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B1ZGB0

- B1ZGB0_METPB

UniProt

B1ZGB0 - B1ZGB0_METPB

Protein

Phenylalanine--tRNA ligase beta subunit

Gene

pheT

Organism
Methylobacterium populi (strain ATCC BAA-705 / NCIMB 13946 / BJ001)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (20 May 2008)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).UniRule annotationSAAS annotation

    Cofactori

    Binds 2 magnesium ions per tetramer.UniRule annotationSAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi456 – 4561MagnesiumUniRule annotation
    Metal bindingi462 – 4621Magnesium; via carbonyl oxygenUniRule annotation
    Metal bindingi465 – 4651MagnesiumUniRule annotation
    Metal bindingi466 – 4661MagnesiumUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. phenylalanine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. tRNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. phenylalanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
    2. tRNA processing Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotationImported, Ligase

    Keywords - Biological processi

    Protein biosynthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, RNA-binding, tRNA-bindingUniRule annotationSAAS annotation

    Enzyme and pathway databases

    BioCyciMPOP441620:GHMI-1646-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phenylalanine--tRNA ligase beta subunitUniRule annotation (EC:6.1.1.20UniRule annotation)
    Alternative name(s):
    Phenylalanyl-tRNA synthetase beta subunitUniRule annotation
    Gene namesi
    Name:pheTUniRule annotation
    Ordered Locus Names:Mpop_1604Imported
    OrganismiMethylobacterium populi (strain ATCC BAA-705 / NCIMB 13946 / BJ001)Imported
    Taxonomic identifieri441620 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
    ProteomesiUP000007136: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    Interactioni

    Subunit structurei

    Tetramer of two alpha and two beta subunits.UniRule annotationSAAS annotation

    Protein-protein interaction databases

    STRINGi441620.Mpop_1604.

    Structurei

    3D structure databases

    ProteinModelPortaliB1ZGB0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini39 – 149111tRNA-bindingUniRule annotationAdd
    BLAST
    Domaini403 – 47876B5UniRule annotationAdd
    BLAST
    Domaini713 – 80694FDX-ACBUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily.UniRule annotation
    Contains 1 B5 domain.UniRule annotation
    Contains 1 FDX-ACB domain.UniRule annotation
    Contains 1 tRNA-binding domain.UniRule annotation
    Contains B5 domain.SAAS annotation
    Contains Bdomain.SAAS annotation
    Contains FDX-ACB domain.SAAS annotation
    Contains tRNA-binding domain.SAAS annotation

    Phylogenomic databases

    eggNOGiCOG0072.
    HOGENOMiHOG000292085.
    KOiK01890.
    OMAiNYVMIEL.
    OrthoDBiEOG6CCH1J.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    3.30.56.20. 1 hit.
    3.30.70.380. 1 hit.
    3.50.40.10. 1 hit.
    HAMAPiMF_00283. Phe_tRNA_synth_beta1.
    InterProiIPR005146. B3/B4_tRNA-bd.
    IPR009061. DNA-bd_dom_put.
    IPR012340. NA-bd_OB-fold.
    IPR004532. Phe-tRNA-ligase_IIc_bsu.
    IPR020825. Phe-tRNA_synthase_B3/B4.
    IPR005121. PheS_beta_Fdx_antiC-bd.
    IPR002547. tRNA-bd_dom.
    IPR005147. tRNA_synthase_B5-dom.
    [Graphical view]
    PfamiPF03483. B3_4. 1 hit.
    PF03484. B5. 1 hit.
    PF03147. FDX-ACB. 1 hit.
    PF01588. tRNA_bind. 1 hit.
    [Graphical view]
    SMARTiSM00873. B3_4. 1 hit.
    SM00874. B5. 1 hit.
    SM00896. FDX-ACB. 1 hit.
    [Graphical view]
    SUPFAMiSSF46955. SSF46955. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54991. SSF54991. 1 hit.
    SSF56037. SSF56037. 1 hit.
    TIGRFAMsiTIGR00472. pheT_bact. 1 hit.
    PROSITEiPS51483. B5. 1 hit.
    PS51447. FDX_ACB. 1 hit.
    PS50886. TRBD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B1ZGB0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKFTLSWLKD HLDTEASLDA ISETLTRIGL EVEGIEDKAA ALRPYVIARV    50
    ISAEQHPNAD RLRVCQVDPG DGQPLQVVCG APNARTGMLS VFAPPGTYVP 100
    GKAITLSVGT IRGVESRGML CSGAELGLSE DHDGILDLPA DAPVGTPYAV 150
    WAGLDDPVIE INLTPNRSDC ASIHGIARDL AATGIGTLKR EPMPPVRGEG 200
    ACPTPVTLQF DAHDKGLCPL FALRLVRGVK NGPSPEWMQK RLRAIGLRPI 250
    NALVDITNYL TFDRGRPLHV FDAKKVAGGL TVRRAEDGES LVALDGKTYR 300
    LDSDTVVIAD DKGVESIGGI MGGEASGCDE TTTDVLIESA LWDPRNIART 350
    GRRLGIITDA RYRFERGVDP AFALPGLDLA TRLVIDLCGG SPSQATIAGE 400
    VPELSHVIDF PWTEVRRLAG IELSRAEMKV TLESLGFHIS GSGDRVKVLP 450
    PSWRPDVEGK ADLVEEIVRI AGLDRIEPKP LPRIETVAVE PMLTVLQRRG 500
    RLAKRALASR GMLEAVTYSF IPHADARLFG GGKPDLALAN PIAADLSDMR 550
    PSLVPGLLRA AQANADRGFP DTALFEVGQC FASDEPEGQS LRATGLRRGT 600
    ARHSGAGRHW SGAAEVVDAF EAKADALALL AALGVPTGGL QIVAGGPDWL 650
    HPGRSGTLQF GPKNVVGHFG ELHPRLLKAM DLKGTLVAFE ITLDALPLPR 700
    HRPTKAKPAL ALSDLQAISR DFAFVVPRDV PAADILKAAQ GAERKMITGI 750
    EVFDLYEGTG IPEGAKSVAV AVRLQPSERT LTDAEIEAVS AKIVAEVGKK 800
    TGATLRS 807
    Length:807
    Mass (Da):85,916
    Last modified:May 20, 2008 - v1
    Checksum:i418AC8A705EB4640
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001029 Genomic DNA. Translation: ACB79768.1.
    RefSeqiYP_001924303.1. NC_010725.1.

    Genome annotation databases

    EnsemblBacteriaiACB79768; ACB79768; Mpop_1604.
    GeneIDi6312478.
    KEGGimpo:Mpop_1604.
    PATRICi22560441. VBIMetPop124700_1639.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001029 Genomic DNA. Translation: ACB79768.1 .
    RefSeqi YP_001924303.1. NC_010725.1.

    3D structure databases

    ProteinModelPortali B1ZGB0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 441620.Mpop_1604.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACB79768 ; ACB79768 ; Mpop_1604 .
    GeneIDi 6312478.
    KEGGi mpo:Mpop_1604.
    PATRICi 22560441. VBIMetPop124700_1639.

    Phylogenomic databases

    eggNOGi COG0072.
    HOGENOMi HOG000292085.
    KOi K01890.
    OMAi NYVMIEL.
    OrthoDBi EOG6CCH1J.

    Enzyme and pathway databases

    BioCyci MPOP441620:GHMI-1646-MONOMER.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    3.30.56.20. 1 hit.
    3.30.70.380. 1 hit.
    3.50.40.10. 1 hit.
    HAMAPi MF_00283. Phe_tRNA_synth_beta1.
    InterProi IPR005146. B3/B4_tRNA-bd.
    IPR009061. DNA-bd_dom_put.
    IPR012340. NA-bd_OB-fold.
    IPR004532. Phe-tRNA-ligase_IIc_bsu.
    IPR020825. Phe-tRNA_synthase_B3/B4.
    IPR005121. PheS_beta_Fdx_antiC-bd.
    IPR002547. tRNA-bd_dom.
    IPR005147. tRNA_synthase_B5-dom.
    [Graphical view ]
    Pfami PF03483. B3_4. 1 hit.
    PF03484. B5. 1 hit.
    PF03147. FDX-ACB. 1 hit.
    PF01588. tRNA_bind. 1 hit.
    [Graphical view ]
    SMARTi SM00873. B3_4. 1 hit.
    SM00874. B5. 1 hit.
    SM00896. FDX-ACB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46955. SSF46955. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54991. SSF54991. 1 hit.
    SSF56037. SSF56037. 1 hit.
    TIGRFAMsi TIGR00472. pheT_bact. 1 hit.
    PROSITEi PS51483. B5. 1 hit.
    PS51447. FDX_ACB. 1 hit.
    PS50886. TRBD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of chromosome of Methylobacterium populi BJ001."
      US DOE Joint Genome Institute
      Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.
      , Kyrpides N., Mikhailova N., Marx C., Richardson P.
      Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-705 / NCIMB 13946 / BJ001Imported.

    Entry informationi

    Entry nameiB1ZGB0_METPB
    AccessioniPrimary (citable) accession number: B1ZGB0
    Entry historyi
    Integrated into UniProtKB/TrEMBL: May 20, 2008
    Last sequence update: May 20, 2008
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3