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B2A2Z6 (DDL_NATTJ) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Nther_0011
OrganismNatranaerobius thermophilus (strain ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF) [Complete proteome] [HAMAP]
Taxonomic identifier457570 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaNatranaerobialesNatranaerobiaceaeNatranaerobius

Protein attributes

Sequence length365 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 365365D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000091196

Regions

Domain140 – 346207ATP-grasp
Nucleotide binding173 – 22856ATP By similarity

Sites

Metal binding2991Magnesium or manganese 1 By similarity
Metal binding3131Magnesium or manganese 1 By similarity
Metal binding3131Magnesium or manganese 2 By similarity
Metal binding3151Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
B2A2Z6 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: 9BB79AE18B548A86

FASTA36541,090
        10         20         30         40         50         60 
MKKLRVGVLF GGRSGEHEVS LKSANSILNA LDRELFEVIP IGIRKNGEWI SGEAPLEELE 

        70         80         90        100        110        120 
TGIKNQGNYA VSILPDPSKK VLWKLDPFEK ISEIDLIFPV LHGTFGEDGT VQGFLDLCGI 

       130        140        150        160        170        180 
PYVGSGVLGS SLAMDKVMMK KILRRHGLQV ANYYSFKRSE WEQDRDEIIF SIEKQLSYPI 

       190        200        210        220        230        240 
FVKPANLGSS VGISKVKNRE ELIQGIDLAV KYDMKCLAEE FIPGKEIELS ILGNDNPKTS 

       250        260        270        280        290        300 
VPGEIIPANE FYDYNAKYIN NKSKLVIPAP LSETLKTKIE DMGVEAFRVL DCYGLSRVDY 

       310        320        330        340        350        360 
FVLEDQEEVY INEINTMPGF TEISMYPKLW TESGLSYSQL LTDLIYLALA RQQEKDRNST 


DFSES 

« Hide

References

[1]"Complete sequence of chromosome of Natranaerobius thermophilus JW/NM-WN-LF."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Lykidis A., Mesbah N.M., Wiegel J.
Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001034 Genomic DNA. Translation: ACB83610.1.
RefSeqYP_001916198.1. NC_010718.1.

3D structure databases

ProteinModelPortalB2A2Z6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING457570.Nther_0011.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACB83610; ACB83610; Nther_0011.
GeneID6314408.
KEGGnth:Nther_0011.
PATRIC22668598. VBINatThe92436_0028.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAMDKIAMK.
OrthoDBEOG64BQ73.

Enzyme and pathway databases

BioCycNTHE457570:GHRL-16-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_NATTJ
AccessionPrimary (citable) accession number: B2A2Z6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 20, 2008
Last modified: February 19, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways