B2A5S2 (MTNA_NATTJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methylthioribose-1-phosphate isomerase
Short name=M1Pi
Short name=MTR-1-P isomerase
EC=5.3.1.23

Alternative name(s):
S-methyl-5-thioribose-1-phosphate isomerase

Gene names

Name:	mtnA
Ordered Locus Names:	Nther_0419

Organism

Natranaerobius thermophilus (strain ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF) [Complete proteome] [HAMAP]

Taxonomic identifier

457570 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Natranaerobiales › Natranaerobiaceae › Natranaerobius › Natranaerobius thermophilus

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P) By similarity. HAMAP-Rule MF_01678
Catalytic activity	S-methyl-5-thio-alpha-D-ribose 1-phosphate = S-methyl-5-thio-D-ribulose 1-phosphate. HAMAP-Rule MF_01678
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 1/6. HAMAP-Rule MF_01678
Sequence similarities	Belongs to the eIF-2B alpha/beta/delta subunits family. MtnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-methionine biosynthetic process from S-adenosylmethionine Inferred from electronic annotation. Source: HAMAP L-methionine salvage from methylthioadenosine Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	S-methyl-5-thioribose-1-phosphate isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Methylthioribose-1-phosphate isomerase HAMAP-Rule MF_01678		PRO_0000357209
Regions
	Region	47 – 49	3	Substrate binding By similarity
	Region	250 – 251	2	Substrate binding By similarity
Sites
	Active site	240	1	Proton donor By similarity
	Binding site	90	1	Substrate By similarity
	Binding site	199	1	Substrate By similarity
	Site	160	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2A5S2 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: CF4B02B6463FA76D	FASTA	347	38,588
	10 20 30 40 50 60 MVSVKPLKFE DDELLLLDQR KLPGKEEYFT CKTYQDVHFA IKEMVCRGAP LIGAVGAYGV 70 80 90 100 110 120 ALACREFINE SQEKFQQETK RAISELSSAR PTAVNLFWAL NKMNKLLGQV MEENTAPEDI 130 140 150 160 170 180 YPIILNEAHK ISDQELQRNY RIAEFGDQVI SNGDKILTHC NTGALATTGY GTALGVIRQA 190 200 210 220 230 240 HYNHKDIFVY VDETRPRLQG AKLTAWELKQ EQLPFALIPD SSAAVLIKNG DIDVIFVGAD 250 260 270 280 290 300 RIASNGDTAN KIGTFMLSIL AKKYKVPFYV VAPVSTIDFQ AKNGDDITIE ERSSKEVTEI 310 320 330 340 DGVRVAPTDI QVYNPAFDIT PAENITGIIT EKGIIGPNNR EIMTIQE

References

[1]

"Complete sequence of chromosome of Natranaerobius thermophilus JW/NM-WN-LF." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Lykidis A., Mesbah N.M., Wiegel J. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001034 Genomic DNA. Translation: ACB84015.1.
RefSeq	YP_001916603.1. NC_010718.1.
3D structure databases
ProteinModelPortal	B2A5S2.
SMR	B2A5S2. Positions 3-341.
ModBase	Search...
Protein-protein interaction databases
STRING	457570.Nther_0419.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACB84015; ACB84015; Nther_0419.
GeneID	6317113.
KEGG	nth:Nther_0419.
PATRIC	22669460. VBINatThe92436_0440.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0182.
HOGENOM	HOG000224730.
KO	K08963.
OMA	HGVPHTV.
Enzyme and pathway databases
BioCyc	NTHE457570:GHRL-445-MONOMER.
UniPathway	UPA00904; UER00874.
Family and domain databases
Gene3D	1.20.120.420. 1 hit.
HAMAP	MF_01678. Salvage_MtnA.
InterPro	IPR000649. IF-2B-related. IPR005251. IF-2BI_MTNA. IPR011559. Initiation_fac_2B_a/b/d. IPR027363. M1Pi_N. [Graphical view]
PANTHER	PTHR10233. PTHR10233. 1 hit. PTHR10233:SF6. PTHR10233:SF6. 1 hit.
Pfam	PF01008. IF-2B. 1 hit. [Graphical view]
TIGRFAMs	TIGR00524. eIF-2B_rel. 1 hit. TIGR00512. salvage_mtnA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTNA_NATTJ

Accession

Primary (citable) accession number: B2A5S2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	May 20, 2008
Last modified:	May 29, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program