Skip Header

Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot B1YU52 (HEM6_BURA4)

Last modified January 19, 2010. Version 15. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Coproporphyrinogen-III oxidase, aerobic
      Short name=Coproporphyrinogenase
      Short name=Coprogen oxidase
    EC=1.3.3.3
Gene names
Name: hemF
Ordered Locus Names: BamMC406_2213
OrganismBurkholderia ambifaria (strain MC40-6) [Complete proteome] [HAMAP]
Taxonomic identifier398577 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Hide | Top

Protein attributes

Sequence length306 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

Hide | Top

General annotation (Comments)

Function

Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III By similarity. HAMAP MF_00333

Catalytic activity

Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O. HAMAP MF_00333

Pathway

Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1. HAMAP MF_00333

Subunit structure

Homodimer By similarity. HAMAP MF_00333

Subcellular location

Cytoplasm By similarity HAMAP MF_00333.

Sequence similarities

Belongs to the aerobic coproporphyrinogen-III oxidase family.

Hide | Top

Ontologies

Keywords
   Biological processPorphyrin biosynthesis
   Cellular componentCytoplasm
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

porphyrin biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncoproporphyrinogen oxidase activity

Inferred from electronic annotation. Source: HAMAP

protein homodimerization activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 306306Coproporphyrinogen-III oxidase, aerobic HAMAP MF_00333
PRO_1000119790

Regions

Region55 – 6410Important for dimerization By similarity
Region115 – 1173Substrate binding By similarity
Region247 – 28236Important for dimerization By similarity
Region265 – 2706Substrate binding By similarity

Sites

Active site1131Proton donor By similarity
Binding site991Substrate By similarity
Site1821Important for dimerization By similarity

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
B1YU52-1 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: 4EE4D08ABFF792A9

FASTA30634,519
        10         20         30         40         50         60 
MTDSTYDVPR VRTYLQGLQT RIADALGALD GTPLATDVWQ RGPEERLRGG GCTRILEGGR 

        70         80         90        100        110        120 
VFERAGIGFS DVAGDALPPS ASAARPQLAG RGFEALGVSL VLHPRNPYCP TVHMNVRMLI 

       130        140        150        160        170        180 
ATKPGEAPIF WFGGGMDLTP VYPFEDDARH FHQVCKDALD PFGAELYPRF KTWCDEYFFL 

       190        200        210        220        230        240 
KHRNETRGIG GIFFDDFSEP GFERSFEMMQ SVGDAFLNAY LPIVERRAAL PYGERERDFQ 

       250        260        270        280        290        300 
AYRRGRYVEF NLVFDRGTLF GLQSGGRTES ILMSMPPVAN WRYNWQPEPG SPEARLSEFL 


VPRDWV

« Hide

Hide | Top

References

[1]"Complete sequence of chromosome 1 of Burkholderia ambifaria MC40-6."
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A. expand/collapse author list , Ramette A., Konstantinidis K., Tiedje J., Richardson P.
Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Hide | Top

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001025 Genomic DNA. Translation: ACB64692.1.
RefSeqYP_001808908.1.

3D structure databases

SMRB1YU52. Positions 5-306.
ModBaseSearch...

Genome annotation databases

GeneID6177910.
GenomeReviewsGene locus BamMC406_2213 in contig CP001025_GR.
KEGGbac:BamMC406_2213.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG631180.
OMAHMNTRFV.

Family and domain databases

HAMAPMF_00333. Coprogen_oxidas.
[Tree]
InterProIPR001260. Coprogen_oxidas.
IPR018375. Coproporphyrinogen-3_ox_CS.
[Graphical view]
Gene3DG3DSA:3.40.1500.10. Coprogen_oxidas. 1 hit.
PANTHERPTHR10755. Coprogen_oxidas. 1 hit.
PfamPF01218. Coprogen_oxidas. 1 hit.
[Graphical view]
PIRSFPIRSF000166. Coproporphyri_ox. 1 hit.
PRINTSPR00073. COPRGNOXDASE.
PROSITEPS01021. COPROGEN_OXIDASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Hide | Top

Entry information

Entry nameHEM6_BURA4
AccessionPrimary (citable) accession number: B1YU52
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: May 20, 2008
Last modified: January 19, 2010
This is version 15 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Hide | Top

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents