B2AR91 (TRMB_PODAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
Transfer RNA methyltransferase 8
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

Name:	TRM8
ORF Names:	Pa_4_8250

Organism

Podospora anserina (strain S / ATCC MYA-4624 / DSM 980 / FGSC 10383) (Pleurage anserina) [Complete proteome]

Taxonomic identifier

515849 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Sordariomycetidae › Sordariales › Lasiosphaeriaceae › Podospora › Podospora anserina

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA.
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
Subunit structure	Forms a complex with TRM82 By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine tRNA-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	tRNA (guanine-N(7)-)-methyltransferase		PRO_0000370604
Regions
	Region	125 – 126	2	S-adenosyl-L-methionine binding By similarity
	Region	160 – 161	2	S-adenosyl-L-methionine binding By similarity
	Region	258 – 260	3	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	183	1	By similarity
	Binding site	102	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	180	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2AR91 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 04BF84A9B7090516	FASTA	284	32,598
	10 20 30 40 50 60 MAAKKNKKQK REDYRAAMKQ DDVLTMPRKK FYRQRAHANP FSDHQLVYPP HPDQMDWSTL 70 80 90 100 110 120 YPAYVAQEES QPEATPSSTT EDLSAPVKVK KLTQDVEVAD IGCGFGGLLI ALAPVMPQTL 130 140 150 160 170 180 VLGLEIRVSV TQFVEDRIKV LRRQNEESKA YQNVSVLRAN TMKFLPNFFR QGQLSKIFIC 190 200 210 220 230 240 FPDPHFKARK HKQRIVSTTL NSEYAYAVRP GGVVYTITDV PDLHGWMVQH FEAHPMFERV 250 260 270 280 GVEEQEGDPC VEIMRNATEE GKKVERNKGE KFVALFRRLE DPVF

References

[1]

"The genome sequence of the model ascomycete fungus Podospora anserina." Espagne E., Lespinet O., Malagnac F., Da Silva C., Jaillon O., Porcel B.M., Couloux A., Aury J.-M., Segurens B., Poulain J., Anthouard V., Grossetete S., Khalili H., Coppin E., Dequard-Chablat M., Picard M., Contamine V., Arnaise S. , Bourdais A., Berteaux-Lecellier V., Gautheret D., de Vries R.P., Battaglia E., Coutinho P.M., Danchin E.G.J., Henrissat B., El Khoury R., Sainsard-Chanet A., Boivin A., Pinan-Lucarre B., Sellem C.H., Debuchy R., Wincker P., Weissenbach J., Silar P. Genome Biol. 9:R77.1-R77.22(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S / ATCC MYA-4624 / DSM 980 / FGSC 10383.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU633895 Genomic DNA. Translation: CAP66669.1.
RefSeq	XP_001906003.1. XM_001905968.1.
3D structure databases
ProteinModelPortal	B2AR91.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6190531.
KEGG	pan:PODANSg3031.
Phylogenomic databases
KO	K03439.
Enzyme and pathway databases
UniPathway	UPA00989.
Family and domain databases
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. IPR025763. tRNA_(Gua-N-7)_MeTrfase_euk. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_PODAN

Accession

Primary (citable) accession number: B2AR91

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	May 20, 2008
Last modified:	October 3, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program