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Protein

Light-independent protochlorophyllide reductase iron-sulfur ATP-binding protein

Gene

bchL

Organism
Methylobacterium populi (strain ATCC BAA-705 / NCIMB 13946 / BJ001)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Component of the dark-operative protochlorophyllide reductase (DPOR) that uses Mg-ATP and reduced ferredoxin to reduce ring D of protochlorophyllide (Pchlide) to form chlorophyllide a (Chlide). This reaction is light-independent. The L component serves as a unique electron donor to the NB-component of the complex, and binds Mg-ATP.UniRule annotation

Catalytic activityi

Protochlorophyllide a + reduced ferredoxin + 2 ATP + 2 H2O = chlorophyllide a + oxidized ferredoxin + 2 ADP + 2 phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per dimer.UniRule annotation

Pathwayi: bacteriochlorophyll biosynthesis (light-independent)

This protein is involved in the pathway bacteriochlorophyll biosynthesis (light-independent), which is part of Porphyrin-containing compound metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway bacteriochlorophyll biosynthesis (light-independent) and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi45 – 451MagnesiumUniRule annotation
Binding sitei70 – 701ATPUniRule annotation
Metal bindingi126 – 1261Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation
Metal bindingi160 – 1601Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi41 – 466ATPUniRule annotation
Nucleotide bindingi211 – 2122ATPUniRule annotation
Nucleotide bindingi235 – 2373ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Bacteriochlorophyll biosynthesis, Chlorophyll biosynthesis, Photosynthesis

Keywords - Ligandi

4Fe-4S, ATP-binding, Iron, Iron-sulfur, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMPOP441620:GHMI-5431-MONOMER.
UniPathwayiUPA00671.

Names & Taxonomyi

Protein namesi
Recommended name:
Light-independent protochlorophyllide reductase iron-sulfur ATP-binding proteinUniRule annotation (EC:1.3.7.7UniRule annotation)
Short name:
DPOR subunit LUniRule annotation
Short name:
LI-POR subunit LUniRule annotation
Gene namesi
Name:bchLUniRule annotation
Ordered Locus Names:Mpop_5358
OrganismiMethylobacterium populi (strain ATCC BAA-705 / NCIMB 13946 / BJ001)
Taxonomic identifieri441620 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi
  • UP000007136 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 297297Light-independent protochlorophyllide reductase iron-sulfur ATP-binding proteinPRO_1000133440Add
BLAST

Interactioni

Subunit structurei

Homodimer. Protochlorophyllide reductase is composed of three subunits; BchL, BchN and BchB.UniRule annotation

Protein-protein interaction databases

STRINGi441620.Mpop_5358.

Structurei

3D structure databases

ProteinModelPortaliB1ZBP2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NifH/BchL/ChlL family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DSM. Bacteria.
COG1348. LUCA.
HOGENOMiHOG000228825.
KOiK04037.
OMAiTSCNISV.
OrthoDBiEOG69SK8Q.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00355. ChlL_BchL.
InterProiIPR030655. NifH/chlL_CS.
IPR000392. Nitogenase_NifH/Reductase_ChlL.
IPR027417. P-loop_NTPase.
IPR005971. Protochlorophyllide_ATP-bd.
[Graphical view]
PIRSFiPIRSF000363. Nitrogenase_iron. 1 hit.
PRINTSiPR00091. NITROGNASEII.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01281. DPOR_bchL. 1 hit.
PROSITEiPS00746. NIFH_FRXC_1. 1 hit.
PS00692. NIFH_FRXC_2. 1 hit.
PS51026. NIFH_FRXC_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1ZBP2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIAIRNPVP IRREEGSLQV ALDPADRIET AKVFAVYGKG GIGKSTTSSN
60 70 80 90 100
LSVAFSKLGK RVLQIGCDPK HDSTFTLTKR LAPTVIDALE SVNFHSEELR
110 120 130 140 150
PEDFVVEGFN GVKCVEAGGP PAGTGCGGYV VGQTVKLLKE HHLLEDTDVV
160 170 180 190 200
VFDVLGDVVC GGFASPLQHA DRALIVTAND FDSIFAMNRI VAAIHSKSKN
210 220 230 240 250
YGVRLGGVIA NRSAKTDEID RFNAAVGLRR LAHFPDLDVV RRSRLKKSTL
260 270 280 290
FEMEPSPELK AVTDEYMQLA ETLWAGADPC EAVPMKDRDL FEFLGFD
Length:297
Mass (Da):32,303
Last modified:May 20, 2008 - v1
Checksum:i7C5408A88F9B630D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001029 Genomic DNA. Translation: ACB83450.1.
RefSeqiWP_012457046.1. NC_010725.1.

Genome annotation databases

EnsemblBacteriaiACB83450; ACB83450; Mpop_5358.
KEGGimpo:Mpop_5358.
PATRICi22567996. VBIMetPop124700_5387.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001029 Genomic DNA. Translation: ACB83450.1.
RefSeqiWP_012457046.1. NC_010725.1.

3D structure databases

ProteinModelPortaliB1ZBP2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi441620.Mpop_5358.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACB83450; ACB83450; Mpop_5358.
KEGGimpo:Mpop_5358.
PATRICi22567996. VBIMetPop124700_5387.

Phylogenomic databases

eggNOGiENOG4105DSM. Bacteria.
COG1348. LUCA.
HOGENOMiHOG000228825.
KOiK04037.
OMAiTSCNISV.
OrthoDBiEOG69SK8Q.

Enzyme and pathway databases

UniPathwayiUPA00671.
BioCyciMPOP441620:GHMI-5431-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00355. ChlL_BchL.
InterProiIPR030655. NifH/chlL_CS.
IPR000392. Nitogenase_NifH/Reductase_ChlL.
IPR027417. P-loop_NTPase.
IPR005971. Protochlorophyllide_ATP-bd.
[Graphical view]
PIRSFiPIRSF000363. Nitrogenase_iron. 1 hit.
PRINTSiPR00091. NITROGNASEII.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01281. DPOR_bchL. 1 hit.
PROSITEiPS00746. NIFH_FRXC_1. 1 hit.
PS00692. NIFH_FRXC_2. 1 hit.
PS51026. NIFH_FRXC_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome of Methylobacterium populi BJ001."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.
    , Kyrpides N., Mikhailova N., Marx C., Richardson P.
    Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-705 / NCIMB 13946 / BJ001.

Entry informationi

Entry nameiBCHL_METPB
AccessioniPrimary (citable) accession number: B1ZBP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 20, 2008
Last modified: November 11, 2015
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.