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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Opitutus terrae (strain DSM 11246 / PB90-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei228 – 2281ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciOTER452637:GHBR-2699-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Oter_2646
OrganismiOpitutus terrae (strain DSM 11246 / PB90-1)
Taxonomic identifieri452637 [NCBI]
Taxonomic lineageiBacteriaVerrucomicrobiaOpitutaeOpitutalesOpitutaceaeOpitutus
ProteomesiUP000007013 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 460460Glutamate--tRNA ligasePRO_0000367724Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi452637.Oter_2646.

Structurei

3D structure databases

ProteinModelPortaliB1ZUV7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi10 – 2011"HIGH" regionAdd
BLAST
Motifi225 – 2295"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiCRERTEP.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 2 hits.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.

Sequencei

Sequence statusi: Complete.

B1ZUV7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHVRVRFAP SPTGFFHIGS ARTALFNWLY ARHTGGTFVL RIEDTDKERN
60 70 80 90 100
SEAFLNVIYD SLRWLGMEWD EGPGKGGEFG PYRQSERDAV YREYLAKLTA
110 120 130 140 150
AGRTYEKDGA IWFKLLGERY EVFDEHRKKT VTKVKTAPTV IEDRIRGRVE
160 170 180 190 200
RVEDEDFVIF RSDGNPVFHF VNVVDDIAMQ ITHVIRGEDH LSNTSKHVEL
210 220 230 240 250
FKAFGAPVPQ FAHIPLILKQ NGPGKMSKRD QGALIEEYQR RGYLSEALVN
260 270 280 290 300
FLSLLGWNPG DDREKMPIAE IIRLFDLPGV NQSNARFDDK KLAHMNMAYL
310 320 330 340 350
LELPADTFVQ KARAFFESLG DNATAKAALA AEPAFFREVM LLSQPKIKGF
360 370 380 390 400
EELPAYTAYF FTDEFATDAK VRDKVMGKGD PKARLRELIE ALPGFDFSND
410 420 430 440 450
AAVEEGIKVL ATKNALGFGD YQSVARLGVS GTNVGPSITG MFRVLGRERV
460
RARLERLLNA
Length:460
Mass (Da):51,927
Last modified:May 20, 2008 - v1
Checksum:iC1F34FE57EB80CBA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001032 Genomic DNA. Translation: ACB75927.1.
RefSeqiWP_012375462.1. NC_010571.1.
YP_001819527.1. NC_010571.1.

Genome annotation databases

EnsemblBacteriaiACB75927; ACB75927; Oter_2646.
KEGGiote:Oter_2646.
PATRICi22816961. VBIOpiTer17422_2753.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001032 Genomic DNA. Translation: ACB75927.1.
RefSeqiWP_012375462.1. NC_010571.1.
YP_001819527.1. NC_010571.1.

3D structure databases

ProteinModelPortaliB1ZUV7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi452637.Oter_2646.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACB75927; ACB75927; Oter_2646.
KEGGiote:Oter_2646.
PATRICi22816961. VBIOpiTer17422_2753.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiCRERTEP.
OrthoDBiEOG6DRPF7.

Enzyme and pathway databases

BioCyciOTER452637:GHBR-2699-MONOMER.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 2 hits.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 11246 / PB90-1.

Entry informationi

Entry nameiSYE_OPITP
AccessioniPrimary (citable) accession number: B1ZUV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 20, 2008
Last modified: May 27, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.