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B1ZFX7 (BIOB_METPB) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Mpop_4487
OrganismMethylobacterium populi (strain ATCC BAA-705 / NCIMB 13946 / BJ001) [Complete proteome] [HAMAP]
Taxonomic identifier441620 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium

Protein attributes

Sequence length341 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 341341Biotin synthase HAMAP-Rule MF_01694
PRO_0000381468

Sites

Metal binding711Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding751Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding781Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1161Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1481Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2081Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2801Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B1ZFX7 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: 5670FDE8E20DCE55

FASTA34136,807
        10         20         30         40         50         60 
MSDTVVSLAS SQAPDAVRQD IRHDWTLAEI QAIHDMPLLD LVHRAGTVHR AHNDPADIQR 

        70         80         90        100        110        120 
AALLSIKTGG CPEDCAYCPQ SAHHKGANLP RERLMPVDAV LKEAAAAKAN GAHRFCMGAA 

       130        140        150        160        170        180 
WRKPKDGPEF DAVLEMVRGV RGLGMEACVT LGMLTQSQAQ RLAEAGLTSY NHNLDTGPEF 

       190        200        210        220        230        240 
YGDIISTRTY DDRLQTLEHV RQAGIGVCCG GIVGMGERVR DRAEMLLVLA NHAPHPESVP 

       250        260        270        280        290        300 
INALVAVEGT PLEDRPPIDP LDLVRMCATA RIVMPKARVR LSAGRKSLTR EAQILCFLAG 

       310        320        330        340 
ANSIFYGERL LTTANNEADA DAELLRDIGV PVPEVTLAAA E 

« Hide

References

[1]"Complete sequence of chromosome of Methylobacterium populi BJ001."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Mikhailova N., Marx C., Richardson P.
Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-705 / NCIMB 13946 / BJ001.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001029 Genomic DNA. Translation: ACB82586.1.
RefSeqYP_001927121.1. NC_010725.1.

3D structure databases

ProteinModelPortalB1ZFX7.
SMRB1ZFX7. Positions 22-331.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING441620.Mpop_4487.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACB82586; ACB82586; Mpop_4487.
GeneID6309280.
KEGGmpo:Mpop_4487.
PATRIC22566218. VBIMetPop124700_4505.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMAADRFCMG.
OrthoDBEOG622PMP.
ProtClustDBCLSK2331944.

Enzyme and pathway databases

BioCycMPOP441620:GHMI-4551-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_METPB
AccessionPrimary (citable) accession number: B1ZFX7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 20, 2008
Last modified: February 19, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways