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B2A2I8

- SYI_NATTJ

UniProt

B2A2I8 - SYI_NATTJ

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Protein

Isoleucine--tRNA ligase

Gene
ileS, Nther_1320
Organism
Natranaerobius thermophilus (strain ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei555 – 5551Aminoacyl-adenylate By similarity
Binding sitei599 – 5991ATP By similarity
Metal bindingi897 – 8971Zinc By similarity
Metal bindingi900 – 9001Zinc By similarity
Metal bindingi914 – 9141Zinc By similarity
Metal bindingi917 – 9171Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciNTHE457570:GHRL-1356-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Nther_1320
OrganismiNatranaerobius thermophilus (strain ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF)
Taxonomic identifieri457570 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaNatranaerobialesNatranaerobiaceaeNatranaerobius
ProteomesiUP000001683: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 926926Isoleucine--tRNA ligaseUniRule annotationPRO_1000189182Add
BLAST

Proteomic databases

PRIDEiB2A2I8.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi457570.Nther_1320.

Structurei

3D structure databases

ProteinModelPortaliB2A2I8.
SMRiB2A2I8. Positions 1-923.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotationAdd
BLAST
Motifi596 – 6005"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2A2I8-1 [UniParc]FASTAAdd to Basket

« Hide

MNYKDTLNLP KTDFPMKAKL PSREPEFLQE WESNNLYQRV QQKRSGKPKY    50
ILHDGPPYAN GNIHMGHALN KVLKDIVVKF KTMQGYDSPY VPGWDTHGLP 100
IEHQITKTEK VDRKSMSDVE FRKKCHDYAM KYVEIQKEEF KRLGVRGDWD 150
NPYLTLSPEF EAEQVKLFGE MAQKGYIYKG LKPVYWCTDC ETALAEAEVE 200
YHDKRSPSIY VGFHVKDSKG EFNEEGVEFI IWTTTPWTIP ANMAIALHPE 250
FQYSLIKSGE KHYIVATDLL ETVAEEAKLG EYQIIREYTG RELEGIVCQH 300
PLFDSRESLV ILGDHVTLEQ GTGCVHTAPG HGHEDYEVAQ KYDLEVLSPL 350
NDSGVFTEEA GQFQGLYYDK ANKEITQALD KRGALLSLSF ITHQYPCCWR 400
CKESVIFRAT EQWFASVDGF RQDALKAIED VDWIPAWGEE RIKAMVMNRG 450
DWCISRQRVW GVPLPIFYCQ ECGHELITEE SISAVAELFR QEGSDAWFEK 500
EAPEILPQGI QCSCGAKKFS KETDIMDVWF DSGSTHRGVC AQRQELAWPV 550
DLYLEGSDQY RGWFQSSLLT AVATKGESPY RECLTNGWVV DGEGKKMSKS 600
QGNVIAPQDI TNQYGADILR LWVASSEFKQ DVRVSQKILK QTAEAYRKIR 650
NTARFILGNL YDFTPEKDYV SFDQLEEIDS YILCRLQKVI DQATRAYDEF 700
EFHEFYHLIH NFCVVELSQF YLDVIKDRIY TMPTESRERR AAQTTMYYLL 750
DSLVKMLAPV LTFTSEEIWQ YLPGDREESI QLTDWPEVNE ELVDNELEQK 800
WANFLEFRKE VAKALENARK DKKIGSSLES KILIYADEDL FKKLQSFEDN 850
LEELFIVSQV ELKKAEQLTE TVKNQALSSE DVEQASIIIE AAQGEKCPRC 900
WNYHPEVTKA EELCPRCSHV LETSNK 926
Length:926
Mass (Da):106,964
Last modified:May 20, 2008 - v1
Checksum:i5D98C9332F25718F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001034 Genomic DNA. Translation: ACB84903.1.
RefSeqiWP_012447778.1. NC_010718.1.
YP_001917491.1. NC_010718.1.

Genome annotation databases

EnsemblBacteriaiACB84903; ACB84903; Nther_1320.
GeneIDi6315558.
KEGGinth:Nther_1320.
PATRICi22671306. VBINatThe92436_1355.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001034 Genomic DNA. Translation: ACB84903.1 .
RefSeqi WP_012447778.1. NC_010718.1.
YP_001917491.1. NC_010718.1.

3D structure databases

ProteinModelPortali B2A2I8.
SMRi B2A2I8. Positions 1-923.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 457570.Nther_1320.

Proteomic databases

PRIDEi B2A2I8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACB84903 ; ACB84903 ; Nther_1320 .
GeneIDi 6315558.
KEGGi nth:Nther_1320.
PATRICi 22671306. VBINatThe92436_1355.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci NTHE457570:GHRL-1356-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome of Natranaerobius thermophilus JW/NM-WN-LF."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.
    , Kyrpides N., Lykidis A., Mesbah N.M., Wiegel J.
    Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF.

Entry informationi

Entry nameiSYI_NATTJ
AccessioniPrimary (citable) accession number: B2A2I8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 20, 2008
Last modified: September 3, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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