dut

Functionⁱ

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.UniRule annotation

Catalytic activityⁱ

dUTP + H₂O = dUMP + diphosphate.UniRule annotation

Cofactorⁱ

Mg²⁺UniRule annotation

Pathwayⁱ: dUMP biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes dUMP from dCTP (dUTP route).UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:

dCTP deaminase (dcd)
Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut)

This subpathway is part of the pathway dUMP biosynthesis, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dUMP from dCTP (dUTP route), the pathway dUMP biosynthesis and in Pyrimidine metabolism.

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Binding siteⁱ	80	SubstrateUniRule annotation		1
Binding siteⁱ	94	Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation		1

GO - Molecular functionⁱ

dUTP diphosphatase activity Source: UniProtKB-EC
metal ion binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

dUMP biosynthetic process Source: UniProtKB-UniPathway
dUTP metabolic process Source: InterPro

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase
Biological process	Nucleotide metabolism
Ligand	Magnesium, Metal-binding

Enzyme and pathway databases

BioCycⁱ	BAMB398577:G1GBO-2466-MONOMER.
UniPathwayⁱ	UPA00610; UER00666.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Deoxyuridine 5'-triphosphate nucleotidohydrolaseUniRule annotation (EC:3.6.1.23UniRule annotation) Short name: dUTPaseUniRule annotation Alternative name(s): dUTP pyrophosphataseUniRule annotation
Gene namesⁱ	Name:dutUniRule annotation Ordered Locus Names:BamMC406_2433
Organismⁱ	Burkholderia ambifaria (strain MC40-6)
Taxonomic identifierⁱ	398577 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex › Burkholderia ambifaria
Proteomesⁱ	UP000001680 Componentⁱ: Chromosome 1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_1000094944	1 – 148	Deoxyuridine 5'-triphosphate nucleotidohydrolaseAdd BLAST		148

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	B1YVA7.
SMRⁱ	B1YVA7.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	67 – 69	Substrate bindingUniRule annotation		3
Regionⁱ	84 – 86	Substrate bindingUniRule annotation		3

Sequence similaritiesⁱ

Belongs to the dUTPase family.UniRule annotation

Phylogenomic databases

HOGENOMⁱ	HOG000028968.
KEGG Orthology (KO) More...KOⁱ	K01520.
OMAⁱ	GVILINH.
Database of Orthologous Groups More...OrthoDBⁱ	POG091H01I6.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd07557. trimeric_dUTPase. 1 hit.
Gene3Dⁱ	2.70.40.10. 1 hit.
HAMAPⁱ	MF_00116. dUTPase_bact. 1 hit.
InterProⁱ	View protein in InterPro IPR029054. dUTPase-like. IPR036157. dUTPase-like_sf. IPR008181. dUTPase_1. IPR033704. dUTPase_trimeric.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00692. dUTPase. 1 hit.
SUPFAMⁱ	SSF51283. SSF51283. 1 hit.
TIGRFAMsⁱ	TIGR00576. dut. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

B1YVA7-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKLDLKILDA RMRDYMPAYA TTGSAGLDLR ACLDAPVTLQ PGETTLVPTG 
        60         70         80         90        100
LAIHLADPGY AALILPRSGL GHKHGIVLGN LVGLIDSDYQ GQLMVSTWNR 
       110        120        130        140 
GQTEFVLNPF ERLAQLVIVP VVQAQFNIVD DFAQSERGEG GFGSTGRH

Length:148

Mass (Da):15,930

Last modified:May 20, 2008 - v1

Checksum:ⁱBB1275F53AB1240E

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP001025 Genomic DNA. Translation: ACB64911.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_012364518.1. NC_010551.1.

Genome annotation databases

EnsemblBacteriaⁱ	ACB64911; ACB64911; BamMC406_2433.
KEGGⁱ	bac:BamMC406_2433.

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
B1YVA7	Q0BCK5 A9AGN3 A0A1B4TZD1 J5B4B2 B9B4D9 A0A1W0ZLU4 Q1BU98 B1JXC6 A0K9T8 A0A1U9MTB7 +175	Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia cepacia (strain AMMD)) Burkholderia multivorans (strain ATCC 17616 / 249) Burkholderia multivorans Burkholderia multivorans CF2 Burkholderia multivorans CGD1 Burkholderia cenocepacia (strain AU 1054) Burkholderia cenocepacia (strain MC0-3) Burkholderia cenocepacia (strain HI2424) Burkholderia cenocepacia Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315)) And more	148	UniRef90_Q0BCK5	Cluster: Deoxyuridine 5'-triphosphate nucleotidohydrolase	186

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
B1YVA7	Q5ZSN0 A0A2J9NGI0 A0A130B645 G8UX61 A0A0W0X4I6 UPI0008634099 A0A1P8N914 A0A0A2SQC0 A0A128JBY7 Q5X242 +2755	Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) Legionella pneumophila subsp. pneumophila Legionella pneumophila Legionella pneumophila subsp. pneumophila ATCC 43290 Legionella moravica Legionella norrlandica Legionella pneumophila (strain Paris) Legionella pneumophila subsp. pascullei Legionella pneumophila (strain Lens) Legionella quateirensis And more	152	UniRef50_Q5ZSN0	Cluster: Deoxyuridine 5'-triphosphate nucleotidohydrolase	2,766

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP001025 Genomic DNA. Translation: ACB64911.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_012364518.1. NC_010551.1.

3D structure databases

ProteinModelPortalⁱ	B1YVA7.
SMRⁱ	B1YVA7.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	ACB64911; ACB64911; BamMC406_2433.
KEGGⁱ	bac:BamMC406_2433.

Phylogenomic databases

HOGENOMⁱ	HOG000028968.
KEGG Orthology (KO) More...KOⁱ	K01520.
OMAⁱ	GVILINH.
Database of Orthologous Groups More...OrthoDBⁱ	POG091H01I6.

Enzyme and pathway databases

UniPathwayⁱ	UPA00610; UER00666.
BioCycⁱ	BAMB398577:G1GBO-2466-MONOMER.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd07557. trimeric_dUTPase. 1 hit.
Gene3Dⁱ	2.70.40.10. 1 hit.
HAMAPⁱ	MF_00116. dUTPase_bact. 1 hit.
InterProⁱ	View protein in InterPro IPR029054. dUTPase-like. IPR036157. dUTPase-like_sf. IPR008181. dUTPase_1. IPR033704. dUTPase_trimeric.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00692. dUTPase. 1 hit.
SUPFAMⁱ	SSF51283. SSF51283. 1 hit.
TIGRFAMsⁱ	TIGR00576. dut. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	DUT_BURA4
Accessionⁱ	B1YVA7Primary (citable) accession number: B1YVA7
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
	Last sequence update:	May 20, 2008
	Last modified:	February 28, 2018
	This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome

UniProtKB - B1YVA7 (DUT_BURA4)

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Deoxyuridine 5'-triphosphate nucleotidohydrolase

Select a section on the left to see content.

Functionⁱ

Catalytic activityⁱ

Cofactorⁱ

Pathwayⁱ: dUMP biosynthesis

Sites

GO - Molecular functionⁱ

GO - Biological processⁱ

Keywordsⁱ

Enzyme and pathway databases

Names & Taxonomyⁱ

PTM / Processingⁱ

Molecule processing

Structureⁱ

3D structure databases

Family & Domainsⁱ

Region

Sequence similaritiesⁱ

Phylogenomic databases

Family and domain databases

Sequenceⁱ

Sequence databases

Genome annotation databases

Similar proteinsⁱ

Cross-referencesⁱ

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - B1YVA7 (DUT_BURA4)

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Deoxyuridine 5'-triphosphate nucleotidohydrolase

dut

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: dUMP biosynthesis

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Pathwayⁱ: dUMP biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ