Reviewed, UniProtKB/Swiss-Prot B1YUT2 (SYM_BURA4)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	BamMC406_2350

Organism

Burkholderia ambifaria (strain MC40-6) [Complete proteome] [HAMAP]

Taxonomic identifier

398577 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	720 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_00098
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00098
Subunit structure	Homodimer By similarity. HAMAP MF_00098
Subcellular location	Cytoplasm By similarity HAMAP MF_00098.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 720	720	Methionyl-tRNA synthetase HAMAP MF_00098		PRO_1000093700
Regions
	Domain	614 – 720	107	tRNA-binding
	Motif	27 – 37	11	"HIGH" region HAMAP MF_00098
	Motif	348 – 352	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	158	1	Zinc By similarity
	Metal binding	161	1	Zinc By similarity
	Metal binding	171	1	Zinc By similarity
	Metal binding	174	1	Zinc By similarity
	Binding site	351	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1YUT2-1 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 0FCD1D4FA9B313DF	FASTA	720	79,322
	10 20 30 40 50 60 MSASDLTSVQ ATAPQGRRQI LVTSALPYAN GQIHIGHLVE YIQTDIWVRT LRMHGHEVYY 70 80 90 100 110 120 IGADDTHGTP VMLRAEKEGL TPKQLIDRVW TEHKRDFDSF GVSFDNFYST DSDENRVLSE 130 140 150 160 170 180 SIYLALKENG LIAERAIEQA YDPVKEMFLP DRFIKGECPK CHAKDQYGDN CEVCGSTYLP 190 200 210 220 230 240 TELLNPYSVV SGATPVRKTS THYFFRLSDP RCESFLREWV SGLAQPEATN KMREWLGDAG 250 260 270 280 290 300 EAKLADWDIS RDAPYFGFEI PGAPGKYFYV WLDAPVGYYA SFKNLCDREG IDFDAWIRAG 310 320 330 340 350 360 STAEQYHFIG KDILYFHTLF WPAMLEFSGH RTPTNVFAHG FLTVDGAKMS KSRGTFITAQ 370 380 390 400 410 420 SYIDTGLNPE WLRYYFAAKL NATMEDIDLN LDDFQARVNS DLVGKYVNIA SRAAGFLIKR 430 440 450 460 470 480 FDGRVQDSAM NHPLVAKLRD AIASIAAHYE GREYSRALRQ TMELADEVNA YVDGAKPWEL 490 500 510 520 530 540 AKDPANAVAL HETCSVSLEA FRLLSLALKP VMPRVAEAVE AFFGVAPLAW ADAAKPLSSA 550 560 570 580 590 600 QPIKAYQHLM TRVDPKQIDA LLAANRDSLQ ADAAGAAAAG TTAANAAKDA KSAKANAKAV 610 620 630 640 650 660 AANGADDAPI SIDDFAKVDL RIAKIVACQA VEGSDKLLQL TLDIGEEKTR NVFSGIKSAY 670 680 690 700 710 720 QPEQLVGKLT VMVANLAPRK MKFGLSEGMV LAASAADEKA EPGLYILEPH SGAKPGMRVK

References

[1]

"Complete sequence of chromosome 1 of Burkholderia ambifaria MC40-6." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A. , Ramette A., Konstantinidis K., Tiedje J., Richardson P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001025 Genomic DNA. Translation: ACB64828.1.
RefSeq	YP_001809044.1.
3D structure databases
SMR	B1YUT2. Positions 18-566, 463-708, 601-717.
ModBase	Search...
Genome annotation databases
GeneID	6176707.
GenomeReviews	Gene locus BamMC406_2350 in contig CP001025_GR.
KEGG	bac:BamMC406_2350.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG721667.
OMA	SAYQPEQ.
Family and domain databases
HAMAP	MF_00098. Met_tRNA_synth_type1. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR004495. Met-tRNA-synth_Ia_bsu_C. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR014729. Rossmann-like_a/b/a_fold. IPR002547. tRNA-bd_dom. IPR014758. tRNA-synt_met_N. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit. TIGR00399. metG_C_term. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_BURA4

Accession

Primary (citable) accession number: B1YUT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 20, 2008
Last modified:	February 9, 2010
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)