B1ZIK6 (GATB_METPB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
Short name=Asp/Glu-ADT subunit B
EC=6.3.5.-

Gene names

Name:	gatB
Ordered Locus Names:	Mpop_3262

Organism

Methylobacterium populi (strain ATCC BAA-705 / NCIMB 13946 / BJ001) [Complete proteome] [HAMAP]

Taxonomic identifier

441620 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium › Methylobacterium populi

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) By similarity. HAMAP-Rule MF_00121
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP-Rule MF_00121 ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate. HAMAP-Rule MF_00121
Subunit structure	Heterotrimer of A, B and C subunits By similarity.
Sequence similarities	Belongs to the GatB/GatE family. GatB subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 490	490	Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B HAMAP-Rule MF_00121		PRO_1000095223

Sequences

	Sequence		Length	Mass (Da)
	B1ZIK6 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 7BC1A80829A6DF9D	FASTA	490	53,365
	10 20 30 40 50 60 MTERVDPKKL IKGGLHDWEV VIGMEIHAQV TSRSKLFSGA STAFGGEPND HVSLVDAAMP 70 80 90 100 110 120 GMLPVINEEC IAQAVRTGLG LKAQINLRSV FDRKNYFYPD LPQGYQISQY KDPIVGEGEV 130 140 150 160 170 180 LVDLPDGESI TVGIERLHLE QDAGKSLHDQ DPTKSFVDLN RSGVALMEIV SRPDLRSSEE 190 200 210 220 230 240 AKAYVTKLRT ILRYLGTCDG DMEKGSLRAD VNVSVRRPGE PLGTRCEIKN VNSIRFIGQA 250 260 270 280 290 300 IETEARRQIA ILEDGGKIDQ ETRLFDPGKG ETRSMRSKEE AHDYRYFPDP DLLPLEFDQA 310 320 330 340 350 360 YVDGLASGLP ELPDAKKARF IKDFGLSAYD AGVLVAERAS ADYFEAVARG RDGKAAANWV 370 380 390 400 410 420 INELFGRLNK EGRSIEDTPV SAEQLGTIVD LIGEGVISGK IAKDLFEIVW SEGGDPRAIV 430 440 450 460 470 480 ESRGMKQVTD TGAIEAAVDA IIAANPDKVE QAKAKPTLLG WFVGQTMKAT GGKANPAAVN 490 ALLKDKLGIE

References

[1]

"Complete sequence of chromosome of Methylobacterium populi BJ001." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Marx C., Richardson P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-705 / NCIMB 13946 / BJ001.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001029 Genomic DNA. Translation: ACB81413.1.
RefSeq	YP_001925948.1. NC_010725.1.
3D structure databases
ProteinModelPortal	B1ZIK6.
ModBase	Search...
Protein-protein interaction databases
STRING	441620.Mpop_3262.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACB81413; ACB81413; Mpop_3262.
GeneID	6311072.
KEGG	mpo:Mpop_3262.
PATRIC	22563820. VBIMetPop124700_3317.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0064.
HOGENOM	HOG000223743.
KO	K02434.
OMA	KNYFYAD.
ProtClustDB	CLSK2331496.
Enzyme and pathway databases
BioCyc	MPOP441620:GHMI-3343-MONOMER.
Family and domain databases
HAMAP	MF_00121. GatB.
InterPro	IPR004413. Apn/Gln-ADT_bsu. IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E. IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat. IPR018027. Asn/Gln_amidotransferase. IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel. IPR017958. Gln-tRNA_amidoTrfase_suB_CS. [Graphical view]
PANTHER	PTHR11659. PTHR11659. 1 hit.
Pfam	PF02934. GatB_N. 1 hit. PF02637. GatB_Yqey. 1 hit. [Graphical view]
SMART	SM00845. GatB_Yqey. 1 hit. [Graphical view]
SUPFAM	SSF89095. GatB_Yqey. 1 hit.
TIGRFAMs	TIGR00133. gatB. 1 hit.
PROSITE	PS01234. GATB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GATB_METPB

Accession

Primary (citable) accession number: B1ZIK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 20, 2008
Last modified:	May 1, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program