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UniProtKB - B1YVA7 (DUT_BURA4)

UniProt

B1YVA7 - DUT_BURA4

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Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

dut

Organism
Burkholderia ambifaria (strain MC40-6)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.UniRule annotation

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: dUMP biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes dUMP from dCTP (dUTP route).UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Deoxycytidine triphosphate deaminase (dcd)
  2. Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut)
This subpathway is part of the pathway dUMP biosynthesis, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dUMP from dCTP (dUTP route), the pathway dUMP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei80 – 801SubstrateUniRule annotation
Binding sitei94 – 941Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciBAMB398577:GH38-2503-MONOMER.
UniPathwayiUPA00610; UER00666.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolaseUniRule annotation (EC:3.6.1.23UniRule annotation)
Short name:
dUTPaseUniRule annotation
Alternative name(s):
dUTP pyrophosphataseUniRule annotation
Gene namesi
Name:dutUniRule annotation
Ordered Locus Names:BamMC406_2433
OrganismiBurkholderia ambifaria (strain MC40-6)
Taxonomic identifieri398577 [NCBI]
Taxonomic lineageiBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex ›
Proteomesi
  • UP000001680 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 148148Deoxyuridine 5'-triphosphate nucleotidohydrolasePRO_1000094944Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliB1YVA7.
SMRiB1YVA7. Positions 2-132.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni67 – 693Substrate bindingUniRule annotation
Regioni84 – 863Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the dUTPase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000028968.
KOiK01520.
OMAiIACPNAP.
OrthoDBiPOG091H01I6.

Family and domain databases

CDDicd07557. trimeric_dUTPase. 1 hit.
Gene3Di2.70.40.10. 1 hit.
HAMAPiMF_00116. dUTPase_bact. 1 hit.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
IPR033704. dUTPase_trimeric.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR00576. dut. 1 hit.

Sequencei

Sequence statusi: Complete.

B1YVA7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLDLKILDA RMRDYMPAYA TTGSAGLDLR ACLDAPVTLQ PGETTLVPTG 
        60         70         80         90        100
LAIHLADPGY AALILPRSGL GHKHGIVLGN LVGLIDSDYQ GQLMVSTWNR 
       110        120        130        140 
GQTEFVLNPF ERLAQLVIVP VVQAQFNIVD DFAQSERGEG GFGSTGRH
Length:148
Mass (Da):15,930
Last modified:May 20, 2008 - v1
Checksum:iBB1275F53AB1240E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001025 Genomic DNA. Translation: ACB64911.1.
RefSeqiWP_012364518.1. NC_010551.1.

Genome annotation databases

EnsemblBacteriaiACB64911; ACB64911; BamMC406_2433.
KEGGibac:BamMC406_2433.
PATRICi19034405. VBIBurAmb82852_2501.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001025 Genomic DNA. Translation: ACB64911.1.
RefSeqiWP_012364518.1. NC_010551.1.

3D structure databases

ProteinModelPortaliB1YVA7.
SMRiB1YVA7. Positions 2-132.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACB64911; ACB64911; BamMC406_2433.
KEGGibac:BamMC406_2433.
PATRICi19034405. VBIBurAmb82852_2501.

Phylogenomic databases

HOGENOMiHOG000028968.
KOiK01520.
OMAiIACPNAP.
OrthoDBiPOG091H01I6.

Enzyme and pathway databases

UniPathwayiUPA00610; UER00666.
BioCyciBAMB398577:GH38-2503-MONOMER.

Family and domain databases

CDDicd07557. trimeric_dUTPase. 1 hit.
Gene3Di2.70.40.10. 1 hit.
HAMAPiMF_00116. dUTPase_bact. 1 hit.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
IPR033704. dUTPase_trimeric.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR00576. dut. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDUT_BURA4
AccessioniPrimary (citable) accession number: B1YVA7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 20, 2008
Last modified: September 7, 2016
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.