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B2AR91 (TRMB_PODAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
Transfer RNA methyltransferase 8
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:TRM8
Ordered Locus Names:PODANS_4_8250
OrganismPodospora anserina (strain S / ATCC MYA-4624 / DSM 980 / FGSC 10383) (Pleurage anserina) [Complete proteome]
Taxonomic identifier515849 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesLasiosphaeriaceaePodospora

Protein attributes

Sequence length284 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_03055

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_03055

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_03055

Subunit structure

Forms a complex with TRM82 By similarity. HAMAP-Rule MF_03055

Subcellular location

Nucleus By similarity HAMAP-Rule MF_03055.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentNucleus
   LigandRNA-binding
S-adenosyl-L-methionine
tRNA-binding
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 284284tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_03055
PRO_0000370604

Regions

Region125 – 1262S-adenosyl-L-methionine binding By similarity
Region160 – 1612S-adenosyl-L-methionine binding By similarity
Region258 – 2603S-adenosyl-L-methionine binding By similarity

Sites

Active site1831 By similarity
Binding site1021S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1801S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
B2AR91 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: 04BF84A9B7090516

FASTA28432,598
        10         20         30         40         50         60 
MAAKKNKKQK REDYRAAMKQ DDVLTMPRKK FYRQRAHANP FSDHQLVYPP HPDQMDWSTL 

        70         80         90        100        110        120 
YPAYVAQEES QPEATPSSTT EDLSAPVKVK KLTQDVEVAD IGCGFGGLLI ALAPVMPQTL 

       130        140        150        160        170        180 
VLGLEIRVSV TQFVEDRIKV LRRQNEESKA YQNVSVLRAN TMKFLPNFFR QGQLSKIFIC 

       190        200        210        220        230        240 
FPDPHFKARK HKQRIVSTTL NSEYAYAVRP GGVVYTITDV PDLHGWMVQH FEAHPMFERV 

       250        260        270        280 
GVEEQEGDPC VEIMRNATEE GKKVERNKGE KFVALFRRLE DPVF 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU633895 Genomic DNA. Translation: CAP66669.1.
RefSeqXP_001906003.1. XM_001905968.1.

3D structure databases

ProteinModelPortalB2AR91.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6190531.
KEGGpan:PODANSg3031.

Phylogenomic databases

KOK03439.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

HAMAPMF_03055. tRNA_methyltr_TrmB_euk.
InterProIPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_PODAN
AccessionPrimary (citable) accession number: B2AR91
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 20, 2008
Last modified: April 16, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways