UniProtKB - B1YVA7 (DUT_BURA4)
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Protein
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Gene
dut
Organism
Burkholderia ambifaria (strain MC40-6)
Status
Functioni
This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.UniRule annotation
Catalytic activityi
dUTP + H2O = dUMP + diphosphate.UniRule annotation
Cofactori
Mg2+UniRule annotation
: dUMP biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes dUMP from dCTP (dUTP route).UniRule annotationProteins known to be involved in the 2 steps of the subpathway in this organism are:
- dCTP deaminase (dcd)
- Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dUMP from dCTP (dUTP route), the pathway dUMP biosynthesis and in Pyrimidine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 80 | SubstrateUniRule annotation | 1 | |
Binding sitei | 94 | Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation | 1 |
GO - Molecular functioni
- dUTP diphosphatase activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- dUMP biosynthetic process Source: UniProtKB-UniPathway
- dUTP metabolic process Source: InterPro
Keywordsi
Molecular function | Hydrolase |
Biological process | Nucleotide metabolism |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BioCyci | BAMB398577:G1GBO-2466-MONOMER. |
UniPathwayi | UPA00610; UER00666. |
Names & Taxonomyi
Protein namesi | Recommended name: Deoxyuridine 5'-triphosphate nucleotidohydrolaseUniRule annotation (EC:3.6.1.23UniRule annotation)Short name: dUTPaseUniRule annotation Alternative name(s): dUTP pyrophosphataseUniRule annotation |
Gene namesi | Name:dutUniRule annotation Ordered Locus Names:BamMC406_2433 |
Organismi | Burkholderia ambifaria (strain MC40-6) |
Taxonomic identifieri | 398577 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_1000094944 | 1 – 148 | Deoxyuridine 5'-triphosphate nucleotidohydrolaseAdd BLAST | 148 |
Structurei
3D structure databases
ProteinModelPortali | B1YVA7. |
SMRi | B1YVA7. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 67 – 69 | Substrate bindingUniRule annotation | 3 | |
Regioni | 84 – 86 | Substrate bindingUniRule annotation | 3 |
Sequence similaritiesi
Belongs to the dUTPase family.UniRule annotation
Phylogenomic databases
HOGENOMi | HOG000028968. |
KOi | K01520. |
OMAi | GVILINH. |
OrthoDBi | POG091H01I6. |
Family and domain databases
CDDi | cd07557. trimeric_dUTPase. 1 hit. |
Gene3Di | 2.70.40.10. 1 hit. |
HAMAPi | MF_00116. dUTPase_bact. 1 hit. |
InterProi | View protein in InterPro IPR029054. dUTPase-like. IPR036157. dUTPase-like_sf. IPR008181. dUTPase_1. IPR033704. dUTPase_trimeric. |
Pfami | View protein in Pfam PF00692. dUTPase. 1 hit. |
SUPFAMi | SSF51283. SSF51283. 1 hit. |
TIGRFAMsi | TIGR00576. dut. 1 hit. |
i Sequence
Sequence statusi: Complete.
B1YVA7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKLDLKILDA RMRDYMPAYA TTGSAGLDLR ACLDAPVTLQ PGETTLVPTG
60 70 80 90 100
LAIHLADPGY AALILPRSGL GHKHGIVLGN LVGLIDSDYQ GQLMVSTWNR
110 120 130 140
GQTEFVLNPF ERLAQLVIVP VVQAQFNIVD DFAQSERGEG GFGSTGRH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP001025 Genomic DNA. Translation: ACB64911.1. |
RefSeqi | WP_012364518.1. NC_010551.1. |
Genome annotation databases
EnsemblBacteriai | ACB64911; ACB64911; BamMC406_2433. |
KEGGi | bac:BamMC406_2433. |
Similar proteinsi
Entry informationi
Entry namei | DUT_BURA4 | |
Accessioni | B1YVA7Primary (citable) accession number: B1YVA7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 24, 2009 |
Last sequence update: | May 20, 2008 | |
Last modified: | February 28, 2018 | |
This is version 68 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |