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Protein

Glutamate--tRNA ligase 2

Gene

gltX2

Organism
Methylobacterium populi (strain ATCC BAA-705 / NCIMB 13946 / BJ001)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei245ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase 2UniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetase 2UniRule annotation
Short name:
GluRS 2UniRule annotation
Gene namesi
Name:gltX2UniRule annotation
Ordered Locus Names:Mpop_5295
OrganismiMethylobacterium populi (strain ATCC BAA-705 / NCIMB 13946 / BJ001)
Taxonomic identifieri441620 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi
  • UP000007136 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003677111 – 449Glutamate--tRNA ligase 2Add BLAST449

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi441620.Mpop_5295

Structurei

3D structure databases

ProteinModelPortaliB1ZAR5
SMRiB1ZAR5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi11 – 21"HIGH" regionUniRule annotationAdd BLAST11
Motifi242 – 246"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252721
KOiK01885
OMAiLYPCYET
OrthoDBiPOG091H021W

Family and domain databases

Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

B1ZAR5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASPLVRFA PSPTGFLHIG NARPALLNAL FARRAGGRFL LRLDDTDRER
60 70 80 90 100
STEEFASAVA EDLGWLGITP DLFFRQSERT ALYDTAAERL KAAGRLYPCY
110 120 130 140 150
ETPEELDRRR KRQLGRGLPP IYDRAALALS EADRAALEAE GRRPHWRFKL
160 170 180 190 200
DHRVVAWNDL VRGESHVDCA SLSDPVLVRA DGSYLYTLPS VVDDAEVGVT
210 220 230 240 250
DVIRGEDHVT NTGVQVQLFE ALGAAIPAFG HHNLLTTADG EGLSKRLGHL
260 270 280 290 300
SLRSLREAGY EPAAVRSLAV LTGSAEAVRP VASLDELASL VDLAHLSRAP
310 320 330 340 350
ARFDPAELDG MNARLVHEMP LDAVRERLAA LGVPAEAADA FWAAVRANLG
360 370 380 390 400
RVAEAADWWR VVAGPVTPVV SEPDFIARAA RLLPEAPFDA GTWKAWTDAV
410 420 430 440
KAETGAKGRA LFMPLRLALT GLDHGPDLSA LLPLIGRERA ARRLAGETA
Length:449
Mass (Da):48,657
Last modified:May 20, 2008 - v1
Checksum:i39BFFD5747A30529
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001029 Genomic DNA Translation: ACB83388.1
RefSeqiWP_012456984.1, NC_010725.1

Genome annotation databases

EnsemblBacteriaiACB83388; ACB83388; Mpop_5295
GeneIDi32085615
KEGGimpo:Mpop_5295

Similar proteinsi

Entry informationi

Entry nameiSYE2_METPB
AccessioniPrimary (citable) accession number: B1ZAR5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 20, 2008
Last modified: April 25, 2018
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health