B2A2N1 (RNC_NATTJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease 3
EC=3.1.26.3

Alternative name(s):
Ribonuclease III
Short name=RNase III

Gene names

Name:	rnc
Ordered Locus Names:	Nther_1363

Organism

Natranaerobius thermophilus (strain ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF) [Complete proteome] [HAMAP]

Taxonomic identifier

457570 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Natranaerobiales › Natranaerobiaceae › Natranaerobius › Natranaerobius thermophilus

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon By similarity. HAMAP-Rule MF_00104
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00104
Cofactor	Mg2+ By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 RNase III domain.

Ontologies

Keywords
Biological process	mRNA processing rRNA processing tRNA processing
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding RNA-binding rRNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	mRNA processing Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC rRNA catabolic process Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: HAMAP tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease III activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 230	230	Ribonuclease 3 HAMAP-Rule MF_00104		PRO_1000094121
Regions
	Domain	8 – 135	128	RNase III
	Domain	161 – 230	70	DRBM
Sites
	Active site	52	1	Potential
	Active site	124	1	By similarity
	Metal binding	48	1	Magnesium By similarity
	Metal binding	121	1	Magnesium By similarity
	Metal binding	124	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2A2N1 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 666C7C1447C62F7A	FASTA	230	26,040
	10 20 30 40 50 60 MGKECRDIVE LKEKLGIYVD DKLLLQAVTH TSYAHEQKDV VDHNERLEFL GDAVLELAIS 70 80 90 100 110 120 ETLYKKYPEL PEGELTKLRA ELVCELSLVK IAEKLDLGKY LRLGKGEDST GGRDRRSTLA 130 140 150 160 170 180 DTVEALIGAV YLQTNYDQTK QLILDLFKDQ LSHIDNQRIG DYKTMIQELV QDRYGDPPKY 190 200 210 220 230 QIVKESGPDH DKSFVAEVQI NNEVVGRGSG KSKKEAEQNA AHFAFQKLSK

References

[1]

"Complete sequence of chromosome of Natranaerobius thermophilus JW/NM-WN-LF." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Lykidis A., Mesbah N.M., Wiegel J. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001034 Genomic DNA. Translation: ACB84946.1.
RefSeq	YP_001917534.1. NC_010718.1.
3D structure databases
ProteinModelPortal	B2A2N1.
ModBase	Search...
Protein-protein interaction databases
STRING	457570.Nther_1363.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACB84946; ACB84946; Nther_1363.
GeneID	6314537.
KEGG	nth:Nther_1363.
PATRIC	22671392. VBINatThe92436_1398.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0571.
HOGENOM	HOG000246808.
KO	K03685.
OMA	LTHKSCK.
Enzyme and pathway databases
BioCyc	NTHE457570:GHRL-1409-MONOMER.
Family and domain databases
Gene3D	1.10.1520.10. 1 hit. 3.30.160.20. 1 hit.
HAMAP	MF_00104. RNase_III.
InterPro	IPR001159. Ds-RNA-bd. IPR014720. dsRNA-bd-like_dom. IPR011907. RNase_III. IPR000999. RNase_III_dom. [Graphical view]
PANTHER	PTHR11207. PTHR11207. 1 hit.
Pfam	PF00035. dsrm. 1 hit. PF00636. Ribonuclease_3. 1 hit. [Graphical view]
SMART	SM00358. DSRM. 1 hit. SM00535. RIBOc. 1 hit. [Graphical view]
SUPFAM	SSF69065. RNase_III. 1 hit.
TIGRFAMs	TIGR02191. RNaseIII. 1 hit.
PROSITE	PS50137. DS_RBD. 1 hit. PS00517. RNASE_3_1. 1 hit. PS50142. RNASE_3_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNC_NATTJ

Accession

Primary (citable) accession number: B2A2N1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 20, 2008
Last modified:	May 1, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program