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B2A2I8

- SYI_NATTJ

UniProt

B2A2I8 - SYI_NATTJ

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Natranaerobius thermophilus (strain ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 51 (01 Oct 2014)
      Sequence version 1 (20 May 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei555 – 5551Aminoacyl-adenylateUniRule annotation
    Binding sitei599 – 5991ATPUniRule annotation
    Metal bindingi897 – 8971ZincUniRule annotation
    Metal bindingi900 – 9001ZincUniRule annotation
    Metal bindingi914 – 9141ZincUniRule annotation
    Metal bindingi917 – 9171ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciNTHE457570:GHRL-1356-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Nther_1320
    OrganismiNatranaerobius thermophilus (strain ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF)
    Taxonomic identifieri457570 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaNatranaerobialesNatranaerobiaceaeNatranaerobius
    ProteomesiUP000001683: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 926926Isoleucine--tRNA ligasePRO_1000189182Add
    BLAST

    Proteomic databases

    PRIDEiB2A2I8.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi457570.Nther_1320.

    Structurei

    3D structure databases

    ProteinModelPortaliB2A2I8.
    SMRiB2A2I8. Positions 1-923.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi596 – 6005"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B2A2I8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNYKDTLNLP KTDFPMKAKL PSREPEFLQE WESNNLYQRV QQKRSGKPKY    50
    ILHDGPPYAN GNIHMGHALN KVLKDIVVKF KTMQGYDSPY VPGWDTHGLP 100
    IEHQITKTEK VDRKSMSDVE FRKKCHDYAM KYVEIQKEEF KRLGVRGDWD 150
    NPYLTLSPEF EAEQVKLFGE MAQKGYIYKG LKPVYWCTDC ETALAEAEVE 200
    YHDKRSPSIY VGFHVKDSKG EFNEEGVEFI IWTTTPWTIP ANMAIALHPE 250
    FQYSLIKSGE KHYIVATDLL ETVAEEAKLG EYQIIREYTG RELEGIVCQH 300
    PLFDSRESLV ILGDHVTLEQ GTGCVHTAPG HGHEDYEVAQ KYDLEVLSPL 350
    NDSGVFTEEA GQFQGLYYDK ANKEITQALD KRGALLSLSF ITHQYPCCWR 400
    CKESVIFRAT EQWFASVDGF RQDALKAIED VDWIPAWGEE RIKAMVMNRG 450
    DWCISRQRVW GVPLPIFYCQ ECGHELITEE SISAVAELFR QEGSDAWFEK 500
    EAPEILPQGI QCSCGAKKFS KETDIMDVWF DSGSTHRGVC AQRQELAWPV 550
    DLYLEGSDQY RGWFQSSLLT AVATKGESPY RECLTNGWVV DGEGKKMSKS 600
    QGNVIAPQDI TNQYGADILR LWVASSEFKQ DVRVSQKILK QTAEAYRKIR 650
    NTARFILGNL YDFTPEKDYV SFDQLEEIDS YILCRLQKVI DQATRAYDEF 700
    EFHEFYHLIH NFCVVELSQF YLDVIKDRIY TMPTESRERR AAQTTMYYLL 750
    DSLVKMLAPV LTFTSEEIWQ YLPGDREESI QLTDWPEVNE ELVDNELEQK 800
    WANFLEFRKE VAKALENARK DKKIGSSLES KILIYADEDL FKKLQSFEDN 850
    LEELFIVSQV ELKKAEQLTE TVKNQALSSE DVEQASIIIE AAQGEKCPRC 900
    WNYHPEVTKA EELCPRCSHV LETSNK 926
    Length:926
    Mass (Da):106,964
    Last modified:May 20, 2008 - v1
    Checksum:i5D98C9332F25718F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001034 Genomic DNA. Translation: ACB84903.1.
    RefSeqiWP_012447778.1. NC_010718.1.
    YP_001917491.1. NC_010718.1.

    Genome annotation databases

    EnsemblBacteriaiACB84903; ACB84903; Nther_1320.
    GeneIDi6315558.
    KEGGinth:Nther_1320.
    PATRICi22671306. VBINatThe92436_1355.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001034 Genomic DNA. Translation: ACB84903.1 .
    RefSeqi WP_012447778.1. NC_010718.1.
    YP_001917491.1. NC_010718.1.

    3D structure databases

    ProteinModelPortali B2A2I8.
    SMRi B2A2I8. Positions 1-923.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 457570.Nther_1320.

    Proteomic databases

    PRIDEi B2A2I8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACB84903 ; ACB84903 ; Nther_1320 .
    GeneIDi 6315558.
    KEGGi nth:Nther_1320.
    PATRICi 22671306. VBINatThe92436_1355.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci NTHE457570:GHRL-1356-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of chromosome of Natranaerobius thermophilus JW/NM-WN-LF."
      US DOE Joint Genome Institute
      Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.
      , Kyrpides N., Lykidis A., Mesbah N.M., Wiegel J.
      Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF.

    Entry informationi

    Entry nameiSYI_NATTJ
    AccessioniPrimary (citable) accession number: B2A2I8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: May 20, 2008
    Last modified: October 1, 2014
    This is version 51 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3