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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Burkholderia ambifaria (strain MC40-6)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei88SubstrateUniRule annotation1
Metal bindingi236ManganeseUniRule annotation1
Metal bindingi256Manganese; via tele nitrogenUniRule annotation1
Binding sitei278SubstrateUniRule annotation1
Active sitei280UniRule annotation1
Metal bindingi305ManganeseUniRule annotation1
Binding sitei401GTPUniRule annotation1
Binding sitei432GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi279 – 284GTPUniRule annotation6
Nucleotide bindingi529 – 532GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylaseUniRule annotationSAAS annotation, KinaseImported, Lyase, Transferase
Biological processGluconeogenesisUniRule annotation
LigandGTP-bindingUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PyruvateImported

Enzyme and pathway databases

BioCyciBAMB398577:G1GBO-4350-MONOMER
UniPathwayiUPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Alternative name(s):
GTP-dependent phosphoenolpyruvate carboxykinaseUniRule annotation
Short name:
GTP-PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:BamMC406_4337Imported
OrganismiBurkholderia ambifaria (strain MC40-6)Imported
Taxonomic identifieri398577 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
Proteomesi
  • UP000001680 Componenti: Chromosome 2

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB1YWT6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 248PEPCK_NInterPro annotationAdd BLAST221
Domaini252 – 619PEPCK_CInterPro annotationAdd BLAST368

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni227 – 229Substrate bindingUniRule annotation3
Regioni399 – 401Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000191700
KOiK01596
OMAiCWLALTD
OrthoDBiPOG091H0B4F

Family and domain databases

CDDicd00819 PEPCK_GTP, 1 hit
Gene3Di3.40.449.10, 2 hits
3.90.228.20, 2 hits
HAMAPiMF_00452 PEPCK_GTP, 1 hit
InterProiView protein in InterPro
IPR018091 PEP_carboxykin_GTP_CS
IPR013035 PEP_carboxykinase_C
IPR008209 PEP_carboxykinase_GTP
IPR035077 PEP_carboxykinase_GTP_C
IPR035078 PEP_carboxykinase_GTP_N
IPR008210 PEP_carboxykinase_N
PANTHERiPTHR11561 PTHR11561, 1 hit
PfamiView protein in Pfam
PF00821 PEPCK_C, 1 hit
PF17297 PEPCK_N, 1 hit
PIRSFiPIRSF001348 PEP_carboxykinase_GTP, 1 hit
SUPFAMiSSF68923 SSF68923, 1 hit
PROSITEiView protein in PROSITE
PS00505 PEPCK_GTP, 1 hit

Sequencei

Sequence statusi: Complete.

B1YWT6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHNTMAEAT HSPLIAPPAF VKHRKLIEWV SRIAALTEPE RVVWCDGSQE
60 70 80 90 100
EYDALCQAMV DQGTLTRLNP EKRPNSYLAQ SDPSDVARVE DRTFICTPSP
110 120 130 140 150
DDVGPTNNWI APEEMRETLN GLFRGSMRGR TLYVVPFSMG PLGSPIAHIG
160 170 180 190 200
VELSDSPYVV VNMRIMTRMG REVYDVLGED GDFVPCVHSV GHPLDAGQKD
210 220 230 240 250
VRWPCNPVKY IVHFPETREI WSFGSGYGGN ALLGKKCFAL RIASTMGRDQ
260 270 280 290 300
GWLAEHMLIL GVTSPAGKKY HVAAAFPSAC GKTNFAMLIP PQGFDGWKVT
310 320 330 340 350
TIGDDIAWLK PGRDGRLYAI NPEAGFFGVA PGTGVKTNPN AISTLKENVI
360 370 380 390 400
FTNVALTEDG DVWWEGLTDT PPAKLTDWQG NPWTPEIGKE TGRKAAHPNS
410 420 430 440 450
RFTAPASQCP SIDADWENPA GVPIDAFIFG GRRSTTVPLV TEARDWVEGV
460 470 480 490 500
YMAATMGSET TAAAVGQQGI VRRDPFAMLP FCGYNVSDYF AHWLKIGKQL
510 520 530 540 550
EGAGAKLPKI YCVNWFRKDA NGKFVWPGFG ENMRVLKWML DRLEGNGGGG
560 570 580 590 600
EHAFGVSPAY DDLHWEGLHF TREQFEAVTS MNADEWREEL KLHAALFDTL
610 620
KLRLPTELTD TMKKIEQRLG G
Length:621
Mass (Da):68,563
Last modified:May 20, 2008 - v1
Checksum:i1406681699E21CD7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001026 Genomic DNA Translation: ACB66798.1
RefSeqiWP_012366116.1, NC_010552.1

Genome annotation databases

EnsemblBacteriaiACB66798; ACB66798; BamMC406_4337
KEGGibac:BamMC406_4337

Similar proteinsi

Entry informationi

Entry nameiB1YWT6_BURA4
AccessioniPrimary (citable) accession number: B1YWT6
Entry historyiIntegrated into UniProtKB/TrEMBL: May 20, 2008
Last sequence update: May 20, 2008
Last modified: March 28, 2018
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
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Main funding by: National Institutes of Health