B1ZFX7 (BIOB_METPB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Biotin synthase
EC=2.8.1.6

Gene names

Name:	bioB
Ordered Locus Names:	Mpop_4487

Organism

Methylobacterium populi (strain ATCC BAA-705 / NCIMB 13946 / BJ001) [Complete proteome] [HAMAP]

Taxonomic identifier

441620 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium › Methylobacterium populi

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694
Catalytic activity	Dethiobiotin + sulfur + 2 S-adenosyl-L-methionine = biotin + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity. Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the radical SAM superfamily. Biotin synthase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW biotin synthase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Biotin synthase HAMAP-Rule MF_01694		PRO_0000381468
Sites
	Metal binding	71	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	75	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	78	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	116	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	148	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	208	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	280	1	Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1ZFX7 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 5670FDE8E20DCE55	FASTA	341	36,807
	10 20 30 40 50 60 MSDTVVSLAS SQAPDAVRQD IRHDWTLAEI QAIHDMPLLD LVHRAGTVHR AHNDPADIQR 70 80 90 100 110 120 AALLSIKTGG CPEDCAYCPQ SAHHKGANLP RERLMPVDAV LKEAAAAKAN GAHRFCMGAA 130 140 150 160 170 180 WRKPKDGPEF DAVLEMVRGV RGLGMEACVT LGMLTQSQAQ RLAEAGLTSY NHNLDTGPEF 190 200 210 220 230 240 YGDIISTRTY DDRLQTLEHV RQAGIGVCCG GIVGMGERVR DRAEMLLVLA NHAPHPESVP 250 260 270 280 290 300 INALVAVEGT PLEDRPPIDP LDLVRMCATA RIVMPKARVR LSAGRKSLTR EAQILCFLAG 310 320 330 340 ANSIFYGERL LTTANNEADA DAELLRDIGV PVPEVTLAAA E

References

[1]

"Complete sequence of chromosome of Methylobacterium populi BJ001." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Marx C., Richardson P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-705 / NCIMB 13946 / BJ001.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001029 Genomic DNA. Translation: ACB82586.1.
RefSeq	YP_001927121.1. NC_010725.1.
3D structure databases
ProteinModelPortal	B1ZFX7.
SMR	B1ZFX7. Positions 22-331.
ModBase	Search...
Protein-protein interaction databases
STRING	441620.Mpop_4487.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACB82586; ACB82586; Mpop_4487.
GeneID	6309280.
KEGG	mpo:Mpop_4487.
PATRIC	22566218. VBIMetPop124700_4505.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0502.
HOGENOM	HOG000239957.
KO	K01012.
OMA	MKETKQD.
ProtClustDB	CLSK2331944.
Enzyme and pathway databases
BioCyc	MPOP441620:GHMI-4578-MONOMER.
UniPathway	UPA00078; UER00162.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01694. BioB.
InterPro	IPR013785. Aldolase_TIM. IPR010722. Biotin/thiamin_synth-assoc. IPR002684. Biotin_synth/BioAB. IPR024177. Biotin_synthase. IPR006638. Elp3/MiaB/NifB. IPR007197. rSAM. [Graphical view]
Pfam	PF06968. BATS. 1 hit. PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF001619. Biotin_synth. 1 hit.
SMART	SM00876. BATS. 1 hit. SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00433. bioB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOB_METPB

Accession

Primary (citable) accession number: B1ZFX7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	May 20, 2008
Last modified:	May 1, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program