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Protein

Phenylalanine--tRNA ligase beta subunit

Gene

pheT

Organism
Methylobacterium populi (strain ATCC BAA-705 / NCIMB 13946 / BJ001)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).UniRule annotationSAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationSAAS annotationNote: Binds 2 magnesium ions per tetramer.UniRule annotationSAAS annotation
  • Mg2+Note: Binds 2 magnesium ions per tetramer.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi456 – 4561MagnesiumUniRule annotation
Metal bindingi462 – 4621Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi465 – 4651MagnesiumUniRule annotation
Metal bindingi466 – 4661MagnesiumUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. phenylalanine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. phenylalanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
  2. tRNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotation, Ligase

Keywords - Biological processi

Protein biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, RNA-binding, tRNA-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciMPOP441620:GHMI-1646-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine--tRNA ligase beta subunitUniRule annotationSAAS annotation (EC:6.1.1.20UniRule annotationSAAS annotation)
Alternative name(s):
Phenylalanyl-tRNA synthetase beta subunitUniRule annotation
Gene namesi
Name:pheTUniRule annotation
Ordered Locus Names:Mpop_1604Imported
OrganismiMethylobacterium populi (strain ATCC BAA-705 / NCIMB 13946 / BJ001)Imported
Taxonomic identifieri441620 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
ProteomesiUP000007136 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi441620.Mpop_1604.

Structurei

3D structure databases

ProteinModelPortaliB1ZGB0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 149111tRNA-bindingUniRule annotationAdd
BLAST
Domaini403 – 47876B5UniRule annotationAdd
BLAST
Domaini713 – 80694FDX-ACBUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily.UniRule annotation
Contains 1 B5 domain.UniRule annotation
Contains 1 FDX-ACB domain.UniRule annotation
Contains 1 tRNA-binding domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0072.
HOGENOMiHOG000292085.
KOiK01890.
OMAiRQYGLHT.
OrthoDBiEOG6CCH1J.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.56.20. 1 hit.
3.30.70.380. 1 hit.
3.50.40.10. 1 hit.
HAMAPiMF_00283. Phe_tRNA_synth_beta1.
InterProiIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR012340. NA-bd_OB-fold.
IPR004532. Phe-tRNA-ligase_IIc_bsu.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR005121. PheS_beta_Fdx_antiC-bd.
IPR002547. tRNA-bd_dom.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamiPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
PF03147. FDX-ACB. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
SMARTiSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
SM00896. FDX-ACB. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54991. SSF54991. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR00472. pheT_bact. 1 hit.
PROSITEiPS51483. B5. 1 hit.
PS51447. FDX_ACB. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1ZGB0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFTLSWLKD HLDTEASLDA ISETLTRIGL EVEGIEDKAA ALRPYVIARV
60 70 80 90 100
ISAEQHPNAD RLRVCQVDPG DGQPLQVVCG APNARTGMLS VFAPPGTYVP
110 120 130 140 150
GKAITLSVGT IRGVESRGML CSGAELGLSE DHDGILDLPA DAPVGTPYAV
160 170 180 190 200
WAGLDDPVIE INLTPNRSDC ASIHGIARDL AATGIGTLKR EPMPPVRGEG
210 220 230 240 250
ACPTPVTLQF DAHDKGLCPL FALRLVRGVK NGPSPEWMQK RLRAIGLRPI
260 270 280 290 300
NALVDITNYL TFDRGRPLHV FDAKKVAGGL TVRRAEDGES LVALDGKTYR
310 320 330 340 350
LDSDTVVIAD DKGVESIGGI MGGEASGCDE TTTDVLIESA LWDPRNIART
360 370 380 390 400
GRRLGIITDA RYRFERGVDP AFALPGLDLA TRLVIDLCGG SPSQATIAGE
410 420 430 440 450
VPELSHVIDF PWTEVRRLAG IELSRAEMKV TLESLGFHIS GSGDRVKVLP
460 470 480 490 500
PSWRPDVEGK ADLVEEIVRI AGLDRIEPKP LPRIETVAVE PMLTVLQRRG
510 520 530 540 550
RLAKRALASR GMLEAVTYSF IPHADARLFG GGKPDLALAN PIAADLSDMR
560 570 580 590 600
PSLVPGLLRA AQANADRGFP DTALFEVGQC FASDEPEGQS LRATGLRRGT
610 620 630 640 650
ARHSGAGRHW SGAAEVVDAF EAKADALALL AALGVPTGGL QIVAGGPDWL
660 670 680 690 700
HPGRSGTLQF GPKNVVGHFG ELHPRLLKAM DLKGTLVAFE ITLDALPLPR
710 720 730 740 750
HRPTKAKPAL ALSDLQAISR DFAFVVPRDV PAADILKAAQ GAERKMITGI
760 770 780 790 800
EVFDLYEGTG IPEGAKSVAV AVRLQPSERT LTDAEIEAVS AKIVAEVGKK

TGATLRS
Length:807
Mass (Da):85,916
Last modified:May 20, 2008 - v1
Checksum:i418AC8A705EB4640
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001029 Genomic DNA. Translation: ACB79768.1.
RefSeqiYP_001924303.1. NC_010725.1.

Genome annotation databases

EnsemblBacteriaiACB79768; ACB79768; Mpop_1604.
KEGGimpo:Mpop_1604.
PATRICi22560441. VBIMetPop124700_1639.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001029 Genomic DNA. Translation: ACB79768.1.
RefSeqiYP_001924303.1. NC_010725.1.

3D structure databases

ProteinModelPortaliB1ZGB0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi441620.Mpop_1604.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACB79768; ACB79768; Mpop_1604.
KEGGimpo:Mpop_1604.
PATRICi22560441. VBIMetPop124700_1639.

Phylogenomic databases

eggNOGiCOG0072.
HOGENOMiHOG000292085.
KOiK01890.
OMAiRQYGLHT.
OrthoDBiEOG6CCH1J.

Enzyme and pathway databases

BioCyciMPOP441620:GHMI-1646-MONOMER.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.56.20. 1 hit.
3.30.70.380. 1 hit.
3.50.40.10. 1 hit.
HAMAPiMF_00283. Phe_tRNA_synth_beta1.
InterProiIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR012340. NA-bd_OB-fold.
IPR004532. Phe-tRNA-ligase_IIc_bsu.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR005121. PheS_beta_Fdx_antiC-bd.
IPR002547. tRNA-bd_dom.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamiPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
PF03147. FDX-ACB. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
SMARTiSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
SM00896. FDX-ACB. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54991. SSF54991. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR00472. pheT_bact. 1 hit.
PROSITEiPS51483. B5. 1 hit.
PS51447. FDX_ACB. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome of Methylobacterium populi BJ001."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.
    , Kyrpides N., Mikhailova N., Marx C., Richardson P.
    Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-705 / NCIMB 13946 / BJ001Imported.

Entry informationi

Entry nameiB1ZGB0_METPB
AccessioniPrimary (citable) accession number: B1ZGB0
Entry historyi
Integrated into UniProtKB/TrEMBL: May 20, 2008
Last sequence update: May 20, 2008
Last modified: April 1, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.