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Reviewed, UniProtKB/Swiss-Prot B1YN51 (ISPE_BURA4)

Last modified February 9, 2010. Version 15. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
      Short name=CMK
    EC=2.7.1.148
Alternative name(s):
    4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase
Gene names
Name: ispE
Ordered Locus Names: BamMC406_2719
OrganismBurkholderia ambifaria (strain MC40-6) [Complete proteome] [HAMAP]
Taxonomic identifier398577 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex

Protein attributes

Sequence length293 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

General annotation (Comments)

Function

Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061

Catalytic activity

ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061

Sequence similarities

Belongs to the GHMP kinase family. IspE subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2932934-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061
PRO_1000092064

Regions

Nucleotide binding99 – 10911ATP Potential

Sites

Active site161 By similarity
Active site1411 By similarity

Sequences

Sequence LengthMass (Da)Tools
B1YN51-1 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: 3ACAB9C42ECEE8E9

FASTA29331,774
        10         20         30         40         50         60 
MTDSTRSLRN CLAPAKLNLF LHITGRRPNG YHDLQSVFQL LNWGDTLHFT LRDDGRVARV 

        70         80         90        100        110        120 
TDVPGVPEES DLVVRAANLL KAHTGTAAGV DIEIDKCLPM GAGLGGGSSD AATTLLALNR 

       130        140        150        160        170        180 
LWQLDLSRTE LQSLAVKLGA DVPFFVFGKN AFAEGIGEEL AEVELPTRWF LVVTPRVHVP 

       190        200        210        220        230        240 
TAEIFSDELL TRNTKPVTIA DFLAQQNSDA GWPDSFGRND MQQVVTSKYA EVAQVVKWLY 

       250        260        270        280        290 
NVTPARMTGS GASVFAAFQS KHEAEAAKAQ LPTGWNGAVA ESLNEHPLFA FAS 

« Hide

References

[1]"Complete sequence of chromosome 1 of Burkholderia ambifaria MC40-6."
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A. expand/collapse author list , Ramette A., Konstantinidis K., Tiedje J., Richardson P.
Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001025 Genomic DNA. Translation: ACB65196.1.
RefSeqYP_001809412.1.

3D structure databases

SMRB1YN51. Positions 11-289.
ModBaseSearch...

Genome annotation databases

GeneID6177179.
GenomeReviewsGene locus BamMC406_2719 in contig CP001025_GR.
KEGGbac:BamMC406_2719.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG734593.
OMALNLFLHV.

Family and domain databases

HAMAPMF_00061. IspE.
[Tree]
InterProIPR006204. GHMP_kinase.
IPR013750. GHMP_kinase_C.
IPR004424. IspE.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
Gene3DG3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
PANTHERPTHR20861:SF2. IspE. 1 hit.
PfamPF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFPIRSF010376. IspE. 1 hit.
TIGRFAMsTIGR00154. ispE. 1 hit.
ProtoNetSearch...

Entry information

Entry nameISPE_BURA4
AccessionPrimary (citable) accession number: B1YN51
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 20, 2008
Last modified: February 9, 2010
This is version 15 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents