B1YVA5 (LSPA_BURA4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoprotein signal peptidase
EC=3.4.23.36

Alternative name(s):
Prolipoprotein signal peptidase
Signal peptidase II
Short name=SPase II

Gene names

Name:	lspA
Ordered Locus Names:	BamMC406_2431

Organism

Burkholderia ambifaria (strain MC40-6) [Complete proteome] [HAMAP]

Taxonomic identifier

398577 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	166 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein specifically catalyzes the removal of signal peptides from prolipoproteins By similarity. HAMAP MF_00161
Catalytic activity	Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP MF_00161
Pathway	Protein modification; lipoprotein biosynthesis (signal peptide cleavage). HAMAP MF_00161
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_00161.
Sequence similarities	Belongs to the peptidase A8 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 166	166	Lipoprotein signal peptidase HAMAP MF_00161		PRO_1000097235
Regions
	Transmembrane	9 – 29	21	Helical; Potential
	Transmembrane	45 – 65	21	Helical; Potential
	Transmembrane	71 – 91	21	Helical; Potential
	Transmembrane	100 – 120	21	Helical; Potential
	Transmembrane	135 – 155	21	Helical; Potential
Sites
	Active site	117	1	By similarity
	Active site	144	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1YVA5 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: E81D2E510AE80D07	FASTA	166	18,051
	10 20 30 40 50 60 MAKTLSKPAS GALAPWLGIS LIVILFDQLS KIAILKTFAY GAQHALTSFF SLVLVYNRGA 70 80 90 100 110 120 AFGFLSTASG WQRWAFTALG IGATLVICFL LRRHGQQRLF SLSLALILGG ALGNVIDRLV 130 140 150 160 YGHVIDFLDF HVGGWHFPAF NLADSAITVG AVLLVYDELR RVRGSR

References

[1]

"Complete sequence of chromosome 1 of Burkholderia ambifaria MC40-6." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A. , Ramette A., Konstantinidis K., Tiedje J., Richardson P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC40-6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001025 Genomic DNA. Translation: ACB64909.1.
RefSeq	YP_001809125.1. NC_010551.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B1YVA5.
Protein family/group databases
MEROPS	A08.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6177350.
GenomeReviews	Gene locus BamMC406_2431 in contig CP001025_GR.
KEGG	bac:BamMC406_2431.
PATRIC	19034401. VBIBurAmb82852_2499.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG724422.
OMA	SKFLILQ.
ProtClustDB	PRK00376.
Enzyme and pathway databases
BioCyc	BAMB398577:BAMMC406_2431-MONOMER.
Family and domain databases
HAMAP	MF_00161. LspA. [Tree]
InterPro	IPR001872. Peptidase_A8. [Graphical view]
KO	K03101.
Pfam	PF01252. Peptidase_A8. 1 hit. [Graphical view]
PRINTS	PR00781. LIPOSIGPTASE.
TIGRFAMs	TIGR00077. LspA. 1 hit.
PROSITE	PS00855. SPASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSPA_BURA4

Accession

Primary (citable) accession number: B1YVA5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program