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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Natranaerobius thermophilus (strain ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei589 – 5891ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciNTHE457570:GHRL-563-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Nther_0537
OrganismiNatranaerobius thermophilus (strain ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF)
Taxonomic identifieri457570 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaNatranaerobialesNatranaerobiaceaeNatranaerobius
ProteomesiUP000001683 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 826826Leucine--tRNA ligasePRO_1000091337Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi457570.Nther_0537.

Structurei

3D structure databases

ProteinModelPortaliB2A6C2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi41 – 5111"HIGH" regionAdd
BLAST
Motifi586 – 5905"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2A6C2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEYQPQKIE SKWQKRWQDE ISYETEVDHN KDKYYVLEMF PYPSGKLHMG
60 70 80 90 100
HMRVYSIGDV LARFQRMRGY NVLHPMGWDA FGLPAENAAI ENQDLPSRWT
110 120 130 140 150
YANIDNMKDQ LQALGTSYDW KREVSTCSPD YYKWTQWMFL QLYKNGLAYK
160 170 180 190 200
KKAPVNWCPE CETVLANEQV ENGACWRCDS MVEQKQLSQW FFKITEYADR
210 220 230 240 250
LDQDLELLEE WPDRVKTMQK NWIGKSQGTE IDFPVKGSRE KIRAFTTRPD
260 270 280 290 300
TIFGATYMVL APEHPMTEQL VQGTDQEKEV MDFIKGVHEM GKEAREAEDL
310 320 330 340 350
EKEGVFTGRW AINPLNGEEI PILVGNYVLM EYGTGAIMAV PAHDQRDFQF
360 370 380 390 400
AHKYNLPIKE VVTPPEGEGK TESETHDELK KAYTDHGILI NSPGYNGMTS
410 420 430 440 450
EQAIEQITRD IENKGIGAGV TTYRLRDWLV SRQRYWGAPI PVLYCDQCGI
460 470 480 490 500
LPVPEDELPV QLPEDVDFSQ GSRNVLAARQ DFVETSCPQC GGSAQRETDT
510 520 530 540 550
MDTFVCSSWY FLRYTTPWDN EVPFRQEDVN YWMPVDQYIG GIEHAVLHLL
560 570 580 590 600
YARFFTKVMY DQGYTNFKEP FSRLLAQGMV NKDGAKMSKS KGNVVSPDEI
610 620 630 640 650
LRTYGADTGR LFILFAAPPE KDLDWNDEGV EGCYRFLQRL YRLVNDNQNL
660 670 680 690 700
VDLQLDDTKF TKEDKEYHRL IHKTIKKVTD DISERHNFNT AISAIMELTN
710 720 730 740 750
ASSKYKEQKE VNESLLRTGL ETIVMLLAPF TPHIAEELWE TLGYQDSVHK
760 770 780 790 800
LNWPSYDEKA MVAEEAEMVV QVNGKVRDHL TVPADSSEET IKEKALEREK
810 820
VQKYISGAEI KKVIVIPQKL VNIVCK
Length:826
Mass (Da):95,481
Last modified:May 20, 2008 - v1
Checksum:i7FE2F0098DB072CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001034 Genomic DNA. Translation: ACB84133.1.
RefSeqiYP_001916721.1. NC_010718.1.

Genome annotation databases

EnsemblBacteriaiACB84133; ACB84133; Nther_0537.
KEGGinth:Nther_0537.
PATRICi22669694. VBINatThe92436_0557.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001034 Genomic DNA. Translation: ACB84133.1.
RefSeqiYP_001916721.1. NC_010718.1.

3D structure databases

ProteinModelPortaliB2A6C2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi457570.Nther_0537.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACB84133; ACB84133; Nther_0537.
KEGGinth:Nther_0537.
PATRICi22669694. VBINatThe92436_0557.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciNTHE457570:GHRL-563-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome of Natranaerobius thermophilus JW/NM-WN-LF."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.
    , Kyrpides N., Lykidis A., Mesbah N.M., Wiegel J.
    Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF.

Entry informationi

Entry nameiSYL_NATTJ
AccessioniPrimary (citable) accession number: B2A6C2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 20, 2008
Last modified: April 1, 2015
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.