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Protein

Catalase-peroxidase 2

Gene

katG2

Organism
Burkholderia ambifaria (strain MC40-6)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity.UniRule annotation

Catalytic activityi

Donor + H2O2 = oxidized donor + 2 H2O.UniRule annotation
2 H2O2 = O2 + 2 H2O.UniRule annotation

Cofactori

heme bUniRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per dimer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei104 – 1041Transition state stabilizerUniRule annotation
Active sitei108 – 1081Proton acceptorUniRule annotation
Metal bindingi269 – 2691Iron (heme axial ligand)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciBAMB398577:GH38-5708-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Catalase-peroxidase 2UniRule annotation (EC:1.11.1.21UniRule annotation)
Short name:
CP 2UniRule annotation
Alternative name(s):
Peroxidase/catalase 2UniRule annotation
Gene namesi
Name:katG2UniRule annotation
Ordered Locus Names:BamMC406_5625
OrganismiBurkholderia ambifaria (strain MC40-6)
Taxonomic identifieri398577 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
Proteomesi
  • UP000001680 Componenti: Chromosome 3

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828UniRule annotationAdd
BLAST
Chaini29 – 741713Catalase-peroxidase 2PRO_5000334004Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki107 ↔ 228Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-254)UniRule annotation
Cross-linki228 ↔ 254Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-107)UniRule annotation

Post-translational modificationi

The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme.UniRule annotation

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB1Z5M2.
SMRiB1Z5M2. Positions 34-738.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Peroxidase/catalase subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000218110.
KOiK03782.
OMAiMSTRWEK.
OrthoDBiEOG6RRKKM.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B1Z5M2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQKKRIGKSV VAALAIIAMS AGTVAAWADG APRTNDFWWP ERLDLSPLRQ
60 70 80 90 100
HDVESNPYGK DFDYAQAFNK LDIEAVKKDI RATLTTSQDW WPADYGNYGP
110 120 130 140 150
FFIRMAWHGA GTYRTYDGRG GAGGAQQRFE PLNSWPDNAN LDKARRLLWP
160 170 180 190 200
IKKKYGQNIS WGDLMVLTGN VALESMGFQT FGFGGGREDD WQSDLVYWGA
210 220 230 240 250
GTKFMSDNRD KNGKLEKPLA ATQMGLIYVN PEGPNGNPDP VAAAKDIREA
260 270 280 290 300
FGRMAMNDEE TLALIAGGHT FGKAHGAASP DKCVGAAPAG AGVEAQGLGW
310 320 330 340 350
ANKCGTGKGA DTITSGLEGA WSVDPVHFTM QYLDNLLEHD WVLTKSPAGA
360 370 380 390 400
HQWMPKDAQD IVPDAHDPSK RHPLMMFTTD IALKVDPAYS AIAKRFQAHP
410 420 430 440 450
EEFKLAFAKA WFKLTHRDLG PKARYLGKDV PKVDLIWQDP LPVAGYQMIG
460 470 480 490 500
DADIAELKRR ILASGVPKSE LIKTAWASAA SFRATDYRGG ANGARIRLAP
510 520 530 540 550
ENAWAVNDPA SLSKVLKSLE DIQSGFNRNR TDGKQVSLAD LIVLGGSAAV
560 570 580 590 600
EDAARKAGYD VKVPFSPGRV DATQAQTDVA SFAVLEPTSD GFRNYYRKSN
610 620 630 640 650
ERSPAELMVD RASKLDLSVP EMTVLVGGLR ALDANAGHSR LGVLTNRPGT
660 670 680 690 700
LSNDFFVNLL DMSTQWTKSS SADGTYEGRD RKTGALKWTA SPVDLVFGSS
710 720 730 740
SELRAVAEVY ASDDAHEKFV RDFVHAWTKV MNLDRFDLKR S
Length:741
Mass (Da):80,998
Last modified:May 20, 2008 - v1
Checksum:i67DA44587234D352
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001027 Genomic DNA. Translation: ACB68068.1.
RefSeqiWP_012372008.1. NC_010557.1.

Genome annotation databases

EnsemblBacteriaiACB68068; ACB68068; BamMC406_5625.
KEGGibac:BamMC406_5625.
PATRICi19041591. VBIBurAmb82852_6076.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001027 Genomic DNA. Translation: ACB68068.1.
RefSeqiWP_012372008.1. NC_010557.1.

3D structure databases

ProteinModelPortaliB1Z5M2.
SMRiB1Z5M2. Positions 34-738.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACB68068; ACB68068; BamMC406_5625.
KEGGibac:BamMC406_5625.
PATRICi19041591. VBIBurAmb82852_6076.

Phylogenomic databases

HOGENOMiHOG000218110.
KOiK03782.
OMAiMSTRWEK.
OrthoDBiEOG6RRKKM.

Enzyme and pathway databases

BioCyciBAMB398577:GH38-5708-MONOMER.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome 3 of Burkholderia ambifaria MC40-6."
    Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A.
    , Ramette A., Konstantinidis K., Tiedje J., Richardson P.
    Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MC40-6.

Entry informationi

Entry nameiKATG2_BURA4
AccessioniPrimary (citable) accession number: B1Z5M2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: May 20, 2008
Last modified: December 9, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.