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B1YSS2 (MURD_BURA4) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 31. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase

EC=6.3.2.9
Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
Gene names
Name:murD
Ordered Locus Names:BamMC406_0486
OrganismBurkholderia ambifaria (strain MC40-6) [Complete proteome] [HAMAP]
Taxonomic identifier398577 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex

Protein attributes

Sequence length502 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639

Catalytic activity

ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639

Subcellular location

Cytoplasm By similarity HAMAP MF_00639.

Sequence similarities

Belongs to the MurCDEF family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 502502UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639
PRO_1000130832

Regions

Nucleotide binding129 – 1357ATP Potential

Sequences

Sequence LengthMass (Da)Tools
B1YSS2 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: 6B82AB513CE34C3E

FASTA50252,824
        10         20         30         40         50         60 
MFGDRQRPMV LVLGLGESGL AIARWCARHG CRLRIADTRE APPNLAALQA EGIDAEFVGG 

        70         80         90        100        110        120 
PFTPSLLDGG VEIVGLSPGL SPLEPALAAL IAAANERAIA VWGELEFFAQ ALRALGTSGY 

       130        140        150        160        170        180 
QPKVLAITGT NGKTTTTNLT GLLCQRSGKK VAVAGNISPA MLDRLARAID ETALPDVWVL 

       190        200        210        220        230        240 
ELSSFQLETA RTFAPDAAAI LNITQDHLDW HGSFDAYAAA KGRVFGATTT RVLNRDDAAV 

       250        260        270        280        290        300 
MKFAPAAGAA DAARTVTFGL NEPAQQGDYG LSRDNGIAWL VEAVDRDAPD ETTSRRRKRD 

       310        320        330        340        350        360 
GVHTPDIAQK RLMPADALRI RGLHNAANAL AAFALARAID LPAAPLLHAL REYRGEAHRV 

       370        380        390        400        410        420 
EVIATIDDVD YVDDSKGTNV GATVAALDGL AQKIVLIAGG DGKGQDFAPL VAPVARWCRA 

       430        440        450        460        470        480 
VMLIGRDAPA IRDTLAETGV PLADHATLEA AVHAAAELAE PGDAVLLSPA CASLDMFRNY 

       490        500 
AHRADVFRAA VDEIAIDKGA TT 

« Hide

References

[1]"Complete sequence of chromosome 1 of Burkholderia ambifaria MC40-6."
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A. expand/collapse author list , Ramette A., Konstantinidis K., Tiedje J., Richardson P.
Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MC40-6.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001025 Genomic DNA. Translation: ACB62983.1.
RefSeqYP_001807199.1. NC_010551.1.

3D structure databases

ProteinModelPortalB1YSS2.
ModBaseSearch...

Protein-protein interaction databases

STRINGB1YSS2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6178483.
GenomeReviewsGene locus BamMC406_0486 in contig CP001025_GR.
KEGGbac:BamMC406_0486.
PATRIC19030260. VBIBurAmb82852_0481.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG750024.
OMAACASWDM.
ProtClustDBPRK02006.

Enzyme and pathway databases

BioCycBAMB398577:BAMMC406_0486-MONOMER.

Family and domain databases

HAMAPMF_00639. MurD.
[Tree]
InterProIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR016040. NAD(P)-bd_dom.
IPR005762. UDP-N-AcMur-Glu_ligase.
[Graphical view]
Gene3DG3DSA:3.90.190.20. Mur_ligase_C. 1 hit.
G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK01925.
PANTHERPTHR23135:SF2. PTHR23135:SF2. 1 hit.
PfamPF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMSSF53244. Mur_ligase_C. 1 hit.
SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMsTIGR01087. MurD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMURD_BURA4
AccessionPrimary (citable) accession number: B1YSS2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: May 20, 2008
Last modified: January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families