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B2AG37

- LIPA_CUPTR

UniProt

B2AG37 - LIPA_CUPTR

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Protein

Lipoyl synthase

Gene
lipA, RALTA_A0063
Organism
Cupriavidus taiwanensis (strain R1 / LMG 19424) (Ralstonia taiwanensis (strain LMG 19424))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi81 – 811Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi86 – 861Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi92 – 921Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi107 – 1071Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi111 – 1111Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi114 – 1141Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCTAI164546:GJNE-63-MONOMER.
CTAI977880:GLC7-63-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:RALTA_A0063
OrganismiCupriavidus taiwanensis (strain R1 / LMG 19424) (Ralstonia taiwanensis (strain LMG 19424))
Taxonomic identifieri164546 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
ProteomesiUP000001692: Chromosome 1

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 334334Lipoyl synthaseUniRule annotationPRO_1000099599Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi164546.RALTA_A0063.

Structurei

3D structure databases

ProteinModelPortaliB2AG37.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiFERIATN.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

B2AG37-1 [UniParc]FASTAAdd to Basket

« Hide

MSDALIAPNA SSSEAPQSPA EHYDPTRKQK SADKTARIPI KIVPAEKLKK    50
PDWIRVKAAT GNSRFYEIKD ILRANNLVTV CEEASCPNIG ECFGKGTATF 100
MIMGDKCTRR CPFCDVGHGR PDPLDVNEPG NLARTIAQLK LNYVVITSVD 150
RDDLRDGGAQ HYVDCISQTR ELSPATRIEV LVPDFRGRLD KALDILQACP 200
PDVMNHNMET VPRLYKQARP GADYAHSLKL LQEFKRRNPN VPTKSGLMVG 250
LGETDEEILE VMRDMRAHDI DMLTIGQYLA PSNHHLPVLR YVHPDTFKMF 300
EEEAYKMGFT HAAVGAMVRS SYHADQQAHQ AGFA 334
Length:334
Mass (Da):37,271
Last modified:May 20, 2008 - v1
Checksum:i877886C8BA1D635F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU633749 Genomic DNA. Translation: CAP62736.1.
RefSeqiYP_001795458.1. NC_010528.1.

Genome annotation databases

EnsemblBacteriaiCAP62736; CAP62736; RALTA_A0063.
GeneIDi6301317.
KEGGicti:RALTA_A0063.
PATRICi21526756. VBICupTai42494_0064.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU633749 Genomic DNA. Translation: CAP62736.1 .
RefSeqi YP_001795458.1. NC_010528.1.

3D structure databases

ProteinModelPortali B2AG37.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 164546.RALTA_A0063.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAP62736 ; CAP62736 ; RALTA_A0063 .
GeneIDi 6301317.
KEGGi cti:RALTA_A0063.
PATRICi 21526756. VBICupTai42494_0064.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi FERIATN.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci CTAI164546:GJNE-63-MONOMER.
CTAI977880:GLC7-63-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: R1 / LMG 19424.

Entry informationi

Entry nameiLIPA_CUPTR
AccessioniPrimary (citable) accession number: B2AG37
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 20, 2008
Last modified: May 14, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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