B1YV52 (DADA_BURA4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-amino acid dehydrogenase small subunit
EC=1.4.99.1

Gene names

Name:	dadA
Ordered Locus Names:	BamMC406_0944

Organism

Burkholderia ambifaria (strain MC40-6) [Complete proteome] [HAMAP]

Taxonomic identifier

398577 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Oxidative deamination of D-amino acids By similarity. HAMAP MF_01202
Catalytic activity	A D-amino acid + H2O + acceptor = a 2-oxo acid + NH3 + reduced acceptor. HAMAP MF_01202
Cofactor	FAD By similarity. HAMAP MF_01202
Pathway	Amino-acid degradation; D-alanine degradation; NH(3) and pyruvate from D-alanine: step 1/1. HAMAP MF_01202
Subunit structure	Heterodimer of a small and a large subunit By similarity.
Sequence similarities	Belongs to the DadA oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	D-amino-acid dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	D-amino acid dehydrogenase small subunit HAMAP MF_01202		PRO_1000138641
Regions
	Nucleotide binding	3 – 17	15	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	B1YV52 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 1138A11A211DF9DA	FASTA	428	45,970
	10 20 30 40 50 60 MRVVVLGSGV VGVASAYYLA RAGHEVTVID REAGPALETS FANAGQISPG YAAPWAAPGV 70 80 90 100 110 120 PLKAVKWMFE KHAPLAIRLD GTRFQLQWMW QMLRNCTADR YAVNKGRMVR LAEYSRDCLQ 130 140 150 160 170 180 ALRADTGIQY EGRTGGTLQL FRTQQQLDGA AKDIAVLQEA NVPFELLSPA ELKNAEPALA 190 200 210 220 230 240 AVSHKLTGGL RLPGDETGDC QLFTTRLAAL AESLGVKFRY NTPIDALAIA GGRIAGVQCG 250 260 270 280 290 300 SETVRADAYV VALGSYSTSF ISNLMKIPVY PLKGYSITAP IVNDAAAPVS TVLDETYKIA 310 320 330 340 350 360 ITRFDQRIRV GGMAEIVGFD KNLRAARRET LEMCVNDLFP GGGDTSKATF WTGLRPMTPD 370 380 390 400 410 420 GTPIVGRTPV SNLFLNTGHG TLGWTMSCGS GQLLADLISG KKPAIQADDL SVHRYLKDVA GQTRPAYA

References

[1]

"Complete sequence of chromosome 1 of Burkholderia ambifaria MC40-6." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A. , Ramette A., Konstantinidis K., Tiedje J., Richardson P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC40-6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001025 Genomic DNA. Translation: ACB63435.1.
RefSeq	YP_001807651.1. NC_010551.1.
3D structure databases
ProteinModelPortal	B1YV52.
ModBase	Search...
Protein-protein interaction databases
STRING	B1YV52.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6176299.
GenomeReviews	Gene locus BamMC406_0944 in contig CP001025_GR.
KEGG	bac:BamMC406_0944.
PATRIC	19031244. VBIBurAmb82852_0962.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG729204.
OMA	VDRQPAV.
ProtClustDB	PRK00711.
Enzyme and pathway databases
BioCyc	BAMB398577:BAMMC406_0944-MONOMER.
Family and domain databases
HAMAP	MF_01202. DadA. [Tree]
InterPro	IPR023080. D-aa_DH_ssu_DadA. IPR006076. FAD-dep_OxRdtase. [Graphical view]
KO	K00285.
Pfam	PF01266. DAO. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DADA_BURA4

Accession

Primary (citable) accession number: B1YV52

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program