B2AGY8 (QUEF_CUPTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13

Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase

Gene names

Name:	queF
Ordered Locus Names:	RALTA_A0368

Organism

Cupriavidus taiwanensis (strain R1 / LMG 19424) (Ralstonia taiwanensis (strain LMG 19424)) [Complete proteome] [HAMAP]

Taxonomic identifier

164546 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP MF_00817
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00817
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00817
Subcellular location	Cytoplasm By similarity HAMAP MF_00817.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro preQ1 synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00817		PRO_1000134276

Sequences

	Sequence		Length	Mass (Da)
	B2AGY8 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 34C8A604C07289B1	FASTA	277	31,492
	10 20 30 40 50 60 MSLPEHSPLG KPSAYKTEYD ASLLFPIPRQ PKRTEIGLPE GKPVPFFGVD IWNAYELSWL 70 80 90 100 110 120 NLKGKPQVAL ATFIIPSDTP NIVESKSFKL YLNSFNQTRI ASPEALQQLL HHDLSEATGG 130 140 150 160 170 180 TVQVRLVTEA DLGKQQMGEL DGLLLDRLDI EVDRYEPAPE LLFADQDETP VEETLVSHLL 190 200 210 220 230 240 KSNCLVTGQP DWGSVQIRYV GAPINQEALL KYLISFRNHN EFHEQCVERI FMDVMRQCKP 250 260 270 VKLAVYARYT RRGGLDINPF RTNFNTAWPD NKRNARQ

References

[1]

"Genome sequence of the beta-rhizobium Cupriavidus taiwanensis and comparative genomics of rhizobia." Amadou C., Pascal G., Mangenot S., Glew M., Bontemps C., Capela D., Carrere S., Cruveiller S., Dossat C., Lajus A., Marchetti M., Poinsot V., Rouy Z., Servin B., Saad M., Schenowitz C., Barbe V., Batut J., Medigue C., Masson-Boivin C. Genome Res. 18:1472-1483(2008) [PubMed: 18490699] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: R1 / LMG 19424.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU633749 Genomic DNA. Translation: CAP63037.1.
RefSeq	YP_001795759.1. NC_010528.1.
3D structure databases
ProteinModelPortal	B2AGY8.
SMR	B2AGY8. Positions 15-277.
ModBase	Search...
Protein-protein interaction databases
STRING	B2AGY8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6301363.
GenomeReviews	Gene locus RALTA_A0368 in contig CU633749_GR.
KEGG	cti:RALTA_A0368.
PATRIC	21527374. VBICupTai42494_0371.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG289139.
OMA	NPFRTSH.
ProtClustDB	PRK11792.
Family and domain databases
HAMAP	MF_00817. QueF_type2. [Tree]
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
KO	K06879.
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMs	TIGR03138. QueF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_CUPTR

Accession

Primary (citable) accession number: B2AGY8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program