B2AGT9 (ISPE_CUPTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	RALTA_A0318

Organism

Cupriavidus taiwanensis (strain R1 / LMG 19424) (Ralstonia taiwanensis (strain LMG 19424)) [Complete proteome] [HAMAP]

Taxonomic identifier

164546 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP-Rule MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP-Rule MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP-Rule MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: UniProtKB-UniPathway terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP-Rule MF_00061		PRO_1000092081
Regions
	Nucleotide binding	103 – 113	11	ATP Potential
Sites
	Active site	20	1	By similarity
	Active site	145	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2AGT9 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: CC4EFB8D35186C0A	FASTA	292	31,640
	10 20 30 40 50 60 MPSSHPLPPP ELRDCPAPAK LNLFLHVTGR RADGYHTLQT VFQLVDWCDM LHFRRRDDGV 70 80 90 100 110 120 VARVTEVPGV PAESDLVVRA ARALQAATGT TFGAEIAIDK VLPMGGGIGG GSSDAATTLL 130 140 150 160 170 180 ALNHLWGLGL TRPELMRIGL TLGADVPVFV LGQNAFAQGI GEELTPVELP DSWFVIIHPK 190 200 210 220 230 240 QHVPTAEIFS DECLTRDTPV SIIAVFAART NKFDFGRNDL EPIATAKFGE VARALAWLKQ 250 260 270 280 290 HNQHARMTGS GACVFARFPD AKTAQQVLER LPPEWDGRCV RSLAHHPLAA FA

References

[1]

"Genome sequence of the beta-rhizobium Cupriavidus taiwanensis and comparative genomics of rhizobia." Amadou C., Pascal G., Mangenot S., Glew M., Bontemps C., Capela D., Carrere S., Cruveiller S., Dossat C., Lajus A., Marchetti M., Poinsot V., Rouy Z., Servin B., Saad M., Schenowitz C., Barbe V., Batut J., Medigue C., Masson-Boivin C. Genome Res. 18:1472-1483(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: R1 / LMG 19424.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU633749 Genomic DNA. Translation: CAP62988.1.
RefSeq	YP_001795710.1. NC_010528.1.
3D structure databases
ProteinModelPortal	B2AGT9.
ModBase	Search...
Protein-protein interaction databases
STRING	164546.RALTA_A0318.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAP62988; CAP62988; RALTA_A0318.
GeneID	6301656.
KEGG	cti:RALTA_A0318.
PATRIC	21527272. VBICupTai42494_0320.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HOG000019601.
KO	K00919.
OMA	TGACVFG.
ProtClustDB	PRK00343.
Enzyme and pathway databases
BioCyc	CTAI164546:GJNE-318-MONOMER.
UniPathway	UPA00056; UER00094.
Family and domain databases
Gene3D	3.30.230.10. 1 hit.
HAMAP	MF_00061. IspE.
InterPro	IPR013750. GHMP_kinase_C_dom. IPR006204. GHMP_kinase_N_dom. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
PANTHER	PTHR20861:SF2. PTHR20861:SF2. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. ispE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_CUPTR

Accession

Primary (citable) accession number: B2AGT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 20, 2008
Last modified:	May 1, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program