Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

B2AT43

- LIPA_PODAN

UniProt

B2AT43 - LIPA_PODAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Lipoyl synthase, mitochondrial

Gene

PODANS_1_14620

Organism
Podospora anserina (strain S / ATCC MYA-4624 / DSM 980 / FGSC 10383) (Pleurage anserina)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi140 – 1401Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi145 – 1451Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi151 – 1511Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi171 – 1711Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi175 – 1751Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi178 – 1781Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
Ordered Locus Names:PODANS_1_14620
OrganismiPodospora anserina (strain S / ATCC MYA-4624 / DSM 980 / FGSC 10383) (Pleurage anserina)
Taxonomic identifieri515849 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesLasiosphaeriaceaePodospora
ProteomesiUP000001197: Chromosome 1

Subcellular locationi

Mitochondrion UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2929MitochondrionUniRule annotationAdd
BLAST
Chaini30 – 426397Lipoyl synthase, mitochondrialPRO_0000398284Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliB2AT43.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

KOiK03644.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B2AT43-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASPAPLQRL QAPLRRSLAR AAVLSSRTYA TIPSPSDPGL TQSSPSPAAS
60 70 80 90 100
TTPAKKAPRP SYFKDTTVAS FSEFVGSQSA PLSLSEAYEI KTAEVGPAGR
110 120 130 140 150
KRTITRLPEW LKTPIPSSGA NPNFGKIKAD LRGLNLHTVC EEARCPNIGE
160 170 180 190 200
CWGGNDKSAA TATIMLMGDT CTRGCRFCSV KTNRKPPPLD PHEPENTAEA
210 220 230 240 250
LARWGLGYVV LTSVDRDDLA DGGARHFAET IRRIKQKKPT LLVEALTGDF
260 270 280 290 300
MGDLDMVKIV AESGLDVYAH NVETVEGLTP YVRDRRATFR QSLKVLEHVK
310 320 330 340 350
AVRGKEGIIT KTSIMLGLGE QEQEIWDTLR ELRKIDVDVV TFGQYMRPTK
360 370 380 390 400
RHLKVEKYVT PDEFDLWKQR ALDMGFLYCA SGPLVRSSYK AGEAFIENVL
410 420
RKRAGERAAA SASLDQVVAA EETKAL
Length:426
Mass (Da):46,688
Last modified:May 20, 2008 - v1
Checksum:i09B7F18A38D991A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU633899 Genomic DNA. Translation: CAP67566.1.
RefSeqiXP_001906895.1. XM_001906860.1.

Genome annotation databases

GeneIDi6191609.
KEGGipan:PODANSg3928.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU633899 Genomic DNA. Translation: CAP67566.1 .
RefSeqi XP_001906895.1. XM_001906860.1.

3D structure databases

ProteinModelPortali B2AT43.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 6191609.
KEGGi pan:PODANSg3928.

Phylogenomic databases

KOi K03644.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: S / ATCC MYA-4624 / DSM 980 / FGSC 10383.

Entry informationi

Entry nameiLIPA_PODAN
AccessioniPrimary (citable) accession number: B2AT43
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: May 20, 2008
Last modified: November 26, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3