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B1YUT2 (SYM_BURA4) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 29. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methionine--tRNA ligase
EC=6.1.1.10
Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS
Gene names
Name:metG
Ordered Locus Names:BamMC406_2350
OrganismBurkholderia ambifaria (strain MC40-6) [Complete proteome] [HAMAP]
Taxonomic identifier398577 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length720 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_00098

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00098

Subunit structure

Homodimer By similarity. HAMAP MF_00098

Subcellular location

Cytoplasm By similarity HAMAP MF_00098.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Contains 1 tRNA-binding domain.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
RNA-binding
Zinc
tRNA-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processmethionyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

methionine-tRNA ligase activity

Inferred from electronic annotation. Source: EC

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 720720Methionine--tRNA ligase HAMAP MF_00098
PRO_1000093700

Regions

Domain614 – 720107tRNA-binding
Motif27 – 3711"HIGH" region HAMAP MF_00098
Motif348 – 3525"KMSKS" region HAMAP MF_00098

Sites

Metal binding1581Zinc By similarity
Metal binding1611Zinc By similarity
Metal binding1711Zinc By similarity
Metal binding1741Zinc By similarity
Binding site3511ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B1YUT2 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: 0FCD1D4FA9B313DF

FASTA72079,322
        10         20         30         40         50         60 
MSASDLTSVQ ATAPQGRRQI LVTSALPYAN GQIHIGHLVE YIQTDIWVRT LRMHGHEVYY 

        70         80         90        100        110        120 
IGADDTHGTP VMLRAEKEGL TPKQLIDRVW TEHKRDFDSF GVSFDNFYST DSDENRVLSE 

       130        140        150        160        170        180 
SIYLALKENG LIAERAIEQA YDPVKEMFLP DRFIKGECPK CHAKDQYGDN CEVCGSTYLP 

       190        200        210        220        230        240 
TELLNPYSVV SGATPVRKTS THYFFRLSDP RCESFLREWV SGLAQPEATN KMREWLGDAG 

       250        260        270        280        290        300 
EAKLADWDIS RDAPYFGFEI PGAPGKYFYV WLDAPVGYYA SFKNLCDREG IDFDAWIRAG 

       310        320        330        340        350        360 
STAEQYHFIG KDILYFHTLF WPAMLEFSGH RTPTNVFAHG FLTVDGAKMS KSRGTFITAQ 

       370        380        390        400        410        420 
SYIDTGLNPE WLRYYFAAKL NATMEDIDLN LDDFQARVNS DLVGKYVNIA SRAAGFLIKR 

       430        440        450        460        470        480 
FDGRVQDSAM NHPLVAKLRD AIASIAAHYE GREYSRALRQ TMELADEVNA YVDGAKPWEL 

       490        500        510        520        530        540 
AKDPANAVAL HETCSVSLEA FRLLSLALKP VMPRVAEAVE AFFGVAPLAW ADAAKPLSSA 

       550        560        570        580        590        600 
QPIKAYQHLM TRVDPKQIDA LLAANRDSLQ ADAAGAAAAG TTAANAAKDA KSAKANAKAV 

       610        620        630        640        650        660 
AANGADDAPI SIDDFAKVDL RIAKIVACQA VEGSDKLLQL TLDIGEEKTR NVFSGIKSAY 

       670        680        690        700        710        720 
QPEQLVGKLT VMVANLAPRK MKFGLSEGMV LAASAADEKA EPGLYILEPH SGAKPGMRVK

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References

[1]"Complete sequence of chromosome 1 of Burkholderia ambifaria MC40-6."
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A. expand/collapse author list , Ramette A., Konstantinidis K., Tiedje J., Richardson P.
Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MC40-6.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001025 Genomic DNA. Translation: ACB64828.1.
RefSeqYP_001809044.1. NC_010551.1.

3D structure databases

ProteinModelPortalB1YUT2.
SMRB1YUT2. Positions 18-566.
ModBaseSearch...

Protein-protein interaction databases

STRINGB1YUT2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6176707.
GenomeReviewsGene locus BamMC406_2350 in contig CP001025_GR.
KEGGbac:BamMC406_2350.
PATRIC19034229. VBIBurAmb82852_2414.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG721667.
OMAEEKTRNV.
ProtClustDBPRK00133.

Enzyme and pathway databases

BioCycBAMB398577:BAMMC406_2350-MONOMER.

Family and domain databases

HAMAPMF_00098. Met_tRNA_synth_type1.
[Tree]
InterProIPR015413. aa-tRNA-synt_I.
IPR001412. aa-tRNA-synth_I_CS.
IPR004495. Met-tRNA-synth_Ia_bsu_C.
IPR014758. Met-tRNA_synth.
IPR023458. Met-tRNA_synth_1.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KOK01874.
PfamPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMsTIGR00398. MetG. 1 hit.
TIGR00399. MetG_C_term. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYM_BURA4
AccessionPrimary (citable) accession number: B1YUT2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 20, 2008
Last modified: January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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