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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Burkholderia ambifaria (strain MC40-6)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathway: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei88 – 881SubstrateUniRule annotation
Binding sitei229 – 2291Substrate; via amide nitrogenUniRule annotation
Metal bindingi236 – 2361ManganeseUniRule annotation
Binding sitei236 – 2361SubstrateUniRule annotation
Metal bindingi256 – 2561Manganese; via tele nitrogenUniRule annotation
Binding sitei278 – 2781SubstrateUniRule annotation
Active sitei280 – 2801UniRule annotation
Metal bindingi305 – 3051ManganeseUniRule annotation
Binding sitei401 – 4011GTPUniRule annotation
Binding sitei432 – 4321GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi279 – 2846GTPUniRule annotation
Nucleotide bindingi529 – 5324GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DecarboxylaseUniRule annotationSAAS annotation, KinaseImported, Lyase, Transferase

Keywords - Biological processi

GluconeogenesisUniRule annotation

Keywords - Ligandi

GTP-bindingUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PyruvateImported

Enzyme and pathway databases

BioCyciBAMB398577:GH38-4414-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:BamMC406_4337Imported
OrganismiBurkholderia ambifaria (strain MC40-6)Imported
Taxonomic identifieri398577 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
ProteomesiUP000001680 Componenti: Chromosome 2

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB1YWT6.
SMRiB1YWT6. Positions 20-620.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni399 – 4013Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1YWT6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHNTMAEAT HSPLIAPPAF VKHRKLIEWV SRIAALTEPE RVVWCDGSQE
60 70 80 90 100
EYDALCQAMV DQGTLTRLNP EKRPNSYLAQ SDPSDVARVE DRTFICTPSP
110 120 130 140 150
DDVGPTNNWI APEEMRETLN GLFRGSMRGR TLYVVPFSMG PLGSPIAHIG
160 170 180 190 200
VELSDSPYVV VNMRIMTRMG REVYDVLGED GDFVPCVHSV GHPLDAGQKD
210 220 230 240 250
VRWPCNPVKY IVHFPETREI WSFGSGYGGN ALLGKKCFAL RIASTMGRDQ
260 270 280 290 300
GWLAEHMLIL GVTSPAGKKY HVAAAFPSAC GKTNFAMLIP PQGFDGWKVT
310 320 330 340 350
TIGDDIAWLK PGRDGRLYAI NPEAGFFGVA PGTGVKTNPN AISTLKENVI
360 370 380 390 400
FTNVALTEDG DVWWEGLTDT PPAKLTDWQG NPWTPEIGKE TGRKAAHPNS
410 420 430 440 450
RFTAPASQCP SIDADWENPA GVPIDAFIFG GRRSTTVPLV TEARDWVEGV
460 470 480 490 500
YMAATMGSET TAAAVGQQGI VRRDPFAMLP FCGYNVSDYF AHWLKIGKQL
510 520 530 540 550
EGAGAKLPKI YCVNWFRKDA NGKFVWPGFG ENMRVLKWML DRLEGNGGGG
560 570 580 590 600
EHAFGVSPAY DDLHWEGLHF TREQFEAVTS MNADEWREEL KLHAALFDTL
610 620
KLRLPTELTD TMKKIEQRLG G
Length:621
Mass (Da):68,563
Last modified:May 20, 2008 - v1
Checksum:i1406681699E21CD7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001026 Genomic DNA. Translation: ACB66798.1.
RefSeqiWP_012366116.1. NC_010552.1.
YP_001811014.1. NC_010552.1.

Genome annotation databases

EnsemblBacteriaiACB66798; ACB66798; BamMC406_4337.
KEGGibac:BamMC406_4337.
PATRICi19038405. VBIBurAmb82852_4491.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001026 Genomic DNA. Translation: ACB66798.1.
RefSeqiWP_012366116.1. NC_010552.1.
YP_001811014.1. NC_010552.1.

3D structure databases

ProteinModelPortaliB1YWT6.
SMRiB1YWT6. Positions 20-620.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACB66798; ACB66798; BamMC406_4337.
KEGGibac:BamMC406_4337.
PATRICi19038405. VBIBurAmb82852_4491.

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Enzyme and pathway databases

UniPathwayiUPA00138.
BioCyciBAMB398577:GH38-4414-MONOMER.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome 2 of Burkholderia ambifaria MC40-6."
    Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A.
    , Ramette A., Konstantinidis K., Tiedje J., Richardson P.
    Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MC40-6Imported.

Entry informationi

Entry nameiB1YWT6_BURA4
AccessioniPrimary (citable) accession number: B1YWT6
Entry historyi
Integrated into UniProtKB/TrEMBL: May 20, 2008
Last sequence update: May 20, 2008
Last modified: June 24, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.