B1Z0R8 (METE_BURA4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	BamMC406_5175

Organism

Burkholderia ambifaria (strain MC40-6) [Complete proteome] [HAMAP]

Taxonomic identifier

398577 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	764 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 764	764	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000097817
Sites
	Metal binding	650	1	Zinc By similarity
	Metal binding	652	1	Zinc By similarity
	Metal binding	735	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1Z0R8 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: A7B237B34BE06444	FASTA	764	85,176
	10 20 30 40 50 60 MVTTHNLGFP RIGAQRELKF GLERYWKGES SRDELKALGA ELRERHWNDQ RDLDLAPIGD 70 80 90 100 110 120 FSFYDQVLDM SFTLGNLPKR VQGFHGDVLD NYFRVARGRS AQAADEHATC CGGVSAGEMT 130 140 150 160 170 180 KWFDTNYHYI VPEFHADTNF SLDPSCLLQQ LAEARALGVA GKPVILGPVT YLWLGKAKDD 190 200 210 220 230 240 SDRLALLPKL LPVYGALLDT LTAQGVEWVQ IDEPILVTEL DAEWRQALRT AYAALETRRI 250 260 270 280 290 300 KVLLATYFGA LGDNLTLAAS LPVDGLHVDA INARDEVDAL VRELPADRVL SVGAINGRNI 310 320 330 340 350 360 WKTDLNATLD WLEPLAKQLG ERLWIAPSCS LLHVPVDLAS EQKLDPEIRS WLAFALQKLD 370 380 390 400 410 420 ELNVLATALN EGRDKVADAL AANAAAIDSR RRSPRVNNPA VKAALARIDA QLGNRASPYT 430 440 450 460 470 480 QRAPKQSARL NLPAFPTTTI GSFPQTADIR QARRQFKSGA LDEAGYRAAM QAEIERSVRE 490 500 510 520 530 540 QESLELDVLV HGEAERNDMV EYFGEQLDGY AFSQFGWVQS YGSRCVKPPI LFGDISRPKA 550 560 570 580 590 600 MTVEWITYAQ SLTNKPMKGM LTGPVTILNW SFVRDDQPRS VSCYQLALAI RDEVLDLEKA 610 620 630 640 650 660 GVRVIQIDEA ALREGLPLRR AQWGEYLKWA VEAFRITANG VQDDTQIHTH MCYSEFNDII 670 680 690 700 710 720 ASIADMDADV ITIETSRSDM ELLDAFDSFR YPNEIGPGVY DIHSPNIPTQ DHIVGLMKKA 730 740 750 760 AERIPAERLW VNPDCGLKTR QWAEVIPALT NMVAAAKTLR NQVQ

References

[1]

"Complete sequence of chromosome 2 of Burkholderia ambifaria MC40-6." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A. , Ramette A., Konstantinidis K., Tiedje J., Richardson P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC40-6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001026 Genomic DNA. Translation: ACB67620.1.
RefSeq	YP_001811836.1. NC_010552.1.
3D structure databases
ProteinModelPortal	B1Z0R8.
SMR	B1Z0R8. Positions 3-762.
ModBase	Search...
Protein-protein interaction databases
STRING	B1Z0R8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6180106.
GenomeReviews	Gene locus BamMC406_5175 in contig CP001026_GR.
KEGG	bac:BamMC406_5175.
PATRIC	19040181. VBIBurAmb82852_5377.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287495.
OMA	RNIWRAN.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	BAMB398577:BAMMC406_5175-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BURA4

Accession

Primary (citable) accession number: B1Z0R8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program