Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Acetate kinase

Gene

ackA

Organism
Natranaerobius thermophilus (strain ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction.UniRule annotation

Catalytic activityi

ATP + acetate = ADP + acetyl phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Mg2+. Can also accept Mn2+.UniRule annotation

Pathwayi: acetyl-CoA biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes acetyl-CoA from acetate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Acetate kinase (ackA)
  2. no protein annotated in this organism
This subpathway is part of the pathway acetyl-CoA biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetyl-CoA from acetate, the pathway acetyl-CoA biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi7MagnesiumUniRule annotation1
Binding sitei14ATPUniRule annotation1
Binding sitei91SubstrateUniRule annotation1
Active sitei148Proton donor/acceptorUniRule annotation1
Sitei180Transition state stabilizerUniRule annotation1
Sitei241Transition state stabilizerUniRule annotation1
Metal bindingi384MagnesiumUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi208 – 212ATPUniRule annotation5
Nucleotide bindingi283 – 285ATPUniRule annotation3
Nucleotide bindingi331 – 335ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00340; UER00458.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetate kinaseUniRule annotation (EC:2.7.2.1UniRule annotation)
Alternative name(s):
AcetokinaseUniRule annotation
Gene namesi
Name:ackAUniRule annotation
Ordered Locus Names:Nther_2303
OrganismiNatranaerobius thermophilus (strain ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF)
Taxonomic identifieri457570 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaNatranaerobialesNatranaerobiaceaeNatranaerobius
Proteomesi
  • UP000001683 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000899851 – 398Acetate kinaseAdd BLAST398

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi457570.Nther_2303.

Structurei

3D structure databases

ProteinModelPortaliB2A8I5.
SMRiB2A8I5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the acetokinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C6H. Bacteria.
COG0282. LUCA.
HOGENOMiHOG000288399.
KOiK00925.
OMAiWKALTGT.
OrthoDBiPOG091H02K4.

Family and domain databases

HAMAPiMF_00020. Acetate_kinase. 1 hit.
InterProiView protein in InterPro
IPR004372. Ac/propionate_kinase.
IPR000890. Aliphatic_acid_kin_short-chain.
IPR023865. Aliphatic_acid_kinase_CS.
PANTHERiPTHR21060. PTHR21060. 1 hit.
PfamiView protein in Pfam
PF00871. Acetate_kinase. 1 hit.
PIRSFiPIRSF000722. Acetate_prop_kin. 1 hit.
PRINTSiPR00471. ACETATEKNASE.
TIGRFAMsiTIGR00016. ackA. 1 hit.
PROSITEiView protein in PROSITE
PS01075. ACETATE_KINASE_1. 1 hit.
PS01076. ACETATE_KINASE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

B2A8I5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKILVINCGS SSLKYQVFNM ENEDVLAKGA IERIGIEGSF LKHEPAGGEE
60 70 80 90 100
VKIEKDVTDH KAAFKMVVDA ITDEGHGVLK DLKEIEAVGH RVVHGGEEFS
110 120 130 140 150
GSVIIDDDVM NALESCINLA PLHNPPNIYG IEASKANMPG VPQVGVFDTA
160 170 180 190 200
FHQTMPKKAY LYGIPYEYYE KHSVRRYGFH GTSHKYVASR AADMLGKPLE
210 220 230 240 250
DLKLITCHLG NGASVAAINK GESVDTSMGL TPLEGLVMGT RSGSMDPSII
260 270 280 290 300
KFLMDKEGLS IDEVDNILNK KSGVLGLSGL SNDFRDLEEA AAEGNERAEN
310 320 330 340 350
TLEVFYYTVS KYIGSYLAAL NGADAIIFTA GLGENSPSVR EAVTENLSYA
360 370 380 390
GIKVDKDKNQ VRGKETDIST DDASTRVLVV PTNEELMIAR EAKGLLGK
Length:398
Mass (Da):43,048
Last modified:May 20, 2008 - v1
Checksum:i76B9B176F4B0AC00
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001034 Genomic DNA. Translation: ACB85869.1.
RefSeqiWP_012448719.1. NC_010718.1.

Genome annotation databases

EnsemblBacteriaiACB85869; ACB85869; Nther_2303.
KEGGinth:Nther_2303.

Similar proteinsi

Entry informationi

Entry nameiACKA_NATTJ
AccessioniPrimary (citable) accession number: B2A8I5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 20, 2008
Last modified: June 7, 2017
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families