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B1YWT6 (B1YWT6_BURA4) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxykinase [GTP] HAMAP-Rule MF_00452

Short name=PEP carboxykinase HAMAP-Rule MF_00452
Short name=PEPCK HAMAP-Rule MF_00452
EC=4.1.1.32 HAMAP-Rule MF_00452
Alternative name(s):
Phosphoenolpyruvate carboxylase HAMAP-Rule MF_00452
Gene names
Name:pckG HAMAP-Rule MF_00452
Ordered Locus Names:BamMC406_4337 EMBL ACB66798.1
OrganismBurkholderia ambifaria (strain MC40-6) [Complete proteome] [HAMAP] EMBL ACB66798.1
Taxonomic identifier398577 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex

Protein attributes

Sequence length621 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity. HAMAP-Rule MF_00452

Catalytic activity

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2. HAMAP-Rule MF_00452

Cofactor

Binds 1 manganese ion per subunit By similarity. HAMAP-Rule MF_00452

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00452

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00452

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00452.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. HAMAP-Rule MF_00452

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding279 – 2846GTP By similarity HAMAP-Rule MF_00452
Nucleotide binding529 – 5324GTP By similarity HAMAP-Rule MF_00452
Region399 – 4013Substrate binding By similarity HAMAP-Rule MF_00452

Sites

Active site2801 By similarity HAMAP-Rule MF_00452
Metal binding2361Manganese By similarity HAMAP-Rule MF_00452
Metal binding2561Manganese; via tele nitrogen By similarity HAMAP-Rule MF_00452
Metal binding3051Manganese By similarity HAMAP-Rule MF_00452
Binding site881Substrate By similarity HAMAP-Rule MF_00452
Binding site2291Substrate; via amide nitrogen By similarity HAMAP-Rule MF_00452
Binding site2361Substrate By similarity HAMAP-Rule MF_00452
Binding site2781Substrate By similarity HAMAP-Rule MF_00452
Binding site4011GTP By similarity HAMAP-Rule MF_00452
Binding site4321GTP By similarity HAMAP-Rule MF_00452

Sequences

Sequence LengthMass (Da)Tools
B1YWT6 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: 1406681699E21CD7

FASTA62168,563
        10         20         30         40         50         60 
MSHNTMAEAT HSPLIAPPAF VKHRKLIEWV SRIAALTEPE RVVWCDGSQE EYDALCQAMV 

        70         80         90        100        110        120 
DQGTLTRLNP EKRPNSYLAQ SDPSDVARVE DRTFICTPSP DDVGPTNNWI APEEMRETLN 

       130        140        150        160        170        180 
GLFRGSMRGR TLYVVPFSMG PLGSPIAHIG VELSDSPYVV VNMRIMTRMG REVYDVLGED 

       190        200        210        220        230        240 
GDFVPCVHSV GHPLDAGQKD VRWPCNPVKY IVHFPETREI WSFGSGYGGN ALLGKKCFAL 

       250        260        270        280        290        300 
RIASTMGRDQ GWLAEHMLIL GVTSPAGKKY HVAAAFPSAC GKTNFAMLIP PQGFDGWKVT 

       310        320        330        340        350        360 
TIGDDIAWLK PGRDGRLYAI NPEAGFFGVA PGTGVKTNPN AISTLKENVI FTNVALTEDG 

       370        380        390        400        410        420 
DVWWEGLTDT PPAKLTDWQG NPWTPEIGKE TGRKAAHPNS RFTAPASQCP SIDADWENPA 

       430        440        450        460        470        480 
GVPIDAFIFG GRRSTTVPLV TEARDWVEGV YMAATMGSET TAAAVGQQGI VRRDPFAMLP 

       490        500        510        520        530        540 
FCGYNVSDYF AHWLKIGKQL EGAGAKLPKI YCVNWFRKDA NGKFVWPGFG ENMRVLKWML 

       550        560        570        580        590        600 
DRLEGNGGGG EHAFGVSPAY DDLHWEGLHF TREQFEAVTS MNADEWREEL KLHAALFDTL 

       610        620 
KLRLPTELTD TMKKIEQRLG G 

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References

[1]"Complete sequence of chromosome 2 of Burkholderia ambifaria MC40-6."
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A. expand/collapse author list , Ramette A., Konstantinidis K., Tiedje J., Richardson P.
Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MC40-6.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001026 Genomic DNA. Translation: ACB66798.1.
RefSeqYP_001811014.1. NC_010552.1.

3D structure databases

ProteinModelPortalB1YWT6.
SMRB1YWT6. Positions 20-620.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING398577.BamMC406_4337.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACB66798; ACB66798; BamMC406_4337.
GeneID6180615.
KEGGbac:BamMC406_4337.
PATRIC19038405. VBIBurAmb82852_4491.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1274.
HOGENOMHOG000191700.
KOK01596.
OMAWMRFGED.
OrthoDBEOG6MPWQS.
ProtClustDBPRK04210.

Enzyme and pathway databases

BioCycBAMB398577:GH38-4414-MONOMER.
UniPathwayUPA00138.

Family and domain databases

Gene3D3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPMF_00452. PEPCK_GTP.
InterProIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERPTHR11561. PTHR11561. 1 hit.
PfamPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMSSF68923. SSF68923. 1 hit.
PROSITEPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB1YWT6_BURA4
AccessionPrimary (citable) accession number: B1YWT6
Entry history
Integrated into UniProtKB/TrEMBL: May 20, 2008
Last sequence update: May 20, 2008
Last modified: February 19, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)