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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Natranaerobius thermophilus (strain ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei555 – 5551Aminoacyl-adenylateUniRule annotation
Binding sitei599 – 5991ATPUniRule annotation
Metal bindingi897 – 8971ZincUniRule annotation
Metal bindingi900 – 9001ZincUniRule annotation
Metal bindingi914 – 9141ZincUniRule annotation
Metal bindingi917 – 9171ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciNTHE457570:GHRL-1356-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Nther_1320
OrganismiNatranaerobius thermophilus (strain ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF)
Taxonomic identifieri457570 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaNatranaerobialesNatranaerobiaceaeNatranaerobius
ProteomesiUP000001683: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 926926Isoleucine--tRNA ligasePRO_1000189182Add
BLAST

Proteomic databases

PRIDEiB2A2I8.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi457570.Nther_1320.

Structurei

3D structure databases

ProteinModelPortaliB2A2I8.
SMRiB2A2I8. Positions 1-923.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi596 – 6005"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2A2I8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNYKDTLNLP KTDFPMKAKL PSREPEFLQE WESNNLYQRV QQKRSGKPKY
60 70 80 90 100
ILHDGPPYAN GNIHMGHALN KVLKDIVVKF KTMQGYDSPY VPGWDTHGLP
110 120 130 140 150
IEHQITKTEK VDRKSMSDVE FRKKCHDYAM KYVEIQKEEF KRLGVRGDWD
160 170 180 190 200
NPYLTLSPEF EAEQVKLFGE MAQKGYIYKG LKPVYWCTDC ETALAEAEVE
210 220 230 240 250
YHDKRSPSIY VGFHVKDSKG EFNEEGVEFI IWTTTPWTIP ANMAIALHPE
260 270 280 290 300
FQYSLIKSGE KHYIVATDLL ETVAEEAKLG EYQIIREYTG RELEGIVCQH
310 320 330 340 350
PLFDSRESLV ILGDHVTLEQ GTGCVHTAPG HGHEDYEVAQ KYDLEVLSPL
360 370 380 390 400
NDSGVFTEEA GQFQGLYYDK ANKEITQALD KRGALLSLSF ITHQYPCCWR
410 420 430 440 450
CKESVIFRAT EQWFASVDGF RQDALKAIED VDWIPAWGEE RIKAMVMNRG
460 470 480 490 500
DWCISRQRVW GVPLPIFYCQ ECGHELITEE SISAVAELFR QEGSDAWFEK
510 520 530 540 550
EAPEILPQGI QCSCGAKKFS KETDIMDVWF DSGSTHRGVC AQRQELAWPV
560 570 580 590 600
DLYLEGSDQY RGWFQSSLLT AVATKGESPY RECLTNGWVV DGEGKKMSKS
610 620 630 640 650
QGNVIAPQDI TNQYGADILR LWVASSEFKQ DVRVSQKILK QTAEAYRKIR
660 670 680 690 700
NTARFILGNL YDFTPEKDYV SFDQLEEIDS YILCRLQKVI DQATRAYDEF
710 720 730 740 750
EFHEFYHLIH NFCVVELSQF YLDVIKDRIY TMPTESRERR AAQTTMYYLL
760 770 780 790 800
DSLVKMLAPV LTFTSEEIWQ YLPGDREESI QLTDWPEVNE ELVDNELEQK
810 820 830 840 850
WANFLEFRKE VAKALENARK DKKIGSSLES KILIYADEDL FKKLQSFEDN
860 870 880 890 900
LEELFIVSQV ELKKAEQLTE TVKNQALSSE DVEQASIIIE AAQGEKCPRC
910 920
WNYHPEVTKA EELCPRCSHV LETSNK
Length:926
Mass (Da):106,964
Last modified:May 20, 2008 - v1
Checksum:i5D98C9332F25718F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001034 Genomic DNA. Translation: ACB84903.1.
RefSeqiWP_012447778.1. NC_010718.1.
YP_001917491.1. NC_010718.1.

Genome annotation databases

EnsemblBacteriaiACB84903; ACB84903; Nther_1320.
GeneIDi6315558.
KEGGinth:Nther_1320.
PATRICi22671306. VBINatThe92436_1355.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001034 Genomic DNA. Translation: ACB84903.1.
RefSeqiWP_012447778.1. NC_010718.1.
YP_001917491.1. NC_010718.1.

3D structure databases

ProteinModelPortaliB2A2I8.
SMRiB2A2I8. Positions 1-923.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi457570.Nther_1320.

Proteomic databases

PRIDEiB2A2I8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACB84903; ACB84903; Nther_1320.
GeneIDi6315558.
KEGGinth:Nther_1320.
PATRICi22671306. VBINatThe92436_1355.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciNTHE457570:GHRL-1356-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome of Natranaerobius thermophilus JW/NM-WN-LF."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.
    , Kyrpides N., Lykidis A., Mesbah N.M., Wiegel J.
    Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF.

Entry informationi

Entry nameiSYI_NATTJ
AccessioniPrimary (citable) accession number: B2A2I8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 20, 2008
Last modified: January 7, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.