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B2A3U7 (B2A3U7_NATTJ) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Riboflavin biosynthesis protein RibBA HAMAP-Rule MF_01283
Gene names
Name:ribBA HAMAP-Rule MF_01283
Ordered Locus Names:Nther_0124 EMBL ACB83723.1
OrganismNatranaerobius thermophilus (strain ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF) [Complete proteome] [HAMAP] EMBL ACB83723.1
Taxonomic identifier457570 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Natranaerobiales › Natranaerobiaceae › Natranaerobius ›

Protein attributes

Sequence length412 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate By similarity. HAMAP-Rule MF_01283 SAAS SAAS017945

Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate By similarity. HAMAP-Rule MF_01283 SAAS SAAS017945

Catalytic activity

D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate. HAMAP-Rule MF_01283 SAAS SAAS017945

GTP + 3 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate. HAMAP-Rule MF_01283 SAAS SAAS017945

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_01283 SAAS SAAS017945

Binds 2 divalent metal cations per subunit. Magnesium or manganese By similarity. HAMAP-Rule MF_01283 SAAS SAAS017945

Pathway

Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1. HAMAP-Rule MF_01283 SAAS SAAS017945

Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 1/4. HAMAP-Rule MF_01283 SAAS SAAS017945

Sequence similarities

In the C-terminal section; belongs to the GTP cyclohydrolase II family. HAMAP-Rule MF_01283

In the N-terminal section; belongs to the DHBP synthase family. HAMAP-Rule MF_01283

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding252 – 2565GTP By similarity HAMAP-Rule MF_01283
Nucleotide binding295 – 2973GTP By similarity HAMAP-Rule MF_01283
Region1 – 200200DHBP synthase By similarity HAMAP-Rule MF_01283
Region27 – 282D-ribulose 5-phosphate binding By similarity HAMAP-Rule MF_01283
Region139 – 1435D-ribulose 5-phosphate binding By similarity HAMAP-Rule MF_01283
Region201 – 412212GTP cyclohydrolase II By similarity HAMAP-Rule MF_01283

Sites

Active site3291Proton acceptor; for GTP cyclohydrolase activity By similarity HAMAP-Rule MF_01283
Active site3311Nucleophile; for GTP cyclohydrolase activity By similarity HAMAP-Rule MF_01283
Metal binding281Magnesium or manganese 1 By similarity HAMAP-Rule MF_01283
Metal binding281Magnesium or manganese 2 By similarity HAMAP-Rule MF_01283
Metal binding1421Magnesium or manganese 2 By similarity HAMAP-Rule MF_01283
Metal binding2571Zinc; catalytic By similarity HAMAP-Rule MF_01283
Metal binding2681Zinc; catalytic By similarity HAMAP-Rule MF_01283
Metal binding2701Zinc; catalytic By similarity HAMAP-Rule MF_01283
Binding site321D-ribulose 5-phosphate By similarity HAMAP-Rule MF_01283
Binding site1631D-ribulose 5-phosphate By similarity HAMAP-Rule MF_01283
Binding site2731GTP By similarity HAMAP-Rule MF_01283
Binding site3171GTP By similarity HAMAP-Rule MF_01283
Binding site3521GTP By similarity HAMAP-Rule MF_01283
Binding site3571GTP By similarity HAMAP-Rule MF_01283
Site1251Essential for DHBP synthase activity By similarity HAMAP-Rule MF_01283
Site1631Essential for DHBP synthase activity By similarity HAMAP-Rule MF_01283

Sequences

Sequence LengthMass (Da)Tools
B2A3U7 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: 2AF5975E3E0074B2

FASTA41245,877
        10         20         30         40         50         60 
MTLSTIKEAL EDLKNGKMVI VVDDEDRENE GDLVIPAEKA TPQAVNFMAK YGRGLICMPM 

        70         80         90        100        110        120 
KEERLQELDI PLMVNDNTDE MGTAFTVSVD YKDCHTGISA YERAMTVTKL IKEDSKPEDF 

       130        140        150        160        170        180 
NRPGHIFPLA ARQGGVLKRA GHTEAAIDLA YLAGCYPAGV ICEIMSDDGT MARLPELEQF 

       190        200        210        220        230        240 
AREHDLKIIS IADLISYRRN SEQLIEAVAD INLPTKYGEF QGKLYQDTIS GEHHLAIIKG 

       250        260        270        280        290        300 
EPDKEKEPVL VRVHSECLTG DVLGSMRCDC GDQLASAMQK IEEEGVGVVL YMRQEGRGIG 

       310        320        330        340        350        360 
LANKLKAYEL QDQGKDTVEA NELLGFPADL RDYGIGAQIL KDLGLTKILL MTNNPKKIIG 

       370        380        390        400        410 
LKGYGLEVEQ RLALEAEVTP HNKCYLQTKQ DKLGHILNVQ QQNDNKEENY YE

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References

[1]"Complete sequence of chromosome of Natranaerobius thermophilus JW/NM-WN-LF."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Lykidis A., Mesbah N.M., Wiegel J.
Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001034 Genomic DNA. Translation: ACB83723.1.
RefSeqYP_001916311.1. NC_010718.1.

3D structure databases

ProteinModelPortalB2A3U7.
ModBaseSearch...

Protein-protein interaction databases

STRING457570.Nther_0124.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACB83723; ACB83723; Nther_0124.
GeneID6317176.
KEGGnth:Nther_0124.
PATRIC22668846. VBINatThe92436_0141.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0108.
HOGENOMHOG000115440.
KOK14652.
OMALRCDCRM.

Enzyme and pathway databases

BioCycNTHE457570:GHRL-151-MONOMER.
UniPathwayUPA00275; UER00399.
UPA00275; UER00400.

Family and domain databases

Gene3D3.90.870.10. 1 hit.
HAMAPMF_00179. RibA.
MF_00180. RibB.
MF_01283. RibBA.
InterProIPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR000422. DHBP_synthase_RibB.
IPR000926. GTP_CycHdrlaseII_RibA.
IPR016299. Riboflavin_synth_RibBA.
[Graphical view]
PfamPF00926. DHBP_synthase. 1 hit.
PF00925. GTP_cyclohydro2. 1 hit.
[Graphical view]
PIRSFPIRSF001259. RibA. 1 hit.
SUPFAMSSF55821. DHBP_synth_RibB-like_a/b_dom. 1 hit.
TIGRFAMsTIGR00505. ribA. 1 hit.
TIGR00506. ribB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameB2A3U7_NATTJ
AccessionPrimary (citable) accession number: B2A3U7
Entry history
Integrated into UniProtKB/TrEMBL: May 20, 2008
Last sequence update: May 20, 2008
Last modified: May 1, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info