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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

TRM8

Organism
Podospora anserina (strain S / ATCC MYA-4624 / DSM 980 / FGSC 10383) (Pleurage anserina)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei102S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei180S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei183UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
Transfer RNA methyltransferase 8UniRule annotation
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:TRM8UniRule annotation
Ordered Locus Names:Pa_4_8250
ORF Names:PODANS_4_8250
OrganismiPodospora anserina (strain S / ATCC MYA-4624 / DSM 980 / FGSC 10383) (Pleurage anserina)
Taxonomic identifieri515849 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesLasiosphaeriaceaePodospora
Proteomesi
  • UP000001197 Componenti: Chromosome 4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003706041 – 284tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST284

Proteomic databases

PRIDEiB2AR91

Interactioni

Subunit structurei

Forms a complex with TRM82.UniRule annotation

Protein-protein interaction databases

STRINGi515849.XP_001906003.1

Structurei

3D structure databases

ProteinModelPortaliB2AR91
SMRiB2AR91
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 126S-adenosyl-L-methionine bindingUniRule annotation2
Regioni160 – 161S-adenosyl-L-methionine bindingUniRule annotation2
Regioni258 – 260S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3115 Eukaryota
COG0220 LUCA
KOiK03439
OrthoDBiEOG092C0GMH

Family and domain databases

HAMAPiMF_03055 tRNA_methyltr_TrmB_euk, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR025763 Trm8_euk
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
PTHR23417:SF16 PTHR23417:SF16, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

B2AR91-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAKKNKKQK REDYRAAMKQ DDVLTMPRKK FYRQRAHANP FSDHQLVYPP
60 70 80 90 100
HPDQMDWSTL YPAYVAQEES QPEATPSSTT EDLSAPVKVK KLTQDVEVAD
110 120 130 140 150
IGCGFGGLLI ALAPVMPQTL VLGLEIRVSV TQFVEDRIKV LRRQNEESKA
160 170 180 190 200
YQNVSVLRAN TMKFLPNFFR QGQLSKIFIC FPDPHFKARK HKQRIVSTTL
210 220 230 240 250
NSEYAYAVRP GGVVYTITDV PDLHGWMVQH FEAHPMFERV GVEEQEGDPC
260 270 280
VEIMRNATEE GKKVERNKGE KFVALFRRLE DPVF
Length:284
Mass (Da):32,598
Last modified:May 20, 2008 - v1
Checksum:i04BF84A9B7090516
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU633895 Genomic DNA Translation: CAP66669.1
FO904939 Genomic DNA Translation: CDP28405.1
RefSeqiXP_001906003.1, XM_001905968.1

Genome annotation databases

EnsemblFungiiCAP66669; CAP66669; PODANS_4_8250
GeneIDi6190531
KEGGipan:PODANSg3031

Similar proteinsi

Entry informationi

Entry nameiTRMB_PODAN
AccessioniPrimary (citable) accession number: B2AR91
Secondary accession number(s): A0A090CJP9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 20, 2008
Last modified: May 23, 2018
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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