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B1YWT6

- B1YWT6_BURA4

UniProt

B1YWT6 - B1YWT6_BURA4

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Protein
Phosphoenolpyruvate carboxykinase [GTP]
Gene
pckG, BamMC406_4337
Organism
Burkholderia ambifaria (strain MC40-6)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Binds 1 manganese ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei88 – 881Substrate By similarityUniRule annotation
Binding sitei229 – 2291Substrate; via amide nitrogen By similarityUniRule annotation
Metal bindingi236 – 2361Manganese By similarityUniRule annotation
Binding sitei236 – 2361Substrate By similarityUniRule annotation
Metal bindingi256 – 2561Manganese; via tele nitrogen By similarityUniRule annotation
Binding sitei278 – 2781Substrate By similarityUniRule annotation
Active sitei280 – 2801 By similarityUniRule annotation
Metal bindingi305 – 3051Manganese By similarityUniRule annotation
Binding sitei401 – 4011GTP By similarityUniRule annotation
Binding sitei432 – 4321GTP By similarityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi279 – 2846GTP By similarityUniRule annotation
Nucleotide bindingi529 – 5324GTP By similarityUniRule annotation

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. kinase activity Source: UniProtKB-KW
  3. manganese ion binding Source: UniProtKB-HAMAP
  4. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

DecarboxylaseUniRule annotationSAAS annotations, KinaseImported, Lyase, Transferase

Keywords - Biological processi

GluconeogenesisUniRule annotation

Keywords - Ligandi

GTP-bindingUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PyruvateImported

Enzyme and pathway databases

BioCyciBAMB398577:GH38-4414-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Alternative name(s):
Phosphoenolpyruvate carboxylase
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:BamMC406_4337Imported
OrganismiBurkholderia ambifaria (strain MC40-6)Imported
Taxonomic identifieri398577 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
ProteomesiUP000001680: Chromosome 2

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi398577.BamMC406_4337.

Structurei

3D structure databases

ProteinModelPortaliB1YWT6.
SMRiB1YWT6. Positions 20-620.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni399 – 4013Substrate binding By similarityUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWDNPAGV.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1YWT6-1 [UniParc]FASTAAdd to Basket

« Hide

MSHNTMAEAT HSPLIAPPAF VKHRKLIEWV SRIAALTEPE RVVWCDGSQE    50
EYDALCQAMV DQGTLTRLNP EKRPNSYLAQ SDPSDVARVE DRTFICTPSP 100
DDVGPTNNWI APEEMRETLN GLFRGSMRGR TLYVVPFSMG PLGSPIAHIG 150
VELSDSPYVV VNMRIMTRMG REVYDVLGED GDFVPCVHSV GHPLDAGQKD 200
VRWPCNPVKY IVHFPETREI WSFGSGYGGN ALLGKKCFAL RIASTMGRDQ 250
GWLAEHMLIL GVTSPAGKKY HVAAAFPSAC GKTNFAMLIP PQGFDGWKVT 300
TIGDDIAWLK PGRDGRLYAI NPEAGFFGVA PGTGVKTNPN AISTLKENVI 350
FTNVALTEDG DVWWEGLTDT PPAKLTDWQG NPWTPEIGKE TGRKAAHPNS 400
RFTAPASQCP SIDADWENPA GVPIDAFIFG GRRSTTVPLV TEARDWVEGV 450
YMAATMGSET TAAAVGQQGI VRRDPFAMLP FCGYNVSDYF AHWLKIGKQL 500
EGAGAKLPKI YCVNWFRKDA NGKFVWPGFG ENMRVLKWML DRLEGNGGGG 550
EHAFGVSPAY DDLHWEGLHF TREQFEAVTS MNADEWREEL KLHAALFDTL 600
KLRLPTELTD TMKKIEQRLG G 621
Length:621
Mass (Da):68,563
Last modified:May 20, 2008 - v1
Checksum:i1406681699E21CD7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001026 Genomic DNA. Translation: ACB66798.1.
RefSeqiWP_012366116.1. NC_010552.1.
YP_001811014.1. NC_010552.1.

Genome annotation databases

EnsemblBacteriaiACB66798; ACB66798; BamMC406_4337.
GeneIDi6180615.
KEGGibac:BamMC406_4337.
PATRICi19038405. VBIBurAmb82852_4491.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001026 Genomic DNA. Translation: ACB66798.1 .
RefSeqi WP_012366116.1. NC_010552.1.
YP_001811014.1. NC_010552.1.

3D structure databases

ProteinModelPortali B1YWT6.
SMRi B1YWT6. Positions 20-620.
ModBasei Search...

Protein-protein interaction databases

STRINGi 398577.BamMC406_4337.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACB66798 ; ACB66798 ; BamMC406_4337 .
GeneIDi 6180615.
KEGGi bac:BamMC406_4337.
PATRICi 19038405. VBIBurAmb82852_4491.

Phylogenomic databases

eggNOGi COG1274.
HOGENOMi HOG000191700.
KOi K01596.
OMAi WDNPAGV.
OrthoDBi EOG6MPWQS.

Enzyme and pathway databases

UniPathwayi UPA00138 .
BioCyci BAMB398577:GH38-4414-MONOMER.

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome 2 of Burkholderia ambifaria MC40-6."
    Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A.
    , Ramette A., Konstantinidis K., Tiedje J., Richardson P.
    Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MC40-6.

Entry informationi

Entry nameiB1YWT6_BURA4
AccessioniPrimary (citable) accession number: B1YWT6
Entry historyi
Integrated into UniProtKB/TrEMBL: May 20, 2008
Last sequence update: May 20, 2008
Last modified: September 3, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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