Reviewed, UniProtKB/Swiss-Prot B1Z9T1 (SYE1_METPB)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase 1
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase 1
      Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	Mpop_5223

Organism

Methylobacterium populi (strain ATCC BAA-705 / NCIMB 13946 / BJ001) [Complete proteome] [HAMAP]

Taxonomic identifier

441620 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	474 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 474	474	Glutamyl-tRNA synthetase 1 HAMAP MF_00022		PRO_0000367710
Regions
	Motif	11 – 21	11	"HIGH" region HAMAP MF_00022
	Motif	240 – 244	5	"KMSKS" region HAMAP MF_00022
Sites
	Binding site	243	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1Z9T1-1 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 0E0BF76D8FA85ED4	FASTA	474	52,080
	10 20 30 40 50 60 MSSPVVTRFA PSPTGFLHIG GARTALFNWL YARRFGGRML LRIEDTDRER STQAAIDAIL 70 80 90 100 110 120 DGMRWLGLDW DGEVIYQFAR AERHREVAES LLASGNAYHC YASAEELAQM RETARAEGRA 130 140 150 160 170 180 PRYDGRWRDR DPSEAPPGVK PVIRLRAPTE GETVVEDAVQ GRVTWANRDL DDLVLLRSDG 190 200 210 220 230 240 TPTYMLAVVV DDHDMGVTQV IRGDDHLTNA ARQSQIYRAL GWDVPAMAHI PLIHGADGAK 250 260 270 280 290 300 LSKRHGALGV EEYRDRGYLP AALRNYLVRL GWSHGDQEVF STEEMIAAFD LKAIGRSAAR 310 320 330 340 350 360 FDFTKLESLN GLYIRNSADS VLVDAIEVIL PARGPERGLP TSLTPDLREK LLSAMPGLKE 370 380 390 400 410 420 RAKTLVELLD SAYYLYAPRP LALDAKAEGL LAGEAPERLR ALLPALEALP EWSAASTEAA 430 440 450 460 470 VRGFAESQGV KLGQVAQPLR AALTGRATSP PVFDVMAVLG RDEALARLQD RLAA

References

[1]

"Complete sequence of chromosome of Methylobacterium populi BJ001." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Marx C., Richardson P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001029 Genomic DNA. Translation: ACB83317.1.
RefSeq	YP_001927852.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6309221.
GenomeReviews	Gene locus Mpop_5223 in contig CP001029_GR.
KEGG	mpo:Mpop_5223.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	MAHIPLI.
Family and domain databases
HAMAP	MF_00022. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_METPB

Accession

Primary (citable) accession number: B1Z9T1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 20, 2008
Last modified:	November 3, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)