B1YVH2 (T23O_BURA4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan 2,3-dioxygenase
Short name=TDO
EC=1.13.11.11

Alternative name(s):
Tryptamin 2,3-dioxygenase
Tryptophan oxygenase
Short name=TO
Short name=TRPO
Tryptophan pyrrolase
Tryptophanase

Gene names

Name:	kynA
Ordered Locus Names:	BamMC406_2498

Organism

Burkholderia ambifaria (strain MC40-6) [Complete proteome] [HAMAP]

Taxonomic identifier

398577 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.
Sequence caution	The sequence ACB64976.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	Tryptophan 2,3-dioxygenase		PRO_0000360096
Regions
	Region	52 – 56	5	Substrate binding By similarity
	Region	77 – 81	5	Substrate binding By similarity
Sites
	Metal binding	266	1	Iron (heme axial ligand) By similarity
	Binding site	139	1	Substrate By similarity
	Binding site	143	1	Substrate By similarity
	Binding site	150	1	Heme By similarity
	Binding site	280	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1YVH2 [UniParc]. Last modified January 20, 2009. Version 2. Checksum: 68A0A0F5F6216E1E	FASTA	308	35,206
	10 20 30 40 50 60 MKPPGDNAPA GCPFSGARAA QPAHEAPHVP GDAAGETGWH DAQLDFSKSM SYGDYLSLNS 70 80 90 100 110 120 ILDAQHPLSP DHNEMLFIIQ HQTSELWMKL ALFELRGALD AVRGDALPPA FKMLARVSRI 130 140 150 160 170 180 LEQLVQAWNV LSTMTPSEYS AMRPYLGQSS GFQSYQYRQL EFLLGNKNVQ MLQPHAHRPD 190 200 210 220 230 240 ILEQVRATLE APSFYDEVVR LLARRGFPIA SERLERDWTQ PMRHDETVEA AWLEVYRHPQ 250 260 270 280 290 300 QHWELYEMAE ELVDLEDAFR QWRFRHVTTV ERIIGFKQGT GGTSGAPYLR KMLDVVLFPE LWHVRTTL

References

[1]

"Complete sequence of chromosome 1 of Burkholderia ambifaria MC40-6." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A. , Ramette A., Konstantinidis K., Tiedje J., Richardson P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC40-6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001025 Genomic DNA. Translation: ACB64976.1. Different initiation.
RefSeq	YP_001809192.1. NC_010551.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B1YVH2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6178500.
GenomeReviews	Gene locus BamMC406_2498 in contig CP001025_GR.
KEGG	bac:BamMC406_2498.
PATRIC	19034543. VBIBurAmb82852_2570.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG647485.
ProtClustDB	CLSK930429.
Enzyme and pathway databases
BioCyc	BAMB398577:BAMMC406_2498-MONOMER.
Family and domain databases
InterPro	IPR017485. Trp_2-3-dOase_bac. IPR004981. Trp_2_3_dOase. [Graphical view]
KO	K00453.
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
TIGRFAMs	TIGR03036. Trp_2_3_diox. 1 hit.
ProtoNet	Search...

Entry information

Entry name

T23O_BURA4

Accession

Primary (citable) accession number: B1YVH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	January 20, 2009
Last modified:	January 25, 2012
This is version 28 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program