Reviewed, UniProtKB/Swiss-Prot B1Z1G4 (GCP_BURA4)

Names and origin

Protein names

Recommended name:
    Probable O-sialoglycoprotein endopeptidase
      Short name=Glycoprotease
    EC=3.4.24.57

Gene names

Name:	gcp
Ordered Locus Names:	BamMC406_3778

Organism

Burkholderia ambifaria (strain MC40-6) [Complete proteome] [HAMAP]

Taxonomic identifier

398577 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of O-sialoglycoproteins; cleaves 31-Arg-|-Asp-32 bond in glycophorin A. Does not cleave unglycosylated proteins, desialylated glycoproteins or glycoproteins that are only N-glycosylated. HAMAP MF_01445
Cofactor	Zinc By similarity. HAMAP MF_01445
Sequence similarities	Belongs to the peptidase M22 family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	Probable O-sialoglycoprotein endopeptidase HAMAP MF_01445		PRO_1000145954
Sites
	Metal binding	111	1	Zinc Potential
	Metal binding	115	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	B1Z1G4-1 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 2C57FD7D60721914	FASTA	346	36,554
	10 20 30 40 50 60 MLVLGIESSC DETGLALYDT QRGLLAHALH SQIAMHREYG GVVPELASRD HIRRALPLLE 70 80 90 100 110 120 EVMAQSGTHR DDIDAIAFTQ GPGLAGALLV GASIANALAL AWNKPTVGIH HLEGHLLSPL 130 140 150 160 170 180 LVDEPPPFPF IALLVSGGHT QLMRVTDVGV YETLGETLDD AAGEAFDKTA KLIGLGYPGG 190 200 210 220 230 240 PEVSKLAETG TPGAVVLPRP MLHSGDLDFS FSGLKTAVLT QMKKFEAAKL ESEALERAKA 250 260 270 280 290 300 DLARGFVDAA VDVLVAKSLA ALKQTKLKRL VVAGGVGANR QLRAALSAAA AKRGFDVHYP 310 320 330 340 DLALCTDNGA MIALAGALRL GRWPEQANAD YAFTVKPRWD LASLAR

References

[1]

"Complete sequence of chromosome 2 of Burkholderia ambifaria MC40-6." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A. , Ramette A., Konstantinidis K., Tiedje J., Richardson P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001026 Genomic DNA. Translation: ACB66245.1.
RefSeq	YP_001810461.1.
3D structure databases
SMR	B1Z1G4. Positions 1-343.
ModBase	Search...
Protein family/group databases
MEROPS	M22.001.
Genome annotation databases
GeneID	6181251.
GenomeReviews	Gene locus BamMC406_3778 in contig CP001026_GR.
KEGG	bac:BamMC406_3778.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG304663.
OMA	CKRALKQ.
Family and domain databases
HAMAP	MF_01445. Glycoptase_bact. [Tree]
InterPro	IPR009180. Pept_M22_O-sialoglycoprot. IPR000905. Peptidase_M22. IPR017861. Peptidase_M22_subgr. [Graphical view]
PANTHER	PTHR11735. Pept_M22_Osialgl. 1 hit.
Pfam	PF00814. Peptidase_M22. 1 hit. [Graphical view]
PRINTS	PR00789. OSIALOPTASE.
TIGRFAMs	TIGR00329. gcp. 1 hit.
PROSITE	PS01016. GLYCOPROTEASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCP_BURA4

Accession

Primary (citable) accession number: B1Z1G4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	May 20, 2008
Last modified:	February 9, 2010
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)