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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Natranaerobius thermophilus (strain ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei555Aminoacyl-adenylateUniRule annotation1
Binding sitei599ATPUniRule annotation1
Metal bindingi897ZincUniRule annotation1
Metal bindingi900ZincUniRule annotation1
Metal bindingi914ZincUniRule annotation1
Metal bindingi917ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Nther_1320
OrganismiNatranaerobius thermophilus (strain ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF)
Taxonomic identifieri457570 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaNatranaerobialesNatranaerobiaceaeNatranaerobius
Proteomesi
  • UP000001683 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001891821 – 926Isoleucine--tRNA ligaseAdd BLAST926

Proteomic databases

PRIDEiB2A2I8

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi457570.Nther_1320

Structurei

3D structure databases

ProteinModelPortaliB2A2I8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionAdd BLAST11
Motifi596 – 600"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.6202 hits
3.90.740.101 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

B2A2I8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYKDTLNLP KTDFPMKAKL PSREPEFLQE WESNNLYQRV QQKRSGKPKY
60 70 80 90 100
ILHDGPPYAN GNIHMGHALN KVLKDIVVKF KTMQGYDSPY VPGWDTHGLP
110 120 130 140 150
IEHQITKTEK VDRKSMSDVE FRKKCHDYAM KYVEIQKEEF KRLGVRGDWD
160 170 180 190 200
NPYLTLSPEF EAEQVKLFGE MAQKGYIYKG LKPVYWCTDC ETALAEAEVE
210 220 230 240 250
YHDKRSPSIY VGFHVKDSKG EFNEEGVEFI IWTTTPWTIP ANMAIALHPE
260 270 280 290 300
FQYSLIKSGE KHYIVATDLL ETVAEEAKLG EYQIIREYTG RELEGIVCQH
310 320 330 340 350
PLFDSRESLV ILGDHVTLEQ GTGCVHTAPG HGHEDYEVAQ KYDLEVLSPL
360 370 380 390 400
NDSGVFTEEA GQFQGLYYDK ANKEITQALD KRGALLSLSF ITHQYPCCWR
410 420 430 440 450
CKESVIFRAT EQWFASVDGF RQDALKAIED VDWIPAWGEE RIKAMVMNRG
460 470 480 490 500
DWCISRQRVW GVPLPIFYCQ ECGHELITEE SISAVAELFR QEGSDAWFEK
510 520 530 540 550
EAPEILPQGI QCSCGAKKFS KETDIMDVWF DSGSTHRGVC AQRQELAWPV
560 570 580 590 600
DLYLEGSDQY RGWFQSSLLT AVATKGESPY RECLTNGWVV DGEGKKMSKS
610 620 630 640 650
QGNVIAPQDI TNQYGADILR LWVASSEFKQ DVRVSQKILK QTAEAYRKIR
660 670 680 690 700
NTARFILGNL YDFTPEKDYV SFDQLEEIDS YILCRLQKVI DQATRAYDEF
710 720 730 740 750
EFHEFYHLIH NFCVVELSQF YLDVIKDRIY TMPTESRERR AAQTTMYYLL
760 770 780 790 800
DSLVKMLAPV LTFTSEEIWQ YLPGDREESI QLTDWPEVNE ELVDNELEQK
810 820 830 840 850
WANFLEFRKE VAKALENARK DKKIGSSLES KILIYADEDL FKKLQSFEDN
860 870 880 890 900
LEELFIVSQV ELKKAEQLTE TVKNQALSSE DVEQASIIIE AAQGEKCPRC
910 920
WNYHPEVTKA EELCPRCSHV LETSNK
Length:926
Mass (Da):106,964
Last modified:May 20, 2008 - v1
Checksum:i5D98C9332F25718F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001034 Genomic DNA Translation: ACB84903.1
RefSeqiWP_012447778.1, NC_010718.1

Genome annotation databases

EnsemblBacteriaiACB84903; ACB84903; Nther_1320
KEGGinth:Nther_1320

Similar proteinsi

Entry informationi

Entry nameiSYI_NATTJ
AccessioniPrimary (citable) accession number: B2A2I8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 20, 2008
Last modified: March 28, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome