B2ATB0 (LCPS_PODAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Bifunctional lycopene cyclase/phytoene synthase Including the following 2 domains: Lycopene beta-cyclase EC=5.5.1.19 Alternative name(s): Lycopene cyclase Phytoene synthase EC=2.5.1.32
Organism	Podospora anserina (strain S / ATCC MYA-4624 / DSM 980 / FGSC 10383) (Pleurage anserina) [Complete proteome]
Taxonomic identifier	515849 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Sordariomycetidae › Sordariales › Lasiosphaeriaceae › Podospora › Podospora anserina

Protein attributes

Sequence length	593 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes the reactions from geranylgeranyl diphosphate to phytoene (phytoene synthase) and lycopene to beta-carotene via the intermediate gamma-carotene (lycopene cyclase) By similarity.
Catalytic activity	Carotenoid psi-end group = carotenoid beta-end group. 2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.
Pathway	Carotenoid biosynthesis; beta-carotene biosynthesis. Carotenoid biosynthesis; phytoene biosynthesis; all-trans-phytoene from geranylgeranyl diphosphate: step 1/1.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	In the N-terminal section; belongs to the lycopene beta-cyclase family. In the C-terminal section; belongs to the phytoene/squalene synthase family.

Ontologies

Keywords
Biological process	Carotenoid biosynthesis
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Isomerase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological_process	carotenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	geranylgeranyl-diphosphate geranylgeranyltransferase activity Inferred from electronic annotation. Source: EC intramolecular lyase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 593	593	Bifunctional lycopene cyclase/phytoene synthase		PRO_0000409241
Regions
	Transmembrane	8 – 28	21	Helical; Potential
	Transmembrane	31 – 51	21	Helical; Potential
	Transmembrane	77 – 97	21	Helical; Potential
	Transmembrane	117 – 136	20	Helical; Potential
	Transmembrane	147 – 167	21	Helical; Potential
	Transmembrane	169 – 189	21	Helical; Potential
	Transmembrane	231 – 251	21	Helical; Potential
	Region	1 – 242	242	Lycopene beta-cyclase
	Region	249 – 593	345	Phytoene synthase

Sequences

	Sequence		Length	Mass (Da)
	B2ATB0 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: B7430708736FDB86	FASTA	593	66,914
	10 20 30 40 50 60 MAYDYALVHL KYTIPLAALL TVIAYPIFHR IHFLQIGSLI VVSFLATLPW DSYLIRSNIW 70 80 90 100 110 120 TYPPDAIIGP RLYGIPIEEL FFFVIQTYIT SLFYILLSKP LFHPLYLSTQ RNPPQRIARG 130 140 150 160 170 180 KVIGQGILVA LTLYGVHQIR TGGPGTYLGL ILAWAFPFAL LTFTVAGRFI LTLPLTSTVV 190 200 210 220 230 240 PIIIPTVYLW LVDELALGRG TWAIESGTKL GWCLFGVLDI EEATFFLATN ILIVFGMAVF 250 260 270 280 290 300 DQYLAIIFAF PHLFPKVPRS PTPLMLVQSR FSNTKQYDLE RIAGLSDAVT RLKAKSRSFY 310 320 330 340 350 360 LANSLFTGRL RIDLILLYSF CRLADDLVDD STSRTEVKSW TTKLYKFLDL HYKSDVKANK 370 380 390 400 410 420 ARINDYIDEA FPPEAKSALK YLPATILPSQ PLYQLIEGFE LDSQFSFHDS SESAKYPIVD 430 440 450 460 470 480 EDKLNYYGQC VAGTVGELCV ALIIEHCEPE MPDERKKMLM SVSRTMGVAL QYVNIARDIV 490 500 510 520 530 540 VDAEMGRVYL PTTWLKEEGL TPEDVVAHPR GKHVENLRRR LLSEAFKLYD EARPKMNGIP 550 560 570 580 590 KEARGPMIGA VETYMEIGRV LRELEGGVEL ERGKATVPGG RRLKTVLKAL FSA

References

[1]

"The genome sequence of the model ascomycete fungus Podospora anserina." Espagne E., Lespinet O., Malagnac F., Da Silva C., Jaillon O., Porcel B.M., Couloux A., Aury J.-M., Segurens B., Poulain J., Anthouard V., Grossetete S., Khalili H., Coppin E., Dequard-Chablat M., Picard M., Contamine V., Arnaise S. , Bourdais A., Berteaux-Lecellier V., Gautheret D., de Vries R.P., Battaglia E., Coutinho P.M., Danchin E.G.J., Henrissat B., El Khoury R., Sainsard-Chanet A., Boivin A., Pinan-Lucarre B., Sellem C.H., Debuchy R., Wincker P., Weissenbach J., Silar P. Genome Biol. 9:R77.1-R77.22(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S / ATCC MYA-4624 / DSM 980 / FGSC 10383.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU633899 Genomic DNA. Translation: CAP67633.1.
RefSeq	XP_001906962.1. XM_001906927.1.
3D structure databases
ProteinModelPortal	B2ATB0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6191495.
KEGG	pan:PODANSg3995.
Enzyme and pathway databases
UniPathway	UPA00799; UER00773. UPA00802.
Family and domain databases
Gene3D	1.10.600.10. 1 hit.
InterPro	IPR017825. Lycopene_cyclase_dom. IPR002060. Squ/phyt_synthse. IPR019845. Squalene/phytoene_synthase_CS. IPR008949. Terpenoid_synth. [Graphical view]
Pfam	PF00494. SQS_PSY. 1 hit. [Graphical view]
SUPFAM	SSF48576. Terpenoid_synth. 1 hit.
TIGRFAMs	TIGR03462. CarR_dom_SF. 2 hits.
PROSITE	PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LCPS_PODAN

Accession

Primary (citable) accession number: B2ATB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 31, 2011
Last sequence update:	May 20, 2008
Last modified:	March 6, 2013
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program