Reviewed, UniProtKB/Swiss-Prot B1YSU8 (PROB_BURA4)

Names and origin

Protein names

Recommended name:
    Glutamate 5-kinase
    EC=2.7.2.11
Alternative name(s):
    Gamma-glutamyl kinase
      Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	BamMC406_0512

Organism

Burkholderia ambifaria (strain MC40-6) [Complete proteome] [HAMAP]

Taxonomic identifier

398577 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity HAMAP MF_00456.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Glutamate 5-kinase HAMAP MF_00456		PRO_1000125216
Regions
	Domain	280 – 358	79	PUA

Sequences

	Sequence		Length	Mass (Da)
	B1YSU8-1 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: AC8F9240F37766D3	FASTA	372	39,215
	10 20 30 40 50 60 MRSIIADSKR LVVKVGSSLV TNDGKGLDHA AIGRWAAQIA ALRAQGKEVV LVSSGAIAEG 70 80 90 100 110 120 MQRLGWSKRP REIDELQAAA AVGQMGLAQV YESRFTEHDI RTAQILLTHA DLADRERYLN 130 140 150 160 170 180 ARSTLLTLLR LGVVPIINEN DTVVTDEIKF GDNDTLGALV ANLIEGDALI ILTDQSGLFT 190 200 210 220 230 240 ADPRKDPNAT LVAEASAGAP DLEAMAGGAG SSLGRGGMLT KILAAKRAAH SGANTVIASG 250 260 270 280 290 300 RETDVLVRLA AGEAIGTQLI ARTARMAARK QWMADHLQVR GHVVIDAGAV EKLTAGGKSL 310 320 330 340 350 360 LPIGVIGVQG AFARGEVIAC VGPDGREVAR GLTNYSSAET KLIHRKPSGE IETVLGYMLE 370 PELIHRDNLV LV

References

[1]

"Complete sequence of chromosome 1 of Burkholderia ambifaria MC40-6." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A. , Ramette A., Konstantinidis K., Tiedje J., Richardson P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001025 Genomic DNA. Translation: ACB63009.1.
RefSeq	YP_001807225.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6176526.
GenomeReviews	Gene locus BamMC406_0512 in contig CP001025_GR.
KEGG	bac:BamMC406_0512.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG507643.
OMA	TDVDRLY.
Family and domain databases
HAMAP	MF_00456. ProB. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu_5kinase. IPR011529. Glu_5kinase_bact. IPR019797. Glutamate_5-kinase_CS. IPR005715. ProB. IPR002478. PUA. IPR015947. PUA-like. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_BURA4

Accession

Primary (citable) accession number: B1YSU8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	May 20, 2008
Last modified:	February 9, 2010
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)