Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

B1YWT6

- B1YWT6_BURA4

UniProt

B1YWT6 - B1YWT6_BURA4

Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Burkholderia ambifaria (strain MC40-6)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 43 (01 Oct 2014)
      Sequence version 1 (20 May 2008)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

    Cofactori

    Binds 1 manganese ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei88 – 881SubstrateUniRule annotation
    Binding sitei229 – 2291Substrate; via amide nitrogenUniRule annotation
    Metal bindingi236 – 2361ManganeseUniRule annotation
    Binding sitei236 – 2361SubstrateUniRule annotation
    Metal bindingi256 – 2561Manganese; via tele nitrogenUniRule annotation
    Binding sitei278 – 2781SubstrateUniRule annotation
    Active sitei280 – 2801UniRule annotation
    Metal bindingi305 – 3051ManganeseUniRule annotation
    Binding sitei401 – 4011GTPUniRule annotation
    Binding sitei432 – 4321GTPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi279 – 2846GTPUniRule annotation
    Nucleotide bindingi529 – 5324GTPUniRule annotation

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-HAMAP
    2. kinase activity Source: UniProtKB-KW
    3. manganese ion binding Source: UniProtKB-HAMAP
    4. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    DecarboxylaseUniRule annotationSAAS annotation, KinaseImported, Lyase, Transferase

    Keywords - Biological processi

    GluconeogenesisUniRule annotation

    Keywords - Ligandi

    GTP-bindingUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PyruvateImported

    Enzyme and pathway databases

    BioCyciBAMB398577:GH38-4414-MONOMER.
    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
    Short name:
    PEP carboxykinaseUniRule annotation
    Short name:
    PEPCKUniRule annotation
    Gene namesi
    Name:pckGUniRule annotation
    Ordered Locus Names:BamMC406_4337Imported
    OrganismiBurkholderia ambifaria (strain MC40-6)Imported
    Taxonomic identifieri398577 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
    ProteomesiUP000001680: Chromosome 2

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi398577.BamMC406_4337.

    Structurei

    3D structure databases

    ProteinModelPortaliB1YWT6.
    SMRiB1YWT6. Positions 20-620.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni399 – 4013Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1274.
    HOGENOMiHOG000191700.
    KOiK01596.
    OMAiWDNPAGV.
    OrthoDBiEOG6MPWQS.

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    PROSITEiPS00505. PEPCK_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B1YWT6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSHNTMAEAT HSPLIAPPAF VKHRKLIEWV SRIAALTEPE RVVWCDGSQE    50
    EYDALCQAMV DQGTLTRLNP EKRPNSYLAQ SDPSDVARVE DRTFICTPSP 100
    DDVGPTNNWI APEEMRETLN GLFRGSMRGR TLYVVPFSMG PLGSPIAHIG 150
    VELSDSPYVV VNMRIMTRMG REVYDVLGED GDFVPCVHSV GHPLDAGQKD 200
    VRWPCNPVKY IVHFPETREI WSFGSGYGGN ALLGKKCFAL RIASTMGRDQ 250
    GWLAEHMLIL GVTSPAGKKY HVAAAFPSAC GKTNFAMLIP PQGFDGWKVT 300
    TIGDDIAWLK PGRDGRLYAI NPEAGFFGVA PGTGVKTNPN AISTLKENVI 350
    FTNVALTEDG DVWWEGLTDT PPAKLTDWQG NPWTPEIGKE TGRKAAHPNS 400
    RFTAPASQCP SIDADWENPA GVPIDAFIFG GRRSTTVPLV TEARDWVEGV 450
    YMAATMGSET TAAAVGQQGI VRRDPFAMLP FCGYNVSDYF AHWLKIGKQL 500
    EGAGAKLPKI YCVNWFRKDA NGKFVWPGFG ENMRVLKWML DRLEGNGGGG 550
    EHAFGVSPAY DDLHWEGLHF TREQFEAVTS MNADEWREEL KLHAALFDTL 600
    KLRLPTELTD TMKKIEQRLG G 621
    Length:621
    Mass (Da):68,563
    Last modified:May 20, 2008 - v1
    Checksum:i1406681699E21CD7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001026 Genomic DNA. Translation: ACB66798.1.
    RefSeqiWP_012366116.1. NC_010552.1.
    YP_001811014.1. NC_010552.1.

    Genome annotation databases

    EnsemblBacteriaiACB66798; ACB66798; BamMC406_4337.
    GeneIDi6180615.
    KEGGibac:BamMC406_4337.
    PATRICi19038405. VBIBurAmb82852_4491.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001026 Genomic DNA. Translation: ACB66798.1 .
    RefSeqi WP_012366116.1. NC_010552.1.
    YP_001811014.1. NC_010552.1.

    3D structure databases

    ProteinModelPortali B1YWT6.
    SMRi B1YWT6. Positions 20-620.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 398577.BamMC406_4337.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACB66798 ; ACB66798 ; BamMC406_4337 .
    GeneIDi 6180615.
    KEGGi bac:BamMC406_4337.
    PATRICi 19038405. VBIBurAmb82852_4491.

    Phylogenomic databases

    eggNOGi COG1274.
    HOGENOMi HOG000191700.
    KOi K01596.
    OMAi WDNPAGV.
    OrthoDBi EOG6MPWQS.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .
    BioCyci BAMB398577:GH38-4414-MONOMER.

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    PROSITEi PS00505. PEPCK_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of chromosome 2 of Burkholderia ambifaria MC40-6."
      Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A.
      , Ramette A., Konstantinidis K., Tiedje J., Richardson P.
      Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MC40-6Imported.

    Entry informationi

    Entry nameiB1YWT6_BURA4
    AccessioniPrimary (citable) accession number: B1YWT6
    Entry historyi
    Integrated into UniProtKB/TrEMBL: May 20, 2008
    Last sequence update: May 20, 2008
    Last modified: October 1, 2014
    This is version 43 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3