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B1ZUV7 (SYE_OPITP) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:Oter_2646
OrganismOpitutus terrae (strain DSM 11246 / PB90-1) [Complete proteome] [HAMAP]
Taxonomic identifier452637 [NCBI]
Taxonomic lineageBacteriaVerrucomicrobiaOpitutaeOpitutalesOpitutaceaeOpitutus

Protein attributes

Sequence length460 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 460460Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_0000367724

Regions

Motif10 – 2011"HIGH" region HAMAP-Rule MF_00022
Motif225 – 2295"KMSKS" region HAMAP-Rule MF_00022

Sites

Binding site2281ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B1ZUV7 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: C1F34FE57EB80CBA

FASTA46051,927
        10         20         30         40         50         60 
MAHVRVRFAP SPTGFFHIGS ARTALFNWLY ARHTGGTFVL RIEDTDKERN SEAFLNVIYD 

        70         80         90        100        110        120 
SLRWLGMEWD EGPGKGGEFG PYRQSERDAV YREYLAKLTA AGRTYEKDGA IWFKLLGERY 

       130        140        150        160        170        180 
EVFDEHRKKT VTKVKTAPTV IEDRIRGRVE RVEDEDFVIF RSDGNPVFHF VNVVDDIAMQ 

       190        200        210        220        230        240 
ITHVIRGEDH LSNTSKHVEL FKAFGAPVPQ FAHIPLILKQ NGPGKMSKRD QGALIEEYQR 

       250        260        270        280        290        300 
RGYLSEALVN FLSLLGWNPG DDREKMPIAE IIRLFDLPGV NQSNARFDDK KLAHMNMAYL 

       310        320        330        340        350        360 
LELPADTFVQ KARAFFESLG DNATAKAALA AEPAFFREVM LLSQPKIKGF EELPAYTAYF 

       370        380        390        400        410        420 
FTDEFATDAK VRDKVMGKGD PKARLRELIE ALPGFDFSND AAVEEGIKVL ATKNALGFGD 

       430        440        450        460 
YQSVARLGVS GTNVGPSITG MFRVLGRERV RARLERLLNA 

« Hide

References

[1]"Genome sequence of the verrucomicrobium Opitutus terrae PB90-1, an abundant inhabitant of rice paddy soil ecosystems."
van Passel M.W., Kant R., Palva A., Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Pitluck S., Goltsman E., Clum A., Sun H., Schmutz J., Larimer F.W., Land M.L., Hauser L., Kyrpides N., Mikhailova N., Richardson P.P. expand/collapse author list , Janssen P.H., de Vos W.M., Smidt H.
J. Bacteriol. 193:2367-2368(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 11246 / PB90-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001032 Genomic DNA. Translation: ACB75927.1.
RefSeqYP_001819527.1. NC_010571.1.

3D structure databases

ProteinModelPortalB1ZUV7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING452637.Oter_2646.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACB75927; ACB75927; Oter_2646.
GeneID6206933.
KEGGote:Oter_2646.
PATRIC22816961. VBIOpiTer17422_2753.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000252720.
KOK01885.
OMAKRDANIM.
OrthoDBEOG6DRPF7.

Enzyme and pathway databases

BioCycOTER452637:GHBR-2699-MONOMER.

Family and domain databases

Gene3D1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_B. Glu_tRNA_synth_B.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 2 hits.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. SSF48163. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYE_OPITP
AccessionPrimary (citable) accession number: B1ZUV7
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 20, 2008
Last modified: May 14, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries