B1ZKT9 (B1ZKT9_METPB) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Ordered Locus Names:

Mpop_3508

Organism

Methylobacterium populi (strain ATCC BAA-705 / NCIMB 13946 / BJ001) [Complete proteome] [HAMAP]

Taxonomic identifier

441620 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	909 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding RuleBase RU000518 SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase RuleBase RU000518 SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B1ZKT9 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 3261A71E78330647	FASTA	909	98,567
	10 20 30 40 50 60 MKVVVVESPA KAKTINKYLG RDYEVLASFG HIRDLPAKDG SVDPEADFAM VWELEDRGSK 70 80 90 100 110 120 RVSEIARAIK GAEKLILATD PDREGEAISW HVIEALSARK ALKGIPVERV TFNAITKASV 130 140 150 160 170 180 DAAMRKPREI DQALVDAYLA RRALDYLVGF NLSPVLWRKL PGARSAGRVQ SVALRLVVER 190 200 210 220 230 240 EMEIERFKPR EYWSIVATLA TEAGGVFEAR LVGADGKRIQ RLDVGNAEEA AAFKRDLELA 250 260 270 280 290 300 TFQVASVEAK PAKRHPQPPF TTSTLQQEAS RKLGMAPAQT MRVAQKLYEG VDVGGETVGI 310 320 330 340 350 360 ITYMRTDGVD MAPEAISDAR RVIGREFGDD YVPGAPRKYS VKAKNAQEAH EAVRPTDMGR 370 380 390 400 410 420 LPKQVARYLE PEQAKLYELI WTRTIASQME SAELERTTVE VTASVGPRRI DLRATGQVVK 430 440 450 460 470 480 FDGFLALYQE GKDDEEDEDS KRLPAMAAGD PLKRERIAST QHFTEPPPRY SEASLVKRME 490 500 510 520 530 540 ELGIGRPSTY AAVLQTLRDR EYVKIEKKRL QPEDKGRLVT GFLESFFKRY VEYDFTASLE 550 560 570 580 590 600 EQLDRVSNAE IDWRSVLRDF WRDFSAAIGG TKELRVAEVL EALNGLLGPH IFPEKADGGD 610 620 630 640 650 660 PRACPTCGSG QLSLKLGKFG AFIGCSNYPE CKYTRQLSAS GVEGEGDGSS AEGGQPGVRV 670 680 690 700 710 720 LGDDPATGLP VTLRDGRFGP FVQLGEASSE KGAEKPKRSS LPKGTSPGSV TLEIALRLLS 730 740 750 760 770 780 LPREVAKHPE TGEPILANLG RFGPYVQHGK TYANLGKDDD VLEIGANRAI DLIVAKEQGG 790 800 810 820 830 840 GRGRPSSDPG RALGPHPDGA ALVVKAGKYG PYVSDGSVNA TLPKTMAAES LTLEQAIELI 850 860 870 880 890 900 NAKRASGGGK KPVRKTATRA RTATKAADGA EAKKPAVRKT ASKTASKSTT KAAAKPAAAK KAAAKTKAS

References

[1]

"Complete sequence of chromosome of Methylobacterium populi BJ001." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Marx C., Richardson P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-705 / NCIMB 13946 / BJ001.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001029 Genomic DNA. Translation: ACB81658.1.
RefSeq	YP_001926193.1. NC_010725.1.
3D structure databases
ProteinModelPortal	B1ZKT9.
ModBase	Search...
Protein-protein interaction databases
STRING	B1ZKT9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6311456.
GenomeReviews	Gene locus Mpop_3508 in contig CP001029_GR.
KEGG	mpo:Mpop_3508.
PATRIC	22564322. VBIMetPop124700_3566.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG512935.
OMA	KKVMPRL.
ProtClustDB	PRK07561.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B1ZKT9_METPB

Accession

Primary (citable) accession number: B1ZKT9

Entry history

Integrated into UniProtKB/TrEMBL:	May 20, 2008
Last sequence update:	May 20, 2008
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)