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Protein

Acetyl-coenzyme A synthetase

Gene

acsA

Organism
Burkholderia ambifaria (strain MC40-6)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.UniRule annotation

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei321 – 3211Coenzyme AUniRule annotation
Binding sitei516 – 5161ATPUniRule annotation
Binding sitei532 – 5321ATPUniRule annotation
Binding sitei540 – 5401Coenzyme A; via carbonyl oxygenUniRule annotation
Binding sitei543 – 5431ATPUniRule annotation
Metal bindingi554 – 5541Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi556 – 5561Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi559 – 5591Magnesium; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi401 – 4033ATPUniRule annotation
Nucleotide bindingi425 – 4306ATPUniRule annotation

GO - Molecular functioni

  1. acetate-CoA ligase activity Source: UniProtKB-HAMAP
  2. AMP binding Source: InterPro
  3. ATP binding Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. acetyl-CoA biosynthetic process from acetate Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciBAMB398577:GH38-5979-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
Short name:
AcCoA synthetaseUniRule annotation
Short name:
AcsUniRule annotation
Alternative name(s):
Acetate--CoA ligaseUniRule annotation
Acyl-activating enzymeUniRule annotation
Gene namesi
Name:acsAUniRule annotation
Ordered Locus Names:BamMC406_5891Imported
OrganismiBurkholderia ambifaria (strain MC40-6)Imported
Taxonomic identifieri398577 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
ProteomesiUP000001680 Componenti: Chromosome 3

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei629 – 6291N6-acetyllysineUniRule annotation

Post-translational modificationi

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.UniRule annotation

Keywords - PTMi

AcetylationUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi398577.BamMC406_5891.

Structurei

3D structure databases

ProteinModelPortaliB1Z3L5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni201 – 2044Coenzyme A bindingUniRule annotation

Sequence similaritiesi

Belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0365.
HOGENOMiHOG000229981.
KOiK01895.
OMAiKGKPYML.
OrthoDBiEOG68WR2H.

Family and domain databases

HAMAPiMF_01123. Ac_CoA_synth.
InterProiIPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1Z3L5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENTLTAIVS ILQERRIFEP TADTRERATI SGMPAYQALA AEAERDYEGF
60 70 80 90 100
WARLAREGLS WHKPFTKVLD ESDAPFYKWF DDGELNASYN CLDRHVEAGN
110 120 130 140 150
GERVAVIFEA DDGTVTRVTY ADLLARVSRF ANALKKRGIA KGDRVVIYMP
160 170 180 190 200
MSIEGIVAMQ ACARIGATHS VVFGGFSAKS LNERLVDVGA VALITADEQA
210 220 230 240 250
RGGKTLPLKS IADEAIAMGG CEAVKSVIVY RRTGGKVDWH AGRDLWMHEL
260 270 280 290 300
AHAESDTCAP EWVGAEHPLF ILYTSGSTGK PKGVQHSTGG YLLWAAQTMK
310 320 330 340 350
WTFDWKPTDV FWCTADIGWV TGHTYITYGP LACGGTQVVF EGVPTYPDAG
360 370 380 390 400
RFWKMIADHK VTVFYTAPTA IRSLIKAAEA DDKVHPKSYD LSSLRIIGTV
410 420 430 440 450
GEPINPEAWM WYHKHVGQER CPIVDTWWQT ETGGHMITPL PGATPTVPGS
460 470 480 490 500
CTLPLPGIMA AVVDETGQDV PNGQGGILVV KRPWPAMIRT IWGDPERFKK
510 520 530 540 550
SYYPEELGGR LYLAGDGTVR DKDTGYFTIM GRIDDVLNVS GHRLGTMEIE
560 570 580 590 600
SALVSHELVA EAAVVGRPDD TTGEAVVAFV VLKRSRPEGE EAAALAKTLR
610 620 630 640 650
DWVGKQIGPI AKPKDIRFGD NLPKTRSGKI MRRLLRSLAK GEAITQDTST
660
LENPAILDQL GQSL
Length:664
Mass (Da):72,777
Last modified:May 20, 2008 - v1
Checksum:i208A8B2F4243E6FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001027 Genomic DNA. Translation: ACB68328.1.
RefSeqiYP_001815881.1. NC_010557.1.

Genome annotation databases

EnsemblBacteriaiACB68328; ACB68328; BamMC406_5891.
KEGGibac:BamMC406_5891.
PATRICi19042153. VBIBurAmb82852_6352.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001027 Genomic DNA. Translation: ACB68328.1.
RefSeqiYP_001815881.1. NC_010557.1.

3D structure databases

ProteinModelPortaliB1Z3L5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi398577.BamMC406_5891.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACB68328; ACB68328; BamMC406_5891.
KEGGibac:BamMC406_5891.
PATRICi19042153. VBIBurAmb82852_6352.

Phylogenomic databases

eggNOGiCOG0365.
HOGENOMiHOG000229981.
KOiK01895.
OMAiKGKPYML.
OrthoDBiEOG68WR2H.

Enzyme and pathway databases

BioCyciBAMB398577:GH38-5979-MONOMER.

Family and domain databases

HAMAPiMF_01123. Ac_CoA_synth.
InterProiIPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome 3 of Burkholderia ambifaria MC40-6."
    Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A.
    , Ramette A., Konstantinidis K., Tiedje J., Richardson P.
    Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MC40-6Imported.

Entry informationi

Entry nameiB1Z3L5_BURA4
AccessioniPrimary (citable) accession number: B1Z3L5
Entry historyi
Integrated into UniProtKB/TrEMBL: May 20, 2008
Last sequence update: May 20, 2008
Last modified: April 1, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.