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B1ZFX7

- BIOB_METPB

UniProt

B1ZFX7 - BIOB_METPB

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Protein
Biotin synthase
Gene
bioB, Mpop_4487
Organism
Methylobacterium populi (strain ATCC BAA-705 / NCIMB 13946 / BJ001)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi71 – 711Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi75 – 751Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi78 – 781Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi116 – 1161Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi148 – 1481Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi208 – 2081Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi280 – 2801Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMPOP441620:GHMI-4551-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Mpop_4487
OrganismiMethylobacterium populi (strain ATCC BAA-705 / NCIMB 13946 / BJ001)
Taxonomic identifieri441620 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
ProteomesiUP000007136: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 341341Biotin synthaseUniRule annotation
PRO_0000381468Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi441620.Mpop_4487.

Structurei

3D structure databases

ProteinModelPortaliB1ZFX7.
SMRiB1ZFX7. Positions 22-331.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiDETQALC.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B1ZFX7-1 [UniParc]FASTAAdd to Basket

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MSDTVVSLAS SQAPDAVRQD IRHDWTLAEI QAIHDMPLLD LVHRAGTVHR    50
AHNDPADIQR AALLSIKTGG CPEDCAYCPQ SAHHKGANLP RERLMPVDAV 100
LKEAAAAKAN GAHRFCMGAA WRKPKDGPEF DAVLEMVRGV RGLGMEACVT 150
LGMLTQSQAQ RLAEAGLTSY NHNLDTGPEF YGDIISTRTY DDRLQTLEHV 200
RQAGIGVCCG GIVGMGERVR DRAEMLLVLA NHAPHPESVP INALVAVEGT 250
PLEDRPPIDP LDLVRMCATA RIVMPKARVR LSAGRKSLTR EAQILCFLAG 300
ANSIFYGERL LTTANNEADA DAELLRDIGV PVPEVTLAAA E 341
Length:341
Mass (Da):36,807
Last modified:May 20, 2008 - v1
Checksum:i5670FDE8E20DCE55
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001029 Genomic DNA. Translation: ACB82586.1.
RefSeqiYP_001927121.1. NC_010725.1.

Genome annotation databases

EnsemblBacteriaiACB82586; ACB82586; Mpop_4487.
GeneIDi6309280.
KEGGimpo:Mpop_4487.
PATRICi22566218. VBIMetPop124700_4505.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001029 Genomic DNA. Translation: ACB82586.1 .
RefSeqi YP_001927121.1. NC_010725.1.

3D structure databases

ProteinModelPortali B1ZFX7.
SMRi B1ZFX7. Positions 22-331.
ModBasei Search...

Protein-protein interaction databases

STRINGi 441620.Mpop_4487.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACB82586 ; ACB82586 ; Mpop_4487 .
GeneIDi 6309280.
KEGGi mpo:Mpop_4487.
PATRICi 22566218. VBIMetPop124700_4505.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi DETQALC.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci MPOP441620:GHMI-4551-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome of Methylobacterium populi BJ001."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.
    , Kyrpides N., Mikhailova N., Marx C., Richardson P.
    Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-705 / NCIMB 13946 / BJ001.

Entry informationi

Entry nameiBIOB_METPB
AccessioniPrimary (citable) accession number: B1ZFX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 20, 2008
Last modified: May 14, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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