B2AH98 (SYGA_CUPTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine--tRNA ligase alpha subunit
EC=6.1.1.14

Alternative name(s):
Glycyl-tRNA synthetase alpha subunit
Short name=GlyRS

Gene names

Name:	glyQ
Ordered Locus Names:	RALTA_A0479

Organism

Cupriavidus taiwanensis (strain R1 / LMG 19424) (Ralstonia taiwanensis (strain LMG 19424)) [Complete proteome] [HAMAP]

Taxonomic identifier

164546 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP MF_00254
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00254.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	Glycine--tRNA ligase alpha subunit HAMAP MF_00254		PRO_1000101180

Sequences

	Sequence		Length	Mass (Da)
	B2AH98 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: B5309D7791C5F771	FASTA	316	36,003
	10 20 30 40 50 60 MLTFQQMILT LQAYWDRQGC ALLQPIDLEV GAGTSHVHTF LRAIGPEPWR AAYVQPSRRP 70 80 90 100 110 120 KDGRYGENPN RLQHYYQYQV VLKPAPENIL VLYLGSLEAL GLDLKQNDIR FVEDDWENPT 130 140 150 160 170 180 LGAWGLGWEV WLNGMEVTQF TYFQQVGGID CKPITGEITY GIERLAMYLQ KVENVYDLVW 190 200 210 220 230 240 TEWVENGETR RLTYGDVYHQ NEVEQSTYNF EHSNTEILFR HFAEHEGEAK RLMGNGDGAD 250 260 270 280 290 300 AAAETGTRLA LPAYEQVLKA AHTFNLLDAR GAISVTERAA YIGRIRNLSR QVAQAYYDSR 310 EALGFPMCGQ RDGARA

References

[1]

"Genome sequence of the beta-rhizobium Cupriavidus taiwanensis and comparative genomics of rhizobia." Amadou C., Pascal G., Mangenot S., Glew M., Bontemps C., Capela D., Carrere S., Cruveiller S., Dossat C., Lajus A., Marchetti M., Poinsot V., Rouy Z., Servin B., Saad M., Schenowitz C., Barbe V., Batut J., Medigue C., Masson-Boivin C. Genome Res. 18:1472-1483(2008) [PubMed: 18490699] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: R1 / LMG 19424.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU633749 Genomic DNA. Translation: CAP63147.1.
RefSeq	YP_001795867.1. NC_010528.1.
3D structure databases
ProteinModelPortal	B2AH98.
SMR	B2AH98. Positions 2-302.
ModBase	Search...
Protein-protein interaction databases
STRING	B2AH98.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6301444.
GenomeReviews	Gene locus RALTA_A0479 in contig CU633749_GR.
KEGG	cti:RALTA_A0479.
PATRIC	21527604. VBICupTai42494_0486.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285316.
OMA	CRRPTDG.
ProtClustDB	PRK09348.
Family and domain databases
HAMAP	MF_00254. Gly_tRNA_synth_alpha. [Tree]
InterPro	IPR006194. Gly-tRNA-synth_II_heterodimer. IPR002310. Gly-tRNA_synth_IIc_asu. [Graphical view]
KO	K01878.
Pfam	PF02091. tRNA-synt_2e. 1 hit. [Graphical view]
PRINTS	PR01044. TRNASYNTHGA.
TIGRFAMs	TIGR00388. GlyQ. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGA_CUPTR

Accession

Primary (citable) accession number: B2AH98

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program