Reviewed, UniProtKB/Swiss-Prot B2AG85 (ACEK_CUPTR)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase kinase/phosphatase
      Short name=IDH kinase/phosphatase
      Short name=IDHK/P
    EC=2.7.11.5
    EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	RALTA_A0111

Organism

Cupriavidus taiwanensis (strain R1 / LMG 19424) (Ralstonia taiwanensis (strain LMG 19424)) [Complete proteome] [HAMAP]

Taxonomic identifier

164546 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	615 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity.
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: HAMAP phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 615	615	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_1000133263
Regions
	Nucleotide binding	328 – 334	7	ATP By similarity
Sites
	Active site	384	1	By similarity
	Binding site	349	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2AG85-1 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 9F7A057EBC4994F1	FASTA	615	71,671
	10 20 30 40 50 60 MSHFPKLLSS QIAYDVARTM LDGFDKHYRL FREVSHQAKL KFEAGDWHGL QQIQRDRIAF 70 80 90 100 110 120 YNERVRESSV ILEDEYDAEN IEDEIWQQIK LHYIGLLTNH HQPELAETFF NSVCTRILHR 130 140 150 160 170 180 SYFNNDFIFV RPAISTEYIE NEESPTRPTF RAYYPGSREG MAACFERIVH NFQLERPFED 190 200 210 220 230 240 LQRDIGYVVR AVGEHFGDLR IAPNFQIHTL SSLFFRNKAA FIIGRILNGD RTFPLAIPIL 250 260 270 280 290 300 HGPSGKLVLD TVLLKKEQLL ILFSFTHSYF MVDMEIPSAY VTFLRDIMPR KPRAEIYTSL 310 320 330 340 350 360 GLQKQGKNLF YRDFLHHLQH SSDKFIVAPG IRGLVMLVFT LPSYPYVFKV IRDVFPAPKE 370 380 390 400 410 420 TTRELVKSKY QLVKQHDRVG RMADTLEYSD VAFPLSRFDE ALVREFEQHA PSMIEYQRAK 430 440 450 460 470 480 DGGEEIVVRH VYIERRMTPL NIYLQEGSDA QVEHGVIEYG NAIKELIAAN IFPGDMLYKN 490 500 510 520 530 540 FGVTRHGRVV FYDYDEIEYL TDCNIRHVPQ PRNEEEEMSG EVWYTVRPHD IFPETFRTFL 550 560 570 580 590 600 LGDPRVGAAF LRHHADFFDP AMWQSHKDRL LAGHVHDFFA YHASDRFIHR YGAAAEPPAT 610 PPVKQPDAGP ARRVA

References

[1]

"Genome sequence of the beta-rhizobium Cupriavidus taiwanensis and comparative genomics of rhizobia." Amadou C., Pascal G., Mangenot S., Glew M., Bontemps C., Capela D., Carrere S., Cruveiller S., Dossat C., Lajus A., Marchetti M., Poinsot V., Rouy Z., Servin B., Saad M., Schenowitz C., Barbe V., Batut J., Medigue C., Masson-Boivin C. Genome Res. 18:1472-1483(2008) [PubMed: 18490699] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU633749 Genomic DNA. Translation: CAP62784.1.
RefSeq	YP_001795506.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6301788.
GenomeReviews	Gene locus RALTA_A0111 in contig CU633749_GR.
KEGG	cti:RALTA_A0111.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	FYRDFLH.
Family and domain databases
HAMAP	MF_00747. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACEK_CUPTR

Accession

Primary (citable) accession number: B2AG85

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	May 20, 2008
Last modified:	November 3, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)