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B2AT43

- LIPA_PODAN

UniProt

B2AT43 - LIPA_PODAN

Protein

Lipoyl synthase, mitochondrial

Gene

PODANS_1_14620

Organism
Podospora anserina (strain S / ATCC MYA-4624 / DSM 980 / FGSC 10383) (Pleurage anserina)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 1 (20 May 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi140 – 1401Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi145 – 1451Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi151 – 1511Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi171 – 1711Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi175 – 1751Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi178 – 1781Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lipoate synthaseUniRule annotation
    Short name:
    LSUniRule annotation
    Short name:
    Lip-synUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Gene namesi
    Ordered Locus Names:PODANS_1_14620
    OrganismiPodospora anserina (strain S / ATCC MYA-4624 / DSM 980 / FGSC 10383) (Pleurage anserina)
    Taxonomic identifieri515849 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesLasiosphaeriaceaePodospora
    ProteomesiUP000001197: Chromosome 1

    Subcellular locationi

    Mitochondrion UniRule annotation

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2929MitochondrionUniRule annotationAdd
    BLAST
    Chaini30 – 426397Lipoyl synthase, mitochondrialPRO_0000398284Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliB2AT43.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    KOiK03644.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B2AT43-1 [UniParc]FASTAAdd to Basket

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    MASPAPLQRL QAPLRRSLAR AAVLSSRTYA TIPSPSDPGL TQSSPSPAAS    50
    TTPAKKAPRP SYFKDTTVAS FSEFVGSQSA PLSLSEAYEI KTAEVGPAGR 100
    KRTITRLPEW LKTPIPSSGA NPNFGKIKAD LRGLNLHTVC EEARCPNIGE 150
    CWGGNDKSAA TATIMLMGDT CTRGCRFCSV KTNRKPPPLD PHEPENTAEA 200
    LARWGLGYVV LTSVDRDDLA DGGARHFAET IRRIKQKKPT LLVEALTGDF 250
    MGDLDMVKIV AESGLDVYAH NVETVEGLTP YVRDRRATFR QSLKVLEHVK 300
    AVRGKEGIIT KTSIMLGLGE QEQEIWDTLR ELRKIDVDVV TFGQYMRPTK 350
    RHLKVEKYVT PDEFDLWKQR ALDMGFLYCA SGPLVRSSYK AGEAFIENVL 400
    RKRAGERAAA SASLDQVVAA EETKAL 426
    Length:426
    Mass (Da):46,688
    Last modified:May 20, 2008 - v1
    Checksum:i09B7F18A38D991A2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU633899 Genomic DNA. Translation: CAP67566.1.
    RefSeqiXP_001906895.1. XM_001906860.1.

    Genome annotation databases

    GeneIDi6191609.
    KEGGipan:PODANSg3928.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU633899 Genomic DNA. Translation: CAP67566.1 .
    RefSeqi XP_001906895.1. XM_001906860.1.

    3D structure databases

    ProteinModelPortali B2AT43.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 6191609.
    KEGGi pan:PODANSg3928.

    Phylogenomic databases

    KOi K03644.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: S / ATCC MYA-4624 / DSM 980 / FGSC 10383.

    Entry informationi

    Entry nameiLIPA_PODAN
    AccessioniPrimary (citable) accession number: B2AT43
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: May 20, 2008
    Last modified: October 1, 2014
    This is version 40 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3