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B2A6C2 (SYL_NATTJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Nther_0537
OrganismNatranaerobius thermophilus (strain ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF) [Complete proteome] [HAMAP]
Taxonomic identifier457570 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaNatranaerobialesNatranaerobiaceaeNatranaerobius

Protein attributes

Sequence length826 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 826826Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091337

Regions

Motif41 – 5111"HIGH" region HAMAP-Rule MF_00049
Motif586 – 5905"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5891ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B2A6C2 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: 7FE2F0098DB072CC

FASTA82695,481
        10         20         30         40         50         60 
MKEYQPQKIE SKWQKRWQDE ISYETEVDHN KDKYYVLEMF PYPSGKLHMG HMRVYSIGDV 

        70         80         90        100        110        120 
LARFQRMRGY NVLHPMGWDA FGLPAENAAI ENQDLPSRWT YANIDNMKDQ LQALGTSYDW 

       130        140        150        160        170        180 
KREVSTCSPD YYKWTQWMFL QLYKNGLAYK KKAPVNWCPE CETVLANEQV ENGACWRCDS 

       190        200        210        220        230        240 
MVEQKQLSQW FFKITEYADR LDQDLELLEE WPDRVKTMQK NWIGKSQGTE IDFPVKGSRE 

       250        260        270        280        290        300 
KIRAFTTRPD TIFGATYMVL APEHPMTEQL VQGTDQEKEV MDFIKGVHEM GKEAREAEDL 

       310        320        330        340        350        360 
EKEGVFTGRW AINPLNGEEI PILVGNYVLM EYGTGAIMAV PAHDQRDFQF AHKYNLPIKE 

       370        380        390        400        410        420 
VVTPPEGEGK TESETHDELK KAYTDHGILI NSPGYNGMTS EQAIEQITRD IENKGIGAGV 

       430        440        450        460        470        480 
TTYRLRDWLV SRQRYWGAPI PVLYCDQCGI LPVPEDELPV QLPEDVDFSQ GSRNVLAARQ 

       490        500        510        520        530        540 
DFVETSCPQC GGSAQRETDT MDTFVCSSWY FLRYTTPWDN EVPFRQEDVN YWMPVDQYIG 

       550        560        570        580        590        600 
GIEHAVLHLL YARFFTKVMY DQGYTNFKEP FSRLLAQGMV NKDGAKMSKS KGNVVSPDEI 

       610        620        630        640        650        660 
LRTYGADTGR LFILFAAPPE KDLDWNDEGV EGCYRFLQRL YRLVNDNQNL VDLQLDDTKF 

       670        680        690        700        710        720 
TKEDKEYHRL IHKTIKKVTD DISERHNFNT AISAIMELTN ASSKYKEQKE VNESLLRTGL 

       730        740        750        760        770        780 
ETIVMLLAPF TPHIAEELWE TLGYQDSVHK LNWPSYDEKA MVAEEAEMVV QVNGKVRDHL 

       790        800        810        820 
TVPADSSEET IKEKALEREK VQKYISGAEI KKVIVIPQKL VNIVCK 

« Hide

References

[1]"Complete sequence of chromosome of Natranaerobius thermophilus JW/NM-WN-LF."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Lykidis A., Mesbah N.M., Wiegel J.
Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001034 Genomic DNA. Translation: ACB84133.1.
RefSeqYP_001916721.1. NC_010718.1.

3D structure databases

ProteinModelPortalB2A6C2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING457570.Nther_0537.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACB84133; ACB84133; Nther_0537.
GeneID6314264.
KEGGnth:Nther_0537.
PATRIC22669694. VBINatThe92436_0557.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycNTHE457570:GHRL-563-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_NATTJ
AccessionPrimary (citable) accession number: B2A6C2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 20, 2008
Last modified: April 16, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries