B1YTH4 (KATG1_BURA4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase 1
Short name=CP 1
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase 1

Gene names

Name:	katG1
Ordered Locus Names:	BamMC406_0642

Organism

Burkholderia ambifaria (strain MC40-6) [Complete proteome] [HAMAP]

Taxonomic identifier

398577 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	728 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 728	728	Catalase-peroxidase 1 HAMAP MF_01961		PRO_0000354731
Sites
	Active site	92	1	Proton acceptor By similarity
	Metal binding	259	1	Iron (heme axial ligand) By similarity
	Site	88	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	91 ↔ 218		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-244) By similarity
	Cross-link	218 ↔ 244		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-91) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1YTH4 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 6E914122B42278BE	FASTA	728	79,221
	10 20 30 40 50 60 MSTESKCPFN HTAAGGTSNK DWWPNQLNLN ILHRHSALSD PMDKDFDYAQ AFKKLDLAAV 70 80 90 100 110 120 KRDLQALMTT SQDWWPADFG HYGGLFVRMA WHSAGTYRTA DGRGGAGGGQ QRFAPLNSWP 130 140 150 160 170 180 DNANLDKARR LLWPIKQKYG RNISWADLLI LTGNVALESM GFKTFGYAGG RADTWEPDDV 190 200 210 220 230 240 YWGSEKIWLE LSGGPNSRYS GDRELENPLA AVQMGLIYVN PEGPDGNPDP VAAARDIRDT 250 260 270 280 290 300 FARMAMNDEE TVALIAGGHT FGKTHGAGPA TDVGPEPEAA GIEQQGLGWK SAYASGKGHD 310 320 330 340 350 360 AITSGLEVTW TSTPTKWSHD FFKHLFSYEW ELTKSPAGAH QWVAKGADEV IPDAFDASKK 370 380 390 400 410 420 HRPTMLTTDL SLRFDPAYEK ISRRFYENPA EFADAFARAW FKLTHRDMGP RARYLGPEVP 430 440 450 460 470 480 AEELLWQDPI PVVDHPLIDD ADVAALKAKV LASGLSVAQL VSTAWASAST FRGSDKRGGA 490 500 510 520 530 540 NGARIRLAPQ KDWEVNQPAA LAAVLETLEG VRKAFNDAQT GGKKVSLADL IVLAGAAGIE 550 560 570 580 590 600 QAAKNAGVAV TVPFAPGRMD ASQEQTDVDA MAVLEPVADG FRNYLKSAYK TPAEALLVDK 610 620 630 640 650 660 AQLLTLNGPE LTVLVGGLRV LGANVGDAKH GVFTDRPGTL SNDFFVNLLD MGTEWKPVSA 670 680 690 700 710 720 ANDVFEGRDR ATGLVKWTGT RVDLIFGSHS QLRALAEVYG SADANEKFVR DFVAAWDKVM NLDRFDLA

References

[1]

"Complete sequence of chromosome 1 of Burkholderia ambifaria MC40-6." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A. , Ramette A., Konstantinidis K., Tiedje J., Richardson P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC40-6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001025 Genomic DNA. Translation: ACB63139.1.
RefSeq	YP_001807355.1. NC_010551.1.
3D structure databases
ProteinModelPortal	B1YTH4.
SMR	B1YTH4. Positions 16-728.
ModBase	Search...
Protein-protein interaction databases
STRING	B1YTH4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6178921.
GenomeReviews	Gene locus BamMC406_0642 in contig CP001025_GR.
KEGG	bac:BamMC406_0642.
PATRIC	19030598. VBIBurAmb82852_0648.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285610.
OMA	KRHAPSM.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	BAMB398577:BAMMC406_0642-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG1_BURA4

Accession

Primary (citable) accession number: B1YTH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program