B1ZIP6 (ISPE_METPB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Mpop_3302

Organism

Methylobacterium populi (strain ATCC BAA-705 / NCIMB 13946 / BJ001) [Complete proteome] [HAMAP]

Taxonomic identifier

441620 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 291	291	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_1000092097
Regions
	Nucleotide binding	97 – 107	11	ATP Potential
Sites
	Active site	11	1	By similarity
	Active site	139	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1ZIP6 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: F77DD61DF7A8EBBB	FASTA	291	29,268
	10 20 30 40 50 60 MSLLTTRAPA KINLTLHVLG RRPGDGYHAL ESLVAFAEVA DTLELLPGRD LALEVSGPTA 70 80 90 100 110 120 GPAGPLDDNL VLRAARHLAA GVDGLRLGAF RLHKQLPVAA GIGGGSSDAA AALRLLAELN 130 140 150 160 170 180 ALALDHPAVI AAARATGADV PVCLDPRARM MRGAGEEIGP VLELAPLPAV LVNPGVPVST 190 200 210 220 230 240 APVFKALGLA VGQRLDGAEH PGVAAGLDAD AVLGVIAPAR NDLEAPALTV APVIGEALAL 250 260 270 280 290 LRAQGGCRLA RMSGSGATVF AIFSDTAAAQ AAAGAVRVAQ PGWWVEPTRL A

References

[1]

"Complete sequence of chromosome of Methylobacterium populi BJ001." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Marx C., Richardson P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-705 / NCIMB 13946 / BJ001.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001029 Genomic DNA. Translation: ACB81453.1.
RefSeq	YP_001925988.1. NC_010725.1.
3D structure databases
ProteinModelPortal	B1ZIP6.
ModBase	Search...
Protein-protein interaction databases
STRING	B1ZIP6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6311161.
GenomeReviews	Gene locus Mpop_3302 in contig CP001029_GR.
KEGG	mpo:Mpop_3302.
PATRIC	22563904. VBIMetPop124700_3358.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734593.
OMA	VSTAEVY.
ProtClustDB	PRK14608.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_METPB

Accession

Primary (citable) accession number: B1ZIP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program