B2A3M9 (B2A3M9_NATTJ) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061
Short name=CMK HAMAP MF_00061
EC=2.7.1.148 HAMAP MF_00061

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase HAMAP MF_00061

Gene names

Name:	ispE HAMAP MF_00061
Ordered Locus Names:	Nther_0056

Organism

Natranaerobius thermophilus (strain ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF) [Complete proteome] [HAMAP]

Taxonomic identifier

457570 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Natranaerobiales › Natranaerobiaceae › Natranaerobius

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily. HAMAP MF_00061

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP MF_00061
Ligand	ATP-binding HAMAP MF_00061 Nucleotide-binding
Molecular function	Kinase HAMAP MF_00061 EMBL ACB83655.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	103 – 113	11	ATP By similarity HAMAP MF_00061
Sites
	Active site	13	1	By similarity HAMAP MF_00061
	Active site	145	1	By similarity HAMAP MF_00061

Sequences

	Sequence		Length	Mass (Da)
	B2A3M9 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: EE665C63E339494E	FASTA	308	34,367
	10 20 30 40 50 60 MSPDILRVES PAKINLYLDI LGKRQDGFHE VEMVMQSISL CDRLTFIRQS QGNNNNIELL 70 80 90 100 110 120 MKSSDCIDQL PVRGDNLIIK AARLLMNDFR LPPIKVILEK NIPTEAGLAG GSSNAAATLW 130 140 150 160 170 180 AINHMFQLGL TEQELSDIGA RLGSDIPFCL FGGTKLAKGR GEILHPLPSL PNCYFVLVKP 190 200 210 220 230 240 NFGVSTGKVY QELGFKTDTE YGENHQTKSR VNGIISGLEK GTLTGIVENM YNKMEEIVFK 250 260 270 280 290 300 WHRDMQIISQ QIEQLGALKV LMSGSGSTIF GVFDNYDTAK YAKKQLEREF KYVFLSIPRD MGVGKENN

References

[1]

"Complete sequence of chromosome of Natranaerobius thermophilus JW/NM-WN-LF." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Lykidis A., Mesbah N.M., Wiegel J. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001034 Genomic DNA. Translation: ACB83655.1.
RefSeq	YP_001916243.1. NC_010718.1.
3D structure databases
ProteinModelPortal	B2A3M9.
ModBase	Search...
Protein-protein interaction databases
STRING	B2A3M9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6316825.
GenomeReviews	Gene locus Nther_0056 in contig CP001034_GR.
KEGG	nth:Nther_0056.
PATRIC	22668698. VBINatThe92436_0073.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734593.
OMA	VSTAEVY.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B2A3M9_NATTJ

Accession

Primary (citable) accession number: B2A3M9

Entry history

Integrated into UniProtKB/TrEMBL:	May 20, 2008
Last sequence update:	May 20, 2008
Last modified:	December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)