        620        630        640        650        660 
FHYYNVNVNF HSNYNERIND AVAIITAANR LNLYQKKMKA IVEDFLKRLH IFDVARVPDD 

       670        680        690        700        710        720 
QMYRLRDRLR LLPVEVRRLD IFNLILMNMD QIERASDKIA QGVIIAYRDM QLERDEMYGY 

       730        740        750        760        770        780 
VNIARNLDGF QQINLEELMR TGDYAQITNM LLNNQPVALV GALPFVTDSS VISLIAKLDA 

       790        800        810        820        830        840 
TVFAQIVKLR KVDTLKPILY KINSDSNDFY LVANYDWVPT STTKVYKQVP QQFDFRNSMH 

       850        860        870        880 
MLTSNLTFTV YSDLLAFVSA DTVEPINAVA FDNMRIMNEL 

« Hide

References

[1]"Full genome-based classification of rotaviruses reveals a common origin between human Wa-Like and porcine rotavirus strains and human DS-1-like and bovine rotavirus strains."
Matthijnssens J., Ciarlet M., Heiman E.M., Arijs I., Delbeke T., McDonald S.M., Palombo E.A., Iturriza-Gomara M., Maes P., Patton J.T., Rahman M., Van Ranst M.
J. Virol. 82:3204-3219(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EF560616 Genomic RNA. Translation: ABU48677.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR007779. Rotavirus_VP2.
[Graphical view]
PfamPF05087. Rota_VP2. 1 hit.
[Graphical view]
ProDomPD008866. Rota_VP2. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Entry information

Entry nameVP2_ROTW3
AccessionPrimary (citable) accession number: B2BMF8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: May 20, 2008
Last modified: October 16, 2013
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families