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B2A5L6 (RUVC_NATTJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RuvC

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene names
Name:ruvC
Ordered Locus Names:Nther_1798
OrganismNatranaerobius thermophilus (strain ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF) [Complete proteome] [HAMAP]
Taxonomic identifier457570 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaNatranaerobialesNatranaerobiaceaeNatranaerobius

Protein attributes

Sequence length161 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00034

Sequence similarities

Belongs to the RuvC family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 161161Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034
PRO_1000090540

Sites

Metal binding71Magnesium By similarity
Metal binding671Magnesium By similarity
Metal binding1401Magnesium By similarity
Metal binding1431Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
B2A5L6 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: 6DA4BB09D79FA099

FASTA16117,370
        10         20         30         40         50         60 
MIIMGLDPGI AACGYGIIHA KGTKLECLAC GVLRTYSDES ATKRLESIYQ GVSNLISEFS 

        70         80         90        100        110        120 
PNTVAVESLF FAKNSKTAMK VGQAKGVLML AAAHNNLEIF EYTPLQVKQG VCGYGSASKE 

       130        140        150        160 
QVQKMVKQIL SLDKIPKPDD AADALAVSLC HSQAYRLRTR L 

« Hide

References

[1]"Complete sequence of chromosome of Natranaerobius thermophilus JW/NM-WN-LF."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Lykidis A., Mesbah N.M., Wiegel J.
Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001034 Genomic DNA. Translation: ACB85370.1.
RefSeqYP_001917958.1. NC_010718.1.

3D structure databases

ProteinModelPortalB2A5L6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING457570.Nther_1798.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACB85370; ACB85370; Nther_1798.
GeneID6315261.
KEGGnth:Nther_1798.
PATRIC22672292. VBINatThe92436_1843.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0817.
HOGENOMHOG000012182.
KOK01159.
OMAYTALQMK.
OrthoDBEOG6RG044.

Enzyme and pathway databases

BioCycNTHE457570:GHRL-1838-MONOMER.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
HAMAPMF_00034. RuvC.
InterProIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSPR00696. RSOLVASERUVC.
SUPFAMSSF53098. SSF53098. 1 hit.
TIGRFAMsTIGR00228. ruvC. 1 hit.
PROSITEPS01321. RUVC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRUVC_NATTJ
AccessionPrimary (citable) accession number: B2A5L6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 20, 2008
Last modified: April 16, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families